Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses
The aim of this study was to observe the effect of abiotic stress under light and dark conditions on pumpkin calluses carotenoid. Plant elicitors used to create abiotic stress in this study were Polyethylene Glycol 4000 for drought stress, Jasmonic Acid and Salicylic Acid for hormones stress and Mur...
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| Format: | Article |
| Language: | English |
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Journal of Fundamental and Applied Sciences
2017
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| Online Access: | http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/1/JFAS%201.pdf |
| _version_ | 1848785037245284352 |
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| author | Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun |
| author_facet | Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun |
| author_sort | Shahidan, Norshazila |
| building | IIUM Repository |
| collection | Online Access |
| description | The aim of this study was to observe the effect of abiotic stress under light and dark conditions on pumpkin calluses carotenoid. Plant elicitors used to create abiotic stress in this study were Polyethylene Glycol 4000 for drought stress, Jasmonic Acid and Salicylic Acid for hormones stress and Murashige and Skoog Salt for control and half strength nutrients. Carotneoid content was determined using High Performance Liquid Chromatography. Under light condition, the value of lutein was in range 0.554 + 0.107 µg/g to 0.169+ 0.003 µg/g and β-carotene was in range of not detected up to 8.133 + 0.088 µg/g. Differ to dark condition, a trace amount of lutein was detected in each treatments and the highest amount of β-carotene was detected in Polyethylene glycol treatment (5.313 + 0.051 µg/g). Carotenoid content varies in each treatment due to adaptation of pumpkin calluses with abiotic stress induced to them. |
| first_indexed | 2025-11-14T16:46:46Z |
| format | Article |
| id | iium-58018 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:46:46Z |
| publishDate | 2017 |
| publisher | Journal of Fundamental and Applied Sciences |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-580182018-03-20T02:46:48Z http://irep.iium.edu.my/58018/ Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology The aim of this study was to observe the effect of abiotic stress under light and dark conditions on pumpkin calluses carotenoid. Plant elicitors used to create abiotic stress in this study were Polyethylene Glycol 4000 for drought stress, Jasmonic Acid and Salicylic Acid for hormones stress and Murashige and Skoog Salt for control and half strength nutrients. Carotneoid content was determined using High Performance Liquid Chromatography. Under light condition, the value of lutein was in range 0.554 + 0.107 µg/g to 0.169+ 0.003 µg/g and β-carotene was in range of not detected up to 8.133 + 0.088 µg/g. Differ to dark condition, a trace amount of lutein was detected in each treatments and the highest amount of β-carotene was detected in Polyethylene glycol treatment (5.313 + 0.051 µg/g). Carotenoid content varies in each treatment due to adaptation of pumpkin calluses with abiotic stress induced to them. Journal of Fundamental and Applied Sciences 2017-08-08 Article PeerReviewed application/pdf en http://irep.iium.edu.my/58018/1/JFAS%201.pdf Shahidan, Norshazila and Othman, Rashidi and Jaswir, Irwandi and Hashim, Yumi Zuhanis Has-Yun (2017) Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses. Journal of Fundamental and Applied Sciences, 9 (2S (Special issue)). pp. 861-875. ISSN 1112-9867 http://www.jfas.info/index.php/jfas/article/view/2708/1348 10.4314/jfas.v9i2s.56 |
| spellingShingle | RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology Shahidan, Norshazila Othman, Rashidi Jaswir, Irwandi Hashim, Yumi Zuhanis Has-Yun Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses |
| title | Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses |
| title_full | Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses |
| title_fullStr | Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses |
| title_full_unstemmed | Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses |
| title_short | Effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (Cucurbita moshata) calluses |
| title_sort | effect of abiotic stress under light and dark conditions on carotenoid content in pumpkin (cucurbita moshata) calluses |
| topic | RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/ http://irep.iium.edu.my/58018/1/JFAS%201.pdf |