Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres
Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycol...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English English |
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MDPI AG
2017
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| Online Access: | http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/1/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_article.pdf http://irep.iium.edu.my/62789/2/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_scopus.pdf |
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| author | Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed |
| author_facet | Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed |
| author_sort | Ansary, Rezaul H. |
| building | IIUM Repository |
| collection | Online Access |
| description | Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential
delivery systems for reducing a very high initial burst release of encapsulated protein and peptide
drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated
poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using
a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for
the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation
efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated.
Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising
of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces.
A statistically significant increase in the encapsulation efficiency (82.52 ± 3.28%) was achieved when
1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and
external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared
together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released
lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres
fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA
and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release
for lysozyme. |
| first_indexed | 2025-11-14T17:00:36Z |
| format | Article |
| id | iium-62789 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T17:00:36Z |
| publishDate | 2017 |
| publisher | MDPI AG |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-627892018-06-21T02:34:22Z http://irep.iium.edu.my/62789/ Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed QD Chemistry QR Microbiology RE Ophthalmology RS Pharmacy and materia medica Double-walled microspheres based on poly(lactide-co-glycolide) (PLGA) are potential delivery systems for reducing a very high initial burst release of encapsulated protein and peptide drugs. In this study, double-walled microspheres made of glucose core, hydroxyl-terminated poly(lactide-co-glycolide) (Glu-PLGA), and carboxyl-terminated PLGA were fabricated using a modified water-in-oil-in-oil-in-water (w1/o/o/w2) emulsion solvent evaporation technique for the controlled release of a model protein, lysozyme. Microspheres size, morphology, encapsulation efficiency, lysozyme in vitro release profiles, bioactivity, and structural integrity, were evaluated. Scanning electron microscopy (SEM) images revealed that double-walled microspheres comprising of Glu-PLGA and PLGA with a mass ratio of 1:1 have a spherical shape and smooth surfaces. A statistically significant increase in the encapsulation efficiency (82.52 ± 3.28%) was achieved when 1% (w/v) polyvinyl alcohol (PVA) and 2.5% (w/v) trehalose were incorporated in the internal and external aqueous phase, respectively, during emulsification. Double-walled microspheres prepared together with excipients (PVA and trehalose) showed a better control release of lysozyme. The released lysozyme was fully bioactive, and its structural integrity was slightly affected during microspheres fabrication and in vitro release studies. Therefore, double-walled microspheres made of Glu-PLGA and PLGA together with excipients (PVA and trehalose) provide a controlled and sustained release for lysozyme. MDPI AG 2017-10-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/62789/1/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_article.pdf application/pdf en http://irep.iium.edu.my/62789/2/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_scopus.pdf Ansary, Rezaul H. and Rahman, Mohd. Mokhlesur and Mohamad, Nasir A.N. and Arrif, Tengku M and Abdul Latif, Ahmad Zubaidi and Katas, Haliza and Wan Nik, Wan Sani and Awang, Mohamed (2017) Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres. Polymers, 9 (10). pp. 1-17. ISSN 2073-4360 http://www.mdpi.com/2073-4360/9/10/485/htm 10.3390/polym9100485 |
| spellingShingle | QD Chemistry QR Microbiology RE Ophthalmology RS Pharmacy and materia medica Ansary, Rezaul H. Rahman, Mohd. Mokhlesur Mohamad, Nasir A.N. Arrif, Tengku M Abdul Latif, Ahmad Zubaidi Katas, Haliza Wan Nik, Wan Sani Awang, Mohamed Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres |
| title | Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres |
| title_full | Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres |
| title_fullStr | Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres |
| title_full_unstemmed | Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres |
| title_short | Controlled release of lysozyme from double-walled poly(Lactide-Co-Glycolide) (PLGA) microspheres |
| title_sort | controlled release of lysozyme from double-walled poly(lactide-co-glycolide) (plga) microspheres |
| topic | QD Chemistry QR Microbiology RE Ophthalmology RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/ http://irep.iium.edu.my/62789/1/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_article.pdf http://irep.iium.edu.my/62789/2/62789_Controlled%20Release%20of%20Lysozyme%20from%20Double-Walled_scopus.pdf |