Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach
Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different so...
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| Format: | Proceeding Paper |
| Language: | English English |
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University Putra Malaysia
2017
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/61010/ http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf |
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| author | Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya |
| author_facet | Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya |
| author_sort | Perumal, Vikneswari |
| building | IIUM Repository |
| collection | Online Access |
| description | Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches.
Design and method: Initially, the fruit was extracted by soaking in different solvents with different concentrations of ethanol in water (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were tested for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. LC-MS based metabolomics approach correlated with multivariate data analysis was applied to profile the bioactive compounds present in the extract.
Results The 80% ethanol extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, LC-MS based metabolomics approaches helped to identify several antioxidants in this extract such as ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3-O-glycoside, kuguacin H, cucurbitacin E, 3-malonylmomordicin I, goyaglycoside G.
Conclusion: The results of the present study shows possible antioxidants from M. charantia fruit can be identified
Keywords: Momordica charantia, LCMS, Metabolomics, antioxidant, multivariate data analysis. |
| first_indexed | 2025-11-14T16:55:36Z |
| format | Proceeding Paper |
| id | iium-61010 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:55:36Z |
| publishDate | 2017 |
| publisher | University Putra Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-610102017-12-31T14:41:30Z http://irep.iium.edu.my/61010/ Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya QD Chemistry Objectives: The present study was design to identify the significant biomarkers from M.charantia that possessing antioxidant activity using liquid chromatography-mass spectrometry (LC-MS) based metabolomics approaches. Design and method: Initially, the fruit was extracted by soaking in different solvents with different concentrations of ethanol in water (0, 20, 40, 60, 80, 100%, v/v). Then, the extracts were tested for antioxidant activity using 1, 1-diphenyl-2 picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. LC-MS based metabolomics approach correlated with multivariate data analysis was applied to profile the bioactive compounds present in the extract. Results The 80% ethanol extract showed a high inhibitory activity on 1, 1-diphenyl-2 picrylhydrazyl (DPPH) radical and high ferric reducing antioxidant power, LC-MS based metabolomics approaches helped to identify several antioxidants in this extract such as ascorbic acid, margarolic acid, brevifolincarboxylic acid, quercetin 3-O-glycoside, kuguacin H, cucurbitacin E, 3-malonylmomordicin I, goyaglycoside G. Conclusion: The results of the present study shows possible antioxidants from M. charantia fruit can be identified Keywords: Momordica charantia, LCMS, Metabolomics, antioxidant, multivariate data analysis. University Putra Malaysia 2017-08-01 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf application/pdf en http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf Perumal, Vikneswari and Ahmed, Qamar Uddin and Khatib, Alfi and Uzir, Bisha Fathamah and Murugesu, Suganya (2017) Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach. In: 2nd International Anatomical and Biomedical Scientific Conference 2017, 1-2 Aug 2017, Serdang, Selangor. |
| spellingShingle | QD Chemistry Perumal, Vikneswari Ahmed, Qamar Uddin Khatib, Alfi Uzir, Bisha Fathamah Murugesu, Suganya Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
| title | Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
| title_full | Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
| title_fullStr | Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
| title_full_unstemmed | Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
| title_short | Evaluation of antioxidants activity of Momordica charantia using LC-MS metabolomics approach |
| title_sort | evaluation of antioxidants activity of momordica charantia using lc-ms metabolomics approach |
| topic | QD Chemistry |
| url | http://irep.iium.edu.my/61010/ http://irep.iium.edu.my/61010/1/IABS%202017%20PROCEEDINGS.pdf http://irep.iium.edu.my/61010/2/IABS%20-%20NESH-%20ABSTRACT.pdf |