Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology
Protease is a versatile industrial enzymes that is applicable in various industries including the food sector. Eventhough most commercial proteases are from animal and microbial origin, they each carries certain limitations involving religious, ethical and dietary concerns. Therefore, the discovery...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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2017
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| Online Access: | http://irep.iium.edu.my/58306/ http://irep.iium.edu.my/58306/1/Muhammad%20Shahrain%20Poster%20%20iCast2017.pdf http://irep.iium.edu.my/58306/19/58306_Icast.pdf |
| _version_ | 1848785087828590592 |
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| author | Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi |
| author_facet | Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi |
| author_sort | Shuhaimen, Muhammad Shahrain |
| building | IIUM Repository |
| collection | Online Access |
| description | Protease is a versatile industrial enzymes that is applicable in various industries including the food sector. Eventhough most commercial proteases are from animal and microbial origin, they each carries certain limitations involving religious, ethical and dietary concerns. Therefore, the discovery of proteases from plant as an alternative source should be given attention. In this study cashew (Anacardium occidentale) fruit, known to have dermatitic and anthelmintic properties, was chosen as source of protease, and the effect of the extraction process on its protease activity is evaluated. The extracted crude enzyme
was optimized by using response surface methodology (RSM) using a central composite rotatable design (CCRD) with variables studied were pH (x1), CaCl2 (x2), mixing time (x3) and mass (x4). The quadratic model obtained from the results is significant (R2 = 0.9739). Based on the RSM model, the optimal extraction conditions were obtained at pH 6.42, 8.94 mM CaCl2, 5.62 min mixing time and 18.55 g sample mass that yields protease 6.251 unit/mL of crude extract. |
| first_indexed | 2025-11-14T16:47:34Z |
| format | Proceeding Paper |
| id | iium-58306 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:47:34Z |
| publishDate | 2017 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-583062017-09-12T01:35:49Z http://irep.iium.edu.my/58306/ Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi S Agriculture (General) Protease is a versatile industrial enzymes that is applicable in various industries including the food sector. Eventhough most commercial proteases are from animal and microbial origin, they each carries certain limitations involving religious, ethical and dietary concerns. Therefore, the discovery of proteases from plant as an alternative source should be given attention. In this study cashew (Anacardium occidentale) fruit, known to have dermatitic and anthelmintic properties, was chosen as source of protease, and the effect of the extraction process on its protease activity is evaluated. The extracted crude enzyme was optimized by using response surface methodology (RSM) using a central composite rotatable design (CCRD) with variables studied were pH (x1), CaCl2 (x2), mixing time (x3) and mass (x4). The quadratic model obtained from the results is significant (R2 = 0.9739). Based on the RSM model, the optimal extraction conditions were obtained at pH 6.42, 8.94 mM CaCl2, 5.62 min mixing time and 18.55 g sample mass that yields protease 6.251 unit/mL of crude extract. 2017-08-08 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/58306/1/Muhammad%20Shahrain%20Poster%20%20iCast2017.pdf application/pdf en http://irep.iium.edu.my/58306/19/58306_Icast.pdf Shuhaimen, Muhammad Shahrain and Omar, Muhammad Nor and Abdullah, Erna Normaya and Ahmad, Mohammad Norazmi (2017) Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology. In: 6th International Conference on Advancement in Science and Technology (iCAST 2017), 8th-9th August 2017, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/icast/?page_id=722 |
| spellingShingle | S Agriculture (General) Shuhaimen, Muhammad Shahrain Omar, Muhammad Nor Abdullah, Erna Normaya Ahmad, Mohammad Norazmi Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology |
| title | Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology |
| title_full | Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology |
| title_fullStr | Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology |
| title_full_unstemmed | Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology |
| title_short | Extraction of protease from Anacardium occidentale (Cashew) fruit using response surface methodology |
| title_sort | extraction of protease from anacardium occidentale (cashew) fruit using response surface methodology |
| topic | S Agriculture (General) |
| url | http://irep.iium.edu.my/58306/ http://irep.iium.edu.my/58306/ http://irep.iium.edu.my/58306/1/Muhammad%20Shahrain%20Poster%20%20iCast2017.pdf http://irep.iium.edu.my/58306/19/58306_Icast.pdf |