In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf
Tetracera macrophylla vall. (Dilleniaceae), has been a neglected species from Tetracera genus despite its usefulness in the traditional herbal medicines. T. macrophylla leaves crude ethanol (95%) extract (ETM) and its different fractions obtained through liquid-liquid partitioning strategy were scre...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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2017
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| Online Access: | http://irep.iium.edu.my/61450/ http://irep.iium.edu.my/61450/14/61450-IN%20VITRO%20%CE%91-%20GLUCOSIDASE.pdf http://irep.iium.edu.my/61450/2/fatimah%20presentation_UKM%20Conference%2022%20Nov%202017.pdf |
| _version_ | 1848785674835066880 |
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| author | Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Latip, Jalifah Alhassan, Muhammad |
| author_facet | Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Latip, Jalifah Alhassan, Muhammad |
| author_sort | Opeyemi Roheem, Fatimah |
| building | IIUM Repository |
| collection | Online Access |
| description | Tetracera macrophylla vall. (Dilleniaceae), has been a neglected species from Tetracera genus despite its usefulness in the traditional herbal medicines. T. macrophylla leaves crude ethanol (95%) extract (ETM) and its different fractions obtained through liquid-liquid partitioning strategy were screened for their total phenolics and flavonoids contents and then investigated using in vitro models for their inhibitory activity on α-glucosidase. Free radical scavenging techniques including 2, 2diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were used to examine the antioxidant capacity. ETM was found to be rich in both phenolic and flavonoid contents (417.90 ± 20.16, 5.86 ± 0.51 µg gallic acid (GA) and quercetin (QU) equivalent/mg dry weight) and exhibited good radical scavenging activity on DPPH and ABTS radicals ( 78.00% and 79.0%, respectively). Ethyl acetate fraction exhibited highest phenolic and flavonoid contents (206.34 ± 4.86, 14.01± 1.20 µg GA and QU equivalent/mg dry weight, respectively), and it was also observed to exhibit highest scavenging capacity on DPPH and ABTS radicals viz., 96.73%, 76.51% respectively. Both ETM and ethyl acetate fraction showed a promising and concentration-dependent α-glucosidase inhibitory activity. Bioassay guided silica gel column chromatography purification of ethyl-acetate fraction afforded a triterpenoid (12 mg), and a flavonoid (10 mg) characterized as betullinic acid and 5,7-dihydroxy-8-methoxy flavone based on spectroscopic analysis (IR, NMR). These compounds displayed promising radical scavenging activity against DPPH and ABTS radicals with moderate inhibitory activity on α-glucosidase enzymes. |
| first_indexed | 2025-11-14T16:56:54Z |
| format | Proceeding Paper |
| id | iium-61450 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:56:54Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-614502018-01-18T01:17:16Z http://irep.iium.edu.my/61450/ In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Latip, Jalifah Alhassan, Muhammad Q Science (General) QD Chemistry Tetracera macrophylla vall. (Dilleniaceae), has been a neglected species from Tetracera genus despite its usefulness in the traditional herbal medicines. T. macrophylla leaves crude ethanol (95%) extract (ETM) and its different fractions obtained through liquid-liquid partitioning strategy were screened for their total phenolics and flavonoids contents and then investigated using in vitro models for their inhibitory activity on α-glucosidase. Free radical scavenging techniques including 2, 2diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azinobis-3-ethylbenzothiazine-6-sulfonic acid (ABTS) were used to examine the antioxidant capacity. ETM was found to be rich in both phenolic and flavonoid contents (417.90 ± 20.16, 5.86 ± 0.51 µg gallic acid (GA) and quercetin (QU) equivalent/mg dry weight) and exhibited good radical scavenging activity on DPPH and ABTS radicals ( 78.00% and 79.0%, respectively). Ethyl acetate fraction exhibited highest phenolic and flavonoid contents (206.34 ± 4.86, 14.01± 1.20 µg GA and QU equivalent/mg dry weight, respectively), and it was also observed to exhibit highest scavenging capacity on DPPH and ABTS radicals viz., 96.73%, 76.51% respectively. Both ETM and ethyl acetate fraction showed a promising and concentration-dependent α-glucosidase inhibitory activity. Bioassay guided silica gel column chromatography purification of ethyl-acetate fraction afforded a triterpenoid (12 mg), and a flavonoid (10 mg) characterized as betullinic acid and 5,7-dihydroxy-8-methoxy flavone based on spectroscopic analysis (IR, NMR). These compounds displayed promising radical scavenging activity against DPPH and ABTS radicals with moderate inhibitory activity on α-glucosidase enzymes. 2017 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/61450/14/61450-IN%20VITRO%20%CE%91-%20GLUCOSIDASE.pdf application/pdf en http://irep.iium.edu.my/61450/2/fatimah%20presentation_UKM%20Conference%2022%20Nov%202017.pdf Opeyemi Roheem, Fatimah and Mat So'ad, Siti Zaiton and Ahmed, Qamar Uddin and Latip, Jalifah and Alhassan, Muhammad (2017) In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf. In: University Kebangsaan Malaysia-Universiti Riau, Indonesia-Universitas Islam Indonesia-Prince of Songkla University, Thailand Joint Seminar 2017 (UKM-UR-UII-PSU Joint Seminar 2017), 20th-22nd November 2017, Bangi, Selangor. (Unpublished) |
| spellingShingle | Q Science (General) QD Chemistry Opeyemi Roheem, Fatimah Mat So'ad, Siti Zaiton Ahmed, Qamar Uddin Latip, Jalifah Alhassan, Muhammad In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf |
| title | In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf |
| title_full | In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf |
| title_fullStr | In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf |
| title_full_unstemmed | In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf |
| title_short | In vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from Tetracera macrophylla vall leaf |
| title_sort | in vitro α- glucosidase inhibitory and antioxidant activities of extract, fractions and isolated compounds from tetracera macrophylla vall leaf |
| topic | Q Science (General) QD Chemistry |
| url | http://irep.iium.edu.my/61450/ http://irep.iium.edu.my/61450/14/61450-IN%20VITRO%20%CE%91-%20GLUCOSIDASE.pdf http://irep.iium.edu.my/61450/2/fatimah%20presentation_UKM%20Conference%2022%20Nov%202017.pdf |