The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan
Objective: The present study is designed to investigate the occurrence of Acanthamoeba in air ventilation and air-conditioning systems in selected buildings in Kuantan. Methods: Acanthamoeba was isolated from dust samples taken from filters of air ventilation systems in selected buildings in Kuantan...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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IIUM Press
2018
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| Online Access: | http://irep.iium.edu.my/63303/ http://irep.iium.edu.my/63303/7/63303%20The%20Determination%20of%20Acanthamoeba.pdf |
| _version_ | 1848786009525846016 |
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| author | Md Isa, Muhammad Lokman Aznan, Anis Amira Mat Amin, Nakisah Mat Yusof, Afzan |
| author_facet | Md Isa, Muhammad Lokman Aznan, Anis Amira Mat Amin, Nakisah Mat Yusof, Afzan |
| author_sort | Md Isa, Muhammad Lokman |
| building | IIUM Repository |
| collection | Online Access |
| description | Objective: The present study is designed to investigate the occurrence of Acanthamoeba in air ventilation and air-conditioning systems in selected buildings in Kuantan. Methods: Acanthamoeba was isolated from dust samples taken from filters of air ventilation systems in selected buildings in Kuantan. The dusts were collected by using sterile cotton swabs, cultured in a xenic culture medium on non-nutrient agar (NNA) plates and incubated at 37º C. The plates were examined daily for any presence of Acanthamoeba cysts up to 7 days of incubation. Results: Based on the results obtained, there were no cysts or trophozoites of Acanthamoeba successfully isolated from all 75 dust samples. After three days of incubation, all culture plates examined show negative findings. Conclusions: The negative findings of this study were probably due to the limitations of the sampling method. It is recommended that future studies use the method proposed by National Institute of Occupational Safety and Health (NIOSH) for indoor air quality monitoring. |
| first_indexed | 2025-11-14T17:02:13Z |
| format | Article |
| id | iium-63303 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T17:02:13Z |
| publishDate | 2018 |
| publisher | IIUM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-633032025-03-19T07:02:13Z http://irep.iium.edu.my/63303/ The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan Md Isa, Muhammad Lokman Aznan, Anis Amira Mat Amin, Nakisah Mat Yusof, Afzan Q Science (General) Objective: The present study is designed to investigate the occurrence of Acanthamoeba in air ventilation and air-conditioning systems in selected buildings in Kuantan. Methods: Acanthamoeba was isolated from dust samples taken from filters of air ventilation systems in selected buildings in Kuantan. The dusts were collected by using sterile cotton swabs, cultured in a xenic culture medium on non-nutrient agar (NNA) plates and incubated at 37º C. The plates were examined daily for any presence of Acanthamoeba cysts up to 7 days of incubation. Results: Based on the results obtained, there were no cysts or trophozoites of Acanthamoeba successfully isolated from all 75 dust samples. After three days of incubation, all culture plates examined show negative findings. Conclusions: The negative findings of this study were probably due to the limitations of the sampling method. It is recommended that future studies use the method proposed by National Institute of Occupational Safety and Health (NIOSH) for indoor air quality monitoring. IIUM Press 2018-01-31 Article PeerReviewed application/pdf en http://irep.iium.edu.my/63303/7/63303%20The%20Determination%20of%20Acanthamoeba.pdf Md Isa, Muhammad Lokman and Aznan, Anis Amira and Mat Amin, Nakisah and Mat Yusof, Afzan (2018) The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan. International Journal of Care Scholars, 1 (1). pp. 19-21. E-ISSN 2600-898X http://journals.iium.edu.my/ijcs/index.php/ijcs/article/view/40/5 10.31436/ijcs.v1i1.40 |
| spellingShingle | Q Science (General) Md Isa, Muhammad Lokman Aznan, Anis Amira Mat Amin, Nakisah Mat Yusof, Afzan The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan |
| title | The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan |
| title_full | The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan |
| title_fullStr | The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan |
| title_full_unstemmed | The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan |
| title_short | The determination of Acanthamoeba spp. from air ventilation system samples from selected buildings in Kuantan |
| title_sort | determination of acanthamoeba spp. from air ventilation system samples from selected buildings in kuantan |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/63303/ http://irep.iium.edu.my/63303/ http://irep.iium.edu.my/63303/ http://irep.iium.edu.my/63303/7/63303%20The%20Determination%20of%20Acanthamoeba.pdf |