Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
The aim of this study is to develop a polymeric microcarrier (MC) for obtaining a higher cell density of Vero cells in spinner vessel. Different types of gelatin; bovine (BG), fish (FG), cationized bovine (CBG) and cationized fish (CFG) were covalently immobilized on carboxyl group containing po...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Malaysian Medical Associations
2010
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/5950/ http://irep.iium.edu.my/5950/1/Paper_MJM_2010-Yusilawati.pdf |
| _version_ | 1848776592440950784 |
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| author | Ahmad Nor, Yusilawati Mel, Maizirwan Mohd. Salleh, Hamzah Ng, Kim Hooi Wong, C. S. |
| author_facet | Ahmad Nor, Yusilawati Mel, Maizirwan Mohd. Salleh, Hamzah Ng, Kim Hooi Wong, C. S. |
| author_sort | Ahmad Nor, Yusilawati |
| building | IIUM Repository |
| collection | Online Access |
| description | The aim of this study is to develop a polymeric microcarrier (MC) for obtaining a higher cell density
of Vero cells in spinner vessel. Different types of
gelatin; bovine (BG), fish (FG), cationized bovine
(CBG) and cationized fish (CFG) were covalently
immobilized on carboxyl group containing polystyrene (COOH-PS). Their performance was evaluated by the cell loading test in Vero cell culture as carrier system. It was found that Vero cell has better performance on BG with highest maximum cell number at 1.45x105 cell/ml with good microscopic and suspension ability; easy sampling and easy cell recovery. |
| first_indexed | 2025-11-14T14:32:32Z |
| format | Article |
| id | iium-5950 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:32:32Z |
| publishDate | 2010 |
| publisher | Malaysian Medical Associations |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-59502021-07-06T01:53:35Z http://irep.iium.edu.my/5950/ Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture Ahmad Nor, Yusilawati Mel, Maizirwan Mohd. Salleh, Hamzah Ng, Kim Hooi Wong, C. S. TP248.13 Biotechnology The aim of this study is to develop a polymeric microcarrier (MC) for obtaining a higher cell density of Vero cells in spinner vessel. Different types of gelatin; bovine (BG), fish (FG), cationized bovine (CBG) and cationized fish (CFG) were covalently immobilized on carboxyl group containing polystyrene (COOH-PS). Their performance was evaluated by the cell loading test in Vero cell culture as carrier system. It was found that Vero cell has better performance on BG with highest maximum cell number at 1.45x105 cell/ml with good microscopic and suspension ability; easy sampling and easy cell recovery. Malaysian Medical Associations 2010 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5950/1/Paper_MJM_2010-Yusilawati.pdf Ahmad Nor, Yusilawati and Mel, Maizirwan and Mohd. Salleh, Hamzah and Ng, Kim Hooi and Wong, C. S. (2010) Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture. Medical Journal of Malaysia, 65. pp. 26-27. ISSN 0300-5283 |
| spellingShingle | TP248.13 Biotechnology Ahmad Nor, Yusilawati Mel, Maizirwan Mohd. Salleh, Hamzah Ng, Kim Hooi Wong, C. S. Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture |
| title | Study of immobilized bovine and fish gelatin on carboxyl
containing Polystyrene beads for Vero cell culture
|
| title_full | Study of immobilized bovine and fish gelatin on carboxyl
containing Polystyrene beads for Vero cell culture
|
| title_fullStr | Study of immobilized bovine and fish gelatin on carboxyl
containing Polystyrene beads for Vero cell culture
|
| title_full_unstemmed | Study of immobilized bovine and fish gelatin on carboxyl
containing Polystyrene beads for Vero cell culture
|
| title_short | Study of immobilized bovine and fish gelatin on carboxyl
containing Polystyrene beads for Vero cell culture
|
| title_sort | study of immobilized bovine and fish gelatin on carboxyl
containing polystyrene beads for vero cell culture |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5950/ http://irep.iium.edu.my/5950/1/Paper_MJM_2010-Yusilawati.pdf |