Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters

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Investigation on plasmid DNA separation under vacuum suction using cellulose generated and polyethersulfone ultra filters

E. coli DH5α harbouring pET28a+ plasmid vector was lysed by alkaline lysis and the clarified plasmid solutions were subjected to ultrafiltration experiments using two types of ultrafiltration membrane; i.e., cellulose generated (Ultracel) and polyethersulfome PES (Biomax). Transmembrane pressures...

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Bibliographic Details
Main Authors:	Tengku Abdul Hamid, Tengku Haziyamin, Abdul Hamid, Azzmer Azzar
Format:	Article
Language:	English English
Published:	Open science Publisher 2017
Subjects:	QR Microbiology
Online Access:	http://irep.iium.edu.my/57890/ http://irep.iium.edu.my/57890/1/Japs_Tg_Affaro.pdf http://irep.iium.edu.my/57890/7/57890_Investigation%20on%20plasmid%20DNA%20separation_SCOPUS.pdf

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