Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite
A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employ...
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Hindawi
2018
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| Online Access: | http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf |
| _version_ | 1848786094209892352 |
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| author | Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani |
| author_facet | Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani |
| author_sort | Yusan, Sabriye |
| building | IIUM Repository |
| collection | Online Access |
| description | A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high affinity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the effectsofpHvalue,scanrate,mediatoreffectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility. |
| first_indexed | 2025-11-14T17:03:34Z |
| format | Article |
| id | iium-63788 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T17:03:34Z |
| publishDate | 2018 |
| publisher | Hindawi |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-637882018-07-30T02:23:14Z http://irep.iium.edu.my/63788/ Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high affinity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the effectsofpHvalue,scanrate,mediatoreffectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility. Hindawi 2018-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf application/pdf en http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf Yusan, Sabriye and M. Rahman, Mokhlesur and Mohamad, Nasir A.N. and ,, Tengku M. Arrif and Abdul Latif, Ahmad Zubaidi and Md Aris, Mohd Aznan and Wan Nik, Wan Sani (2018) Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite. Journal of Analytical Methods in Chemistry, 2018. pp. 1-8. ISSN 2090-8865 E-ISSN 2090-8873 https://doi.org/10.1155/2018/2687341 10.1155/2018/2687341 |
| spellingShingle | R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine Yusan, Sabriye M. Rahman, Mokhlesur Mohamad, Nasir A.N. ,, Tengku M. Arrif Abdul Latif, Ahmad Zubaidi Md Aris, Mohd Aznan Wan Nik, Wan Sani Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite |
| title | Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41
mesoporous composite |
| title_full | Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41
mesoporous composite |
| title_fullStr | Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41
mesoporous composite |
| title_full_unstemmed | Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41
mesoporous composite |
| title_short | Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41
mesoporous composite |
| title_sort | development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the se-mcm-41
mesoporous composite |
| topic | R Medicine (General) RM Therapeutics. Pharmacology RS Pharmacy and materia medica RZ Other systems of medicine |
| url | http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/ http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf |