Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

QR Code

Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor

The purpose of this study was to optimize agitation speed, microcarrier concentration and pO2 levels in bioreactor for culturing DF-1cells in DMEM media supplemented with 7% fetal bovine serum. Statistical analysis has shown that the model derived from the results was proven to be significant and pO...

Full description

Bibliographic Details
Main Authors:	Mel, Maizirwan, Arifin, Mohd Azmir, Sohif, Hajar Naemah, Syed Hasan, Sharifah
Format:	Article
Language:	English
Published:	Malaysian Medical Associations 2010
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/5943/ http://irep.iium.edu.my/5943/1/Paper_MJM_Azmir.pdf

Similar Items

The growth study of DF1 cell in microcarrier based bioreactor
by: Arifin, Mohd Azmir, et al.
Published: (2011)

Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
by: Mel, Maizirwan, et al.
Published: (2010)

Optimization of process conditions for high cell density proliferation of DF-1 cells in bioreactor
by: Mel, Maizirwan, et al.
Published: (2011)

Cell attachment study of chicken fibroblast cell(DF-1) using ceramic microcarrier granule in bioreactors
by: Mel, Maizirwan, et al.
Published: (2008)

Modes of bioreactor operations
by: Mel, Maizirwan, et al.
Published: (2011)

Bioreactor for animals cells
by: Mel, Maizirwan, et al.
Published: (2011)

Microcarriers for cell culture technology
by: Mel, Maizirwan, et al.
Published: (2011)

Modeling the growth kinetics of Escherichia Coli fermentation in bioreactors
by: Ibrahim Ali , Noorbatcha, et al.
Published: (2011)

Cytotoxicity study of Schizophyllum commune extract towards DF-1 cell line
by: Abd rahman, Nur Rahilah, et al.
Published: (2012)

Virus culture : concept and procedures
by: Mel, Maizirwan, et al.
Published: (2011)

Ultraviolet/ozone treated polystyrene microcarriers for animal cell culture
by: Arifin, Mohd Azmir, et al.
Published: (2015)

The effect of Malaysian stingless bee, Trigona spp. honey in promoting proliferation of the undifferentiated stem cell
by: Mohamad, Mohd Amin Marwan, et al.
Published: (2019)

Vero cell culture on magnesium doped biphasic calcium phosphate scaffolds
by: Abd Rahim, Toibah, et al.
Published: (2016)

Monoclonal antibody production: media optimization for enhancement the cell viability of hybridoma cell
by: Mel, Maizirwan, et al.
Published: (2008)

Efficient performance of locally processed serum in vero cell culture
by: Ahmad Nor, Yusilawati, et al.
Published: (2011)

Cell disruption improvement of E. Coli producing NP-AIV using high pressure homogenizer
by: Mel, Maizirwan, et al.
Published: (2011)

Cell rupture of recombinant escherichia coli using high pressure homogenizer
by: Ahmad Raus, Raha, et al.
Published: (2010)

The growth study of vero cells in different type of microcarrier
by: Ahmad Nor, Yusilawati, et al.
Published: (2010)

Autologous human serum supports better proliferation, viability and homogeneity of peripheral blood mononuclear cells over fetal bovine serum
by: Haque, Nazmul, et al.
Published: (2014)

Mode of cell culture
by: Mel, Maizirwan, et al.
Published: (2011)

Optimization of process condition for E. Coli fermentation producing nucleocapsid protein-avian influenza virus (NP-AIV)
by: Mel, Maizirwan, et al.
Published: (2011)

Method for virus propagation in a bioreactor
by: Mel, Maizirwan, et al.
Published: (2012)

Study of immobilized bovine and fish gelatin on carboxyl containing Polystyrene beads for Vero cell culture
by: Ahmad Nor, Yusilawati, et al.
Published: (2010)

Development of semi-fluidized bed bioreactor as novel bioreactor system for the treatment of palm oil mill effluent (POME)
by: Alad, Abass O., et al.
Published: (2011)

A novel bioreactor system for the treatment of POME
by: Jameel, Ahmed Tariq, et al.
Published: (2011)

Optimization of process conditions for lactic acid recovery using organic base
by: Jamal, Parveen, et al.
Published: (2008)

Monoclonal antibody production: viability improvement of RC1 hybridoma cell in different types of bioreactor
by: Mel, Maizirwan, et al.
Published: (2008)

Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor
by: Mel, Maizirwan, et al.
Published: (2011)

Batch fermentation of recombinant Escherichia coli producing β-glucuronidase using different control condition
by: Abdul Karim, Mohamed Ismail, et al.
Published: (2011)

Bioprocessing of recombinant E.Coli producing Glucuronidase Enzymes
by: Mel, Maizirwan
Published: (2012)

Chinese hamster ovary (CHO-K1) cells expressed native insulin-like growth factor-1 (IGF-1) gene towards efficient mammalian cell culture host system
by: Packeer Mohamed, Vasila, et al.
Published: (2011)

Phases of cell culture
by: Arifin, Mohd Azmir, et al.
Published: (2011)

Different oxidation treatments on polystyrene (PS) microspheres by using an ultraviolet/ozone (UVO3) system
by: Mel, Maizirwan, et al.
Published: (2015)

Simple process capability analysis and quality validation of monoclonal antibody production in bench-top bioreactor
by: Mel, Maizirwan, et al.
Published: (2011)

CHO-K1 cells expressed IGF-1 Gene: a promising target towards optimal media at effective cost
by: Packeer Mohamed, Vasila, et al.
Published: (2011)

The effects of KGM, mannose and co-supplementation of KGM and mannose on mammalian cells cultured at inside and outside incubator conditions
by: Marzuke, Ainul Bashirah, et al.
Published: (2016)

Design and development of a lab scale bioreactor for heat inducible enzyme expression system
by: Adam, Daud, et al.
Published: (2011)

Effects of different types of growth media on metabolite profiles (GCMS-based) of CHO-K1 cells expressing IGF-1 proteins
by: Mohamed Saberi, Salfarina Ezrina, et al.
Published: (2011)

Comparative study of bioreactors used for palm oil mill effluent treatment based on chemical oxygen removal efficiencies
by: Jameel, Ahmed Tariq, et al.
Published: (2011)

Modification of Polycaprolactone microspheres surface by ultra violet/ozone treatment to improve its hydrophilicity
by: Samsudin, Nurhusna, et al.
Published: (2015)