Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7)
Natural products from plant have been used for centuries to prevent various diseases including cancer. Many chemopreventive agents are believed to inhibit or delay the progression of cancer cells through suppression of cell proliferation, induction of apoptosis and cell cycle arrest. In Malaysia, b...
| Main Authors: | , , , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/61123/ http://irep.iium.edu.my/61123/6/61123_Cancer%20Chemoprevention%20Study%20of%20Luffa%20Aegyptia_complete.pdf |
| _version_ | 1848785615523414016 |
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| author | Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan |
| author_facet | Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan |
| author_sort | Hassan, Habibah |
| building | IIUM Repository |
| collection | Online Access |
| description | Natural products from plant have been used for centuries to prevent various diseases including cancer. Many chemopreventive agents are believed to inhibit or delay the progression of cancer cells through suppression of cell proliferation, induction of apoptosis and cell cycle arrest. In Malaysia, breast cancer is the most common form of cancer affecting women. The prognosis for breast cancer is still poor and conventional treatment is not always effective. Therefore, this study aims to determine the chemopreventive effect of Luffa aegyptiaca seed extract (LSE) on breast cancer through anti-proliferative effect and its mechanisms on breast cancer cell (MCF-7) as evidenced by the result of IC50 determination, cell proliferation assay and flow cytometer analysis on apoptosis induction and cell cycle arrest. MCF-7 cells were plated at a density of 4.7 x 104 cells/ml in 6–well plate. Trypan blue exclusion method was used to determine cell viability. Untreated and LSE-treated MCF-7 cells were observed for morphological changes with an inverted-phase contrast microscope. The induction of apoptosis and cell cycle arrest was analysed using flow cytometry. LSE showed growth inhibitory effect with IC50 value at 0.0625 mg/ml. Moreover, LSE, show no toxic effect on normal cells possibly showing that it is a potential chemopreventive agent on MCF-7 via the induction of apoptosis and cell cycle arrest. Overall, the data collected provides new insight for the use of Luffa aegyptiaca seed as a chmopreventive agent on MCF-7.
Keywords: Luffa aegyptiaca; anti-proliferative effect; apoptosis; cell cycle; MCF-7 |
| first_indexed | 2025-11-14T16:55:58Z |
| format | Proceeding Paper |
| id | iium-61123 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:55:58Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-611232018-01-23T06:56:21Z http://irep.iium.edu.my/61123/ Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan SF Animal culture Natural products from plant have been used for centuries to prevent various diseases including cancer. Many chemopreventive agents are believed to inhibit or delay the progression of cancer cells through suppression of cell proliferation, induction of apoptosis and cell cycle arrest. In Malaysia, breast cancer is the most common form of cancer affecting women. The prognosis for breast cancer is still poor and conventional treatment is not always effective. Therefore, this study aims to determine the chemopreventive effect of Luffa aegyptiaca seed extract (LSE) on breast cancer through anti-proliferative effect and its mechanisms on breast cancer cell (MCF-7) as evidenced by the result of IC50 determination, cell proliferation assay and flow cytometer analysis on apoptosis induction and cell cycle arrest. MCF-7 cells were plated at a density of 4.7 x 104 cells/ml in 6–well plate. Trypan blue exclusion method was used to determine cell viability. Untreated and LSE-treated MCF-7 cells were observed for morphological changes with an inverted-phase contrast microscope. The induction of apoptosis and cell cycle arrest was analysed using flow cytometry. LSE showed growth inhibitory effect with IC50 value at 0.0625 mg/ml. Moreover, LSE, show no toxic effect on normal cells possibly showing that it is a potential chemopreventive agent on MCF-7 via the induction of apoptosis and cell cycle arrest. Overall, the data collected provides new insight for the use of Luffa aegyptiaca seed as a chmopreventive agent on MCF-7. Keywords: Luffa aegyptiaca; anti-proliferative effect; apoptosis; cell cycle; MCF-7 2016-12 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/61123/6/61123_Cancer%20Chemoprevention%20Study%20of%20Luffa%20Aegyptia_complete.pdf Hassan, Habibah and Razali, Shakirah and Firus Khan, Al'aina Yuhainis and K Jalal, Tara and Salleh, Azil and Khatib, Alfi and Hashim, Ridzwan and Abdul Wahab, Ridhwan (2016) Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7). In: 3rd Pan Asian Biomedical Science Conference 2016, 7-8th December 2016, Management and Science University (MSU), in Shah Alam, Selangor Darul Ehsan, Malaysia. (Unpublished) |
| spellingShingle | SF Animal culture Hassan, Habibah Razali, Shakirah Firus Khan, Al'aina Yuhainis K Jalal, Tara Salleh, Azil Khatib, Alfi Hashim, Ridzwan Abdul Wahab, Ridhwan Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) |
| title | Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) |
| title_full | Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) |
| title_fullStr | Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) |
| title_full_unstemmed | Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) |
| title_short | Cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (MCF-7) |
| title_sort | cancer chemoprevention study of luffa aegyptiaca (sponge gourd) seed extract on human breast cancer cell lines (mcf-7) |
| topic | SF Animal culture |
| url | http://irep.iium.edu.my/61123/ http://irep.iium.edu.my/61123/6/61123_Cancer%20Chemoprevention%20Study%20of%20Luffa%20Aegyptia_complete.pdf |