Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions
The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six cent...
| Main Authors: | , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2006
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/5836/ http://irep.iium.edu.my/5836/1/BPE26_-_Md_Zahangir.pdf |
| _version_ | 1848776572464529408 |
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| author | Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Chowdhury, Ahmed Jalal Khan |
| author_facet | Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Chowdhury, Ahmed Jalal Khan |
| author_sort | Alam, Md. Zahangir |
| building | IIUM Repository |
| collection | Online Access |
| description | The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level
fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate
(wheat flour) concentrations, temperature, pH, inoculum and agitation. Statistical analysis showed that the optimum conditions were: temperature of 300C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm.
Under these conditions, the model predicted the enzyme production to be about 14 FPU/ml. Analysis of variance (ANOVA) of the design showed a high coefficient of determination (R2)value of 0.99, thus ensuring a high satisfactory adjustment of the quadratic model with the
experimental data. |
| first_indexed | 2025-11-14T14:32:13Z |
| format | Proceeding Paper |
| id | iium-5836 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:32:13Z |
| publishDate | 2006 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-58362024-12-24T07:48:14Z http://irep.iium.edu.my/5836/ Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Chowdhury, Ahmed Jalal Khan TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP Chemical technology TP248.13 Biotechnology The filamentous fungus Trichoderma harzianum was used for liquid state bioconversion of POME for cellulase production. Statistical optimization was carried out to evaluate the physico-chemical parameters (factors) for maximum cellulase production by 2-level fractional factorial design with six central points. The polynomial regression model was developed using the experimental data including the effects of linear, quadratic and interaction of the factors. The factors involved were substrate (POME) and co-substrate (wheat flour) concentrations, temperature, pH, inoculum and agitation. Statistical analysis showed that the optimum conditions were: temperature of 300C, substrate concentration of 2%, wheat flour concentration of 3%, pH of 4, inoculum of 3% and agitation of 200 rpm. Under these conditions, the model predicted the enzyme production to be about 14 FPU/ml. Analysis of variance (ANOVA) of the design showed a high coefficient of determination (R2)value of 0.99, thus ensuring a high satisfactory adjustment of the quadratic model with the experimental data. 2006-07-24 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/5836/1/BPE26_-_Md_Zahangir.pdf Alam, Md. Zahangir and Abdul Karim, Mohamed Ismail and Hamisan, Ahmad Fadhlan and Jamal, Parveen and Chowdhury, Ahmed Jalal Khan (2006) Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions. In: 1st International Conference on Natural Resources Engineering & Technology, 24-25 July 2006, PutraJaya. |
| spellingShingle | TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Abdul Karim, Mohamed Ismail Hamisan, Ahmad Fadhlan Jamal, Parveen Chowdhury, Ahmed Jalal Khan Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions |
| title | Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions |
| title_full | Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions |
| title_fullStr | Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions |
| title_full_unstemmed | Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions |
| title_short | Liquid State Bioconversion of Palm Oil Mill Effluent for Cellulase Production: Statistical Optimization of Process Conditions |
| title_sort | liquid state bioconversion of palm oil mill effluent for cellulase production: statistical optimization of process conditions |
| topic | TA164 Bioengineering TA170 Environmental engineering. Sustainable engineering TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5836/ http://irep.iium.edu.my/5836/1/BPE26_-_Md_Zahangir.pdf |