Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism
Introduction: PAX9 (Paired box 9) gene is one of the genes which play significant role during craniofacial development. Single nucleotide polymorphism (SNP) in PAX9 has been associated with Class II/Division 2 malocclusion (with or without hypodontia). However, the relationship between PAX9 SNP mark...
| Main Authors: | , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia
2017
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/59807/ http://irep.iium.edu.my/59807/13/59807-Identification%20Of%20PAX9%20Single%20Nucleotide%20Polymorphism%20in%20Class%20III.pdf |
| _version_ | 1848785377966424064 |
|---|---|
| author | Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan |
| author_facet | Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan |
| author_sort | Abu Bakar, Noraini |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: PAX9 (Paired box 9) gene is one of the genes which play significant role during craniofacial development. Single nucleotide polymorphism (SNP) in PAX9 has been associated with Class II/Division 2 malocclusion (with or without hypodontia). However, the relationship between PAX9 SNP marker (rs8004560) with mandibular prognathism (MP) has not been analysed, at least in our local population. This study aimed to detect the presence of PAX9 (rs8004560) SNP in Class III malocclusion patients (with MP) in the local population. Materials and Methods: Genomic DNA were extracted from unstimulated saliva of 31 class I malocclusion (control samples) and 30 patients from Class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs8004560 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p<0.05). Results: Presence of PAX9 SNP (rs8004560) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within control (Class I) and Class III malocclusion. Conclusion(s): The most common allele of a marker flanking PAX9 (rs8004560) was over-represented in the mandibular prognathism (MP) subjects indicating the genetic association of PAX9 (rs8004560) SNP in the incidence of MP. Further studies involving larger number of samples should be developed in order to understand the exact role and mechanism of PAX9 in different classes of malocclusions. |
| first_indexed | 2025-11-14T16:52:11Z |
| format | Proceeding Paper |
| id | iium-59807 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:52:11Z |
| publishDate | 2017 |
| publisher | Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-598072018-07-10T04:45:36Z http://irep.iium.edu.my/59807/ Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan RK Dentistry Introduction: PAX9 (Paired box 9) gene is one of the genes which play significant role during craniofacial development. Single nucleotide polymorphism (SNP) in PAX9 has been associated with Class II/Division 2 malocclusion (with or without hypodontia). However, the relationship between PAX9 SNP marker (rs8004560) with mandibular prognathism (MP) has not been analysed, at least in our local population. This study aimed to detect the presence of PAX9 (rs8004560) SNP in Class III malocclusion patients (with MP) in the local population. Materials and Methods: Genomic DNA were extracted from unstimulated saliva of 31 class I malocclusion (control samples) and 30 patients from Class III malocclusion (MP). Cephalometric measurements were performed prior to saliva samples collection. The DNA was amplified using the specific primers for the marker rs8004560 and the genotyping was done by sequencing. Chi-square test was used to determine the over-representation of marker allele (p<0.05). Results: Presence of PAX9 SNP (rs8004560) was detected in local population analysed and the distribution of its genotype and allele could be observed. There were significant differences between allele (p=0.000) and genotype (p=0.000) frequency within control (Class I) and Class III malocclusion. Conclusion(s): The most common allele of a marker flanking PAX9 (rs8004560) was over-represented in the mandibular prognathism (MP) subjects indicating the genetic association of PAX9 (rs8004560) SNP in the incidence of MP. Further studies involving larger number of samples should be developed in order to understand the exact role and mechanism of PAX9 in different classes of malocclusions. Kulliyyah (Faculty) of Medicine, International Islamic University Malaysia 2017 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/59807/13/59807-Identification%20Of%20PAX9%20Single%20Nucleotide%20Polymorphism%20in%20Class%20III.pdf Abu Bakar, Noraini and Mokhtar@Makhtar, Khairani Idah and Kharuddin, Azrul Fazwan (2017) Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism. In: 1st IIUM International Dental Conference 2017 in Commemoration of the Kulliyyah of Dentistry 10th Anniversary (1st IIDC), 6th-8th October 2017, Kuantan, Pahang. http://iiumedic.net/imjm/v1/download/Volume%2016%20No%201%20Supp%202/ID56_IMJM-Vol16-Supp2.pdf |
| spellingShingle | RK Dentistry Abu Bakar, Noraini Mokhtar@Makhtar, Khairani Idah Kharuddin, Azrul Fazwan Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism |
| title | Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism |
| title_full | Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism |
| title_fullStr | Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism |
| title_full_unstemmed | Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism |
| title_short | Identification of PAX9 single nucleotide polymorphism in class III malocclusion patients with mandibular prognatism |
| title_sort | identification of pax9 single nucleotide polymorphism in class iii malocclusion patients with mandibular prognatism |
| topic | RK Dentistry |
| url | http://irep.iium.edu.my/59807/ http://irep.iium.edu.my/59807/ http://irep.iium.edu.my/59807/13/59807-Identification%20Of%20PAX9%20Single%20Nucleotide%20Polymorphism%20in%20Class%20III.pdf |