Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems
This study evaluated the efficacy of various organic solvents (80% acetone, 80% ethanol, 80% methanol) and distilled water for extracting antioxidant phenolic compounds from turmeric, curry leaf, torch ginger and lemon grass extracts. They were analyzed regarding the total phenol and flavonoid co...
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| Language: | English English English |
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Multidisciplinary Digital Publishing Institute (MDPI)
2018
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| Online Access: | http://irep.iium.edu.my/64187/ http://irep.iium.edu.my/64187/1/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_article.pdf http://irep.iium.edu.my/64187/2/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_scopus.pdf http://irep.iium.edu.my/64187/13/64187_Comparative%20analysis%20of%20chemical%20composition%2C%20antioxidant%20activity%20and%20quantitative%20characterization%20of%20_WoS.pdf |
| _version_ | 1848786163178930176 |
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| author | Sepahpour, Shabnam Selamat, Jinap Abdul Manap, Mohd Yazid Khatib, Alfi Abdull Razis, Ahmad Faizal |
| author_facet | Sepahpour, Shabnam Selamat, Jinap Abdul Manap, Mohd Yazid Khatib, Alfi Abdull Razis, Ahmad Faizal |
| author_sort | Sepahpour, Shabnam |
| building | IIUM Repository |
| collection | Online Access |
| description | This study evaluated the efficacy of various organic solvents (80% acetone, 80% ethanol,
80% methanol) and distilled water for extracting antioxidant phenolic compounds from turmeric,
curry leaf, torch ginger and lemon grass extracts. They were analyzed regarding the total phenol and
flavonoid contents, antioxidant activity and concentration of some phenolic compounds. Antioxidant
activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay
and the ferric reducing antioxidant power (FRAP) assay. Quantification of phenolic compounds
was carried out using high-performance liquid chromatography (HPLC). All the extracts possessed
antioxidant activity, however, the different solvents showed different efficiencies in the extraction
of phenolic compounds. Turmeric showed the highest DPPH values (67.83–13.78%) and FRAP
(84.9–2.3 mg quercetin/g freeze-dried crude extract), followed by curry leaf, torch ginger and lemon
grass. While 80% acetone was shown to be the most efficient solvent for the extraction of total
phenolic compounds from turmeric, torch ginger and lemon grass (221.68, 98.10 and 28.19 mg GA/g
freeze dried crude extract, respectively), for the recovery of phenolic compounds from curry leaf
(92.23 mg GA/g freeze-dried crude extract), 80% ethanol was the most appropriate solvent. Results
of HPLC revealed that the amount of phenolic compounds varied depending on the types of
solvents used. |
| first_indexed | 2025-11-14T17:04:40Z |
| format | Article |
| id | iium-64187 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:04:40Z |
| publishDate | 2018 |
| publisher | Multidisciplinary Digital Publishing Institute (MDPI) |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-641872018-08-01T01:16:51Z http://irep.iium.edu.my/64187/ Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems Sepahpour, Shabnam Selamat, Jinap Abdul Manap, Mohd Yazid Khatib, Alfi Abdull Razis, Ahmad Faizal RS192 Materia Medica-Pharmaceutical Technology TP Chemical technology This study evaluated the efficacy of various organic solvents (80% acetone, 80% ethanol, 80% methanol) and distilled water for extracting antioxidant phenolic compounds from turmeric, curry leaf, torch ginger and lemon grass extracts. They were analyzed regarding the total phenol and flavonoid contents, antioxidant activity and concentration of some phenolic compounds. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and the ferric reducing antioxidant power (FRAP) assay. Quantification of phenolic compounds was carried out using high-performance liquid chromatography (HPLC). All the extracts possessed antioxidant activity, however, the different solvents showed different efficiencies in the extraction of phenolic compounds. Turmeric showed the highest DPPH values (67.83–13.78%) and FRAP (84.9–2.3 mg quercetin/g freeze-dried crude extract), followed by curry leaf, torch ginger and lemon grass. While 80% acetone was shown to be the most efficient solvent for the extraction of total phenolic compounds from turmeric, torch ginger and lemon grass (221.68, 98.10 and 28.19 mg GA/g freeze dried crude extract, respectively), for the recovery of phenolic compounds from curry leaf (92.23 mg GA/g freeze-dried crude extract), 80% ethanol was the most appropriate solvent. Results of HPLC revealed that the amount of phenolic compounds varied depending on the types of solvents used. Multidisciplinary Digital Publishing Institute (MDPI) 2018 Article PeerReviewed application/pdf en http://irep.iium.edu.my/64187/1/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_article.pdf application/pdf en http://irep.iium.edu.my/64187/2/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_scopus.pdf application/pdf en http://irep.iium.edu.my/64187/13/64187_Comparative%20analysis%20of%20chemical%20composition%2C%20antioxidant%20activity%20and%20quantitative%20characterization%20of%20_WoS.pdf Sepahpour, Shabnam and Selamat, Jinap and Abdul Manap, Mohd Yazid and Khatib, Alfi and Abdull Razis, Ahmad Faizal (2018) Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems. Molecules, 23 (2). pp. 1-17. ISSN 1420-3049 http://www.mdpi.com/1420-3049/23/2/402 10.3390/molecules23020402 |
| spellingShingle | RS192 Materia Medica-Pharmaceutical Technology TP Chemical technology Sepahpour, Shabnam Selamat, Jinap Abdul Manap, Mohd Yazid Khatib, Alfi Abdull Razis, Ahmad Faizal Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems |
| title | Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems |
| title_full | Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems |
| title_fullStr | Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems |
| title_full_unstemmed | Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems |
| title_short | Comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems |
| title_sort | comparative analysis of chemical composition, antioxidant activity and quantitative characterization of some phenolic compounds in selected herbs and spices in different solvent extraction systems |
| topic | RS192 Materia Medica-Pharmaceutical Technology TP Chemical technology |
| url | http://irep.iium.edu.my/64187/ http://irep.iium.edu.my/64187/ http://irep.iium.edu.my/64187/ http://irep.iium.edu.my/64187/1/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_article.pdf http://irep.iium.edu.my/64187/2/64187_Comparative%20Analysis%20of%20Chemical%20Composition%2C_scopus.pdf http://irep.iium.edu.my/64187/13/64187_Comparative%20analysis%20of%20chemical%20composition%2C%20antioxidant%20activity%20and%20quantitative%20characterization%20of%20_WoS.pdf |