Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment
Aortic scaffolds prepared using sonication decellularization treatment has provided a successful medium for repopulation with vascular smooth muscle cells (VSMCs). The objective of this study is to explore the potential of tissue decellularization using ultrasonication treatment and its recellulariz...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
Institute of Electrical and Electronics Engineers Inc.
2017
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| Online Access: | http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/1/61883_Evaluation%20of%20Recellularization%20on%20Decellularized.pdf http://irep.iium.edu.my/61883/2/61883_Evaluation%20of%20Recellularization%20on%20Decellularized_SCOPUS.pdf |
| _version_ | 1848785755578564608 |
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| author | Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim |
| author_facet | Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim |
| author_sort | Ismail, Nurul Fitriatul Akmal |
| building | IIUM Repository |
| collection | Online Access |
| description | Aortic scaffolds prepared using sonication decellularization treatment has provided a successful medium for repopulation with vascular smooth muscle cells (VSMCs). The objective of this study is to explore the potential of tissue decellularization using ultrasonication treatment and its recellularization before implantation of the cell-seeded scaffolds into host. Aorta tissue samples are decellularized in 2% SDS with sonication for 10 hours and compared with the
native tissues. The 4',6-diamidino-2-phenylindole (DAPI)
staining was used to evaluate the decellularization and
Hematoxylin-Eosin (H-E) staining was used to compare the
VSMCs infiltrations onto the decellularized tissues at day-0 and day-6 after cell-seeding. The results histologically showed complete DNA removal from scaffolds after decellularization and subsequent recellularization resulted in successful VSMCs infiltration. Accordingly, the decellularized tissues treated with 2% SDS in sonication demonstrated successful VSMCs repopulation afterward and is speculated to have less toxicity
and able to be effectively implanted into host. |
| first_indexed | 2025-11-14T16:58:11Z |
| format | Proceeding Paper |
| id | iium-61883 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:58:11Z |
| publishDate | 2017 |
| publisher | Institute of Electrical and Electronics Engineers Inc. |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-618832018-06-26T04:24:51Z http://irep.iium.edu.my/61883/ Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim TP248.13 Biotechnology Aortic scaffolds prepared using sonication decellularization treatment has provided a successful medium for repopulation with vascular smooth muscle cells (VSMCs). The objective of this study is to explore the potential of tissue decellularization using ultrasonication treatment and its recellularization before implantation of the cell-seeded scaffolds into host. Aorta tissue samples are decellularized in 2% SDS with sonication for 10 hours and compared with the native tissues. The 4',6-diamidino-2-phenylindole (DAPI) staining was used to evaluate the decellularization and Hematoxylin-Eosin (H-E) staining was used to compare the VSMCs infiltrations onto the decellularized tissues at day-0 and day-6 after cell-seeding. The results histologically showed complete DNA removal from scaffolds after decellularization and subsequent recellularization resulted in successful VSMCs infiltration. Accordingly, the decellularized tissues treated with 2% SDS in sonication demonstrated successful VSMCs repopulation afterward and is speculated to have less toxicity and able to be effectively implanted into host. Institute of Electrical and Electronics Engineers Inc. 2017-07 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/61883/1/61883_Evaluation%20of%20Recellularization%20on%20Decellularized.pdf application/pdf en http://irep.iium.edu.my/61883/2/61883_Evaluation%20of%20Recellularization%20on%20Decellularized_SCOPUS.pdf Ismail, Nurul Fitriatul Akmal and Sha'ban, Munirah and Noor Azmi, Azran Azhim (2017) Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment. In: 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2017), 11th-15th July 2017, Jeju Island, South Korea. http://ieeexplore.ieee.org/document/8037261/ 10.1109/EMBC.2017.8037261 |
| spellingShingle | TP248.13 Biotechnology Ismail, Nurul Fitriatul Akmal Sha'ban, Munirah Noor Azmi, Azran Azhim Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment |
| title | Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment |
| title_full | Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment |
| title_fullStr | Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment |
| title_full_unstemmed | Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment |
| title_short | Evaluation of recellularization on decellularized Aorta Scaffolds engineered by ultrasonication treatment |
| title_sort | evaluation of recellularization on decellularized aorta scaffolds engineered by ultrasonication treatment |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/ http://irep.iium.edu.my/61883/1/61883_Evaluation%20of%20Recellularization%20on%20Decellularized.pdf http://irep.iium.edu.my/61883/2/61883_Evaluation%20of%20Recellularization%20on%20Decellularized_SCOPUS.pdf |