Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes
The aim of this study was to investigate the effect of bottle gourd which belong to family of cucurbitaceae (Lagenaria Siceraria (LS) on hyperglycemia by assessing the cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin using 3T3-L1 adipocytes as a model. Fresh bottle g...
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| Language: | English English |
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MATTIOLI 1885
2019
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| Online Access: | http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/1/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_article.pdf http://irep.iium.edu.my/61714/2/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_wos.pdf |
| _version_ | 1848785719765499904 |
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| author | Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher |
| author_facet | Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher |
| author_sort | Sassi, Assia |
| building | IIUM Repository |
| collection | Online Access |
| description | The aim of this study was to investigate the effect of bottle gourd which belong to family of cucurbitaceae (Lagenaria Siceraria (LS) on hyperglycemia by assessing the cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin using 3T3-L1 adipocytes as a model. Fresh bottle gourd were washed thoroughly with distilled water and segregated into three parts i.e. whole vegetable (LSW), peels (LSP) and seeds (LSS) and were separately blended with water and ethanol. Thus obtained six types of extracts i.e. three for water whole vegetable (LSWw), peels (LSPw), seeds (LSSw) and three for ethanol whole vegetable (LSWe), peels (LSPe) and seeds (LSSe). The 3T3-L1 adipocytes were treated with these extracts for cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin. The collected data was compiled and statistical analysis were performed. Mainly, one-way analysis of variance (ANOVA) were performed followed by Tukey’s post-hoc test to ascertain differences in the means. The lipid droplet formation was significantly (P<0.001) higher in the adipocytes treated with the extracts of LSWw, LSSw, LSWe, LSWe, LSSe and lower in the extracts of LS peels (LSPw, LSPe) compared to induction medium (MDI)/insulin (control). The extracts also significantly (P<0.001) increased glycerol release during adipolysis compared to the control. It caused a significant (P<0.001) increase in adiponectin concentrations for LSPw, LSWw and a decrease in leptin concentrations for the water extracts of the LSPw. The present study showed that there was hypoglycaemic effect of LS extract by improving the regulation of adipogenesis, adipolysis, glucose uptake, adiponectin and leptin in the adipocytes mainly due to the seeds. |
| first_indexed | 2025-11-14T16:57:37Z |
| format | Article |
| id | iium-61714 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:57:37Z |
| publishDate | 2019 |
| publisher | MATTIOLI 1885 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-617142020-09-10T04:39:50Z http://irep.iium.edu.my/61714/ Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher TX341 Nutrition. Foods and food supply The aim of this study was to investigate the effect of bottle gourd which belong to family of cucurbitaceae (Lagenaria Siceraria (LS) on hyperglycemia by assessing the cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin using 3T3-L1 adipocytes as a model. Fresh bottle gourd were washed thoroughly with distilled water and segregated into three parts i.e. whole vegetable (LSW), peels (LSP) and seeds (LSS) and were separately blended with water and ethanol. Thus obtained six types of extracts i.e. three for water whole vegetable (LSWw), peels (LSPw), seeds (LSSw) and three for ethanol whole vegetable (LSWe), peels (LSPe) and seeds (LSSe). The 3T3-L1 adipocytes were treated with these extracts for cells viability, adipogenesis, adipolysis, glucose uptake, adiponectin and leptin. The collected data was compiled and statistical analysis were performed. Mainly, one-way analysis of variance (ANOVA) were performed followed by Tukey’s post-hoc test to ascertain differences in the means. The lipid droplet formation was significantly (P<0.001) higher in the adipocytes treated with the extracts of LSWw, LSSw, LSWe, LSWe, LSSe and lower in the extracts of LS peels (LSPw, LSPe) compared to induction medium (MDI)/insulin (control). The extracts also significantly (P<0.001) increased glycerol release during adipolysis compared to the control. It caused a significant (P<0.001) increase in adiponectin concentrations for LSPw, LSWw and a decrease in leptin concentrations for the water extracts of the LSPw. The present study showed that there was hypoglycaemic effect of LS extract by improving the regulation of adipogenesis, adipolysis, glucose uptake, adiponectin and leptin in the adipocytes mainly due to the seeds. MATTIOLI 1885 2019-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/61714/1/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_article.pdf application/pdf en http://irep.iium.edu.my/61714/2/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_wos.pdf Sassi, Assia and Khattak, Muhammad Muzaffar Ali Khan and Bakhtiar, M. Taher (2019) Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes. Progress in Nutrition, 21 (Supplement 2 (2019)). pp. 238-245. E-ISSN 1129-8723 https://mattioli1885journals.com/index.php/progressinnutrition/article/view/6367/8498 10.23751/pn.v21i2-S.6367 |
| spellingShingle | TX341 Nutrition. Foods and food supply Sassi, Assia Khattak, Muhammad Muzaffar Ali Khan Bakhtiar, M. Taher Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes |
| title | Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes |
| title_full | Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes |
| title_fullStr | Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes |
| title_full_unstemmed | Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes |
| title_short | Bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3T3-L1 adipocytes |
| title_sort | bottle gourd (lagenaria siceraria) extracts improve glucose and lipid metabolism in 3t3-l1 adipocytes |
| topic | TX341 Nutrition. Foods and food supply |
| url | http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/ http://irep.iium.edu.my/61714/1/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_article.pdf http://irep.iium.edu.my/61714/2/61714_Bottle%20gourd%20%28lagenaria%20siceraria%29_wos.pdf |