Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model
Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal...
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| Format: | Article |
| Language: | English English |
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Wolters Kluwer - Medknow
2018
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| Online Access: | http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/1/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection.pdf http://irep.iium.edu.my/63579/7/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection%20of%20Tocotrienols%20in%20Chronic%20Cerebral%20Hypoperfusion-Induced%20Neurodegeneration%20Rat%20Animal%20Model_SCOPUS.pdf |
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| author | Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman |
| author_facet | Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman |
| author_sort | Mohamed, Wael Mohamed Yousef |
| building | IIUM Repository |
| collection | Online Access |
| description | Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal model. Materials and Methods: Twenty-four Sprague Dawley rats weighing 200–250 g were equally divided into the following three groups: SHAM control, 2VO, and 2VO+E (treated daily with vitamin E tocotrienol, 100 mg/kg, orally following 2VO). On the 8th week after 2VO surgery, rats were euthanized and the hippocampi were isolated with the estimation of viable neuronal cell count in the hippocampal CA-1 region. The isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels. Results: There was significantly higher neuronal cell death in the hippocampal CA-1 region and increased Iso-F2 levels in the 2VO group compared to the SHAM control group (P < 0.05). Conversely, no significant difference was observed with regard to the neuronal cell death and Iso-F2 levels in the 2VO+E group and the SHAM control group (P > 0.05). Conclusion: This study demonstrates the effectiveness of vitamin E tocotrienol as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. |
| first_indexed | 2025-11-14T17:02:58Z |
| format | Article |
| id | iium-63579 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:02:58Z |
| publishDate | 2018 |
| publisher | Wolters Kluwer - Medknow |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-635792018-07-24T01:23:36Z http://irep.iium.edu.my/63579/ Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman R Medicine (General) Background: Reduced cerebral blood flow is associated with aging, neurodegenerative disorders, and an increased formation of reactive oxygen species. This study was designed to assess the potential use of vitamin E as an antioxidant and neuroprotective agent using 2-vessel occlusion (2VO) rat animal model. Materials and Methods: Twenty-four Sprague Dawley rats weighing 200–250 g were equally divided into the following three groups: SHAM control, 2VO, and 2VO+E (treated daily with vitamin E tocotrienol, 100 mg/kg, orally following 2VO). On the 8th week after 2VO surgery, rats were euthanized and the hippocampi were isolated with the estimation of viable neuronal cell count in the hippocampal CA-1 region. The isoprostane F2 (Iso-F2) levels were also measured in the brain homogenates to quantify the oxidative stress levels. Results: There was significantly higher neuronal cell death in the hippocampal CA-1 region and increased Iso-F2 levels in the 2VO group compared to the SHAM control group (P < 0.05). Conversely, no significant difference was observed with regard to the neuronal cell death and Iso-F2 levels in the 2VO+E group and the SHAM control group (P > 0.05). Conclusion: This study demonstrates the effectiveness of vitamin E tocotrienol as a neuroprotective and antioxidant agent in chronic cerebral hypoperfusion-induced neurodegeneration in rats. Wolters Kluwer - Medknow 2018-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63579/1/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection.pdf application/pdf en http://irep.iium.edu.my/63579/7/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection%20of%20Tocotrienols%20in%20Chronic%20Cerebral%20Hypoperfusion-Induced%20Neurodegeneration%20Rat%20Animal%20Model_SCOPUS.pdf Mohamed, Wael Mohamed Yousef and Sayeed, Sayyada and Saxena, Anil Kumar and Syed Ahmad, Pakeer Oothuman (2018) Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model. International Journal of Nutrition, Pharmacology, Neurological Diseases, 8 (2). pp. 47-52. ISSN 2231-0738 E-ISSN 2231-2722 http://www.ijnpnd.com/article.asp?issn=2231-0738;year=2018;volume=8;issue=2;spage=47;epage=52;aulast=Mohamed;type=0 10.4103/ijnpnd.ijnpnd_17_18 |
| spellingShingle | R Medicine (General) Mohamed, Wael Mohamed Yousef Sayeed, Sayyada Saxena, Anil Kumar Syed Ahmad, Pakeer Oothuman Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model |
| title | Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model |
| title_full | Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model |
| title_fullStr | Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model |
| title_full_unstemmed | Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model |
| title_short | Oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model |
| title_sort | oxidative stress status and neuroprotection of tocotrienols in chronic cerebral hypoperfusion-induced neurodegeneration rat animal model |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/ http://irep.iium.edu.my/63579/1/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection.pdf http://irep.iium.edu.my/63579/7/63579_Oxidative%20Stress%20Status%20and%20Neuroprotection%20of%20Tocotrienols%20in%20Chronic%20Cerebral%20Hypoperfusion-Induced%20Neurodegeneration%20Rat%20Animal%20Model_SCOPUS.pdf |