The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study
Dexamethasone is a glucocorticoid as a member of the steroidal anti-inflammatory and immunosuppressant. It has well documented effects on skeletal structures osseous and cartilaginous, commonly used to treat or control diseases. Objective To evaluate by histomorphometric study the effects of dexam...
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| Format: | Article |
| Language: | English |
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College of Medicine, Al-Nahrain University
2017
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| Online Access: | http://irep.iium.edu.my/58341/ http://irep.iium.edu.my/58341/1/The%20Effects%20of%20Dexamethasone%20on%20Tibia.pdf |
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| author | Ali, Wathiq Q.S. Selman, Mohammad Oda Al-Hasson, Indaf J. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. |
| author_facet | Ali, Wathiq Q.S. Selman, Mohammad Oda Al-Hasson, Indaf J. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. |
| author_sort | Ali, Wathiq Q.S. |
| building | IIUM Repository |
| collection | Online Access |
| description | Dexamethasone is a glucocorticoid as a member of the steroidal anti-inflammatory and immunosuppressant. It has well documented effects on skeletal structures osseous and cartilaginous, commonly used to treat or control diseases.
Objective
To evaluate by histomorphometric study the effects of dexamethasone on the embryogenesis of long bones in chick embryos.
Methods
Forty-eight fertile chick eggs of Gallus gallus domesticus, were used. The eggs were divided into 2 groups; control and treated groups of 24 eggs each, these groups were subdivided into 4 subgroups (n=6 eggs). On day 10 of incubation, the control group was injected with 25 μl of distilled water while the treated group was injected with 25 μl of distilled water contained 8 μg dexamethasone. In the next days (11, 12, 13, and 14 of incubation), 12 chick embryos were sacrificed in each day. A computer-assisted morphometric/ image analysis (Motic Image Plus version 2.0ML), was used to measure length, area, perimeter of tibiae, and the area and perimeter of the perichondral osseous collar of cross section in mid-diaphyseal zone of these bones.
Results
These bones of chick embryos treated with dexamethasone, suffered shortening and retardation in length, weight, area and perimeter throughout the period of this study, decline area and perimeter to the perichondral osseous collar in the mid-diaphyseal zone.
Conclusion
Dexamethasone given at day 10 of incubation caused tibial bones growth retardation at development stages 11, 12, 13, and 14-days; this was observed in the measured parameters: bone length, area, perimeter and weight. |
| first_indexed | 2025-11-14T16:47:41Z |
| format | Article |
| id | iium-58341 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:47:41Z |
| publishDate | 2017 |
| publisher | College of Medicine, Al-Nahrain University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-583412017-11-01T07:33:01Z http://irep.iium.edu.my/58341/ The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study Ali, Wathiq Q.S. Selman, Mohammad Oda Al-Hasson, Indaf J. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. QM Human anatomy QP Physiology RB Pathology Dexamethasone is a glucocorticoid as a member of the steroidal anti-inflammatory and immunosuppressant. It has well documented effects on skeletal structures osseous and cartilaginous, commonly used to treat or control diseases. Objective To evaluate by histomorphometric study the effects of dexamethasone on the embryogenesis of long bones in chick embryos. Methods Forty-eight fertile chick eggs of Gallus gallus domesticus, were used. The eggs were divided into 2 groups; control and treated groups of 24 eggs each, these groups were subdivided into 4 subgroups (n=6 eggs). On day 10 of incubation, the control group was injected with 25 μl of distilled water while the treated group was injected with 25 μl of distilled water contained 8 μg dexamethasone. In the next days (11, 12, 13, and 14 of incubation), 12 chick embryos were sacrificed in each day. A computer-assisted morphometric/ image analysis (Motic Image Plus version 2.0ML), was used to measure length, area, perimeter of tibiae, and the area and perimeter of the perichondral osseous collar of cross section in mid-diaphyseal zone of these bones. Results These bones of chick embryos treated with dexamethasone, suffered shortening and retardation in length, weight, area and perimeter throughout the period of this study, decline area and perimeter to the perichondral osseous collar in the mid-diaphyseal zone. Conclusion Dexamethasone given at day 10 of incubation caused tibial bones growth retardation at development stages 11, 12, 13, and 14-days; this was observed in the measured parameters: bone length, area, perimeter and weight. College of Medicine, Al-Nahrain University 2017-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58341/1/The%20Effects%20of%20Dexamethasone%20on%20Tibia.pdf Ali, Wathiq Q.S. and Selman, Mohammad Oda and Al-Hasson, Indaf J. and Al-Ani, Imad Matloub Dally and Al-Salihi, Anam R. (2017) The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study. Iraqi Journal of Medical Sciences, 15 (2). pp. 116-128. ISSN 0681-6579 E-ISSN 2224-4719 http://www.iraqijms.com/index.php?do=view&type=article&id=483 |
| spellingShingle | QM Human anatomy QP Physiology RB Pathology Ali, Wathiq Q.S. Selman, Mohammad Oda Al-Hasson, Indaf J. Al-Ani, Imad Matloub Dally Al-Salihi, Anam R. The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study |
| title | The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study |
| title_full | The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study |
| title_fullStr | The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study |
| title_full_unstemmed | The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study |
| title_short | The effects of dexamethasone on Tibia development of local chick-Embryo. I: computer-assisted morphometric study |
| title_sort | effects of dexamethasone on tibia development of local chick-embryo. i: computer-assisted morphometric study |
| topic | QM Human anatomy QP Physiology RB Pathology |
| url | http://irep.iium.edu.my/58341/ http://irep.iium.edu.my/58341/ http://irep.iium.edu.my/58341/1/The%20Effects%20of%20Dexamethasone%20on%20Tibia.pdf |