Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient
Qualitative and quantitative analysis of individual carotenoids content and composition are complicated, time consuming and in fact very costly. The crucial and vital part is the availability and reliability of the pure standards. Most of the individual carotenoids are commercially available either...
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| Language: | English English |
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Lifescience Global
2017
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| Online Access: | http://irep.iium.edu.my/57635/ http://irep.iium.edu.my/57635/1/JPANSV7N3A4-Rashidi.pdf http://irep.iium.edu.my/57635/7/Isolation%20and%20purification%20of%20%CE%B2-Carotene%20from%20Morinda%20citrifolia%20as%20HPLC%20standard%20and%20active%20pharmaceutical%20ingredient.pdf |
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| author | Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian |
| author_facet | Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian |
| author_sort | Othman, Rashidi |
| building | IIUM Repository |
| collection | Online Access |
| description | Qualitative and quantitative analysis of individual carotenoids content and composition are complicated, time consuming and in fact very costly. The crucial and vital part is the availability and reliability of the pure standards. Most of the individual carotenoids are commercially available either in natural or synthetic form but they are quite expensive and some of it not available in the market anymore. These problems strongly associated with the accuracy and reliability of High Performance Liquid Chromatography (HPLC) analysis data. Therefore, this study aimed to set up an analytical scheme of obtaining β-carotene standard from the leaves of Morinda citrifolia as one of the carotenoid standards for HPLC analysis. M. citrifolia has been selected due to its abundance throughout the year with tropical climate. The scheme via open column chromatography (OCC) established that the purity of β-carotene standard was 97% and the coefficient of correlation was 0.9923. However after 30 day storage period of time, the purity decreased to 95.46%. Although these had an effect on the carotenoid standard stability but it can be a reliable source of β-carotene standard for HPLC analysis as well as active pharmaceutical ingredient for cosmeceutical, nutraceutical, food and beverage industries. |
| first_indexed | 2025-11-14T16:45:42Z |
| format | Article |
| id | iium-57635 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:45:42Z |
| publishDate | 2017 |
| publisher | Lifescience Global |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-576352021-07-23T15:51:21Z http://irep.iium.edu.my/57635/ Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian QD Chemistry RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Qualitative and quantitative analysis of individual carotenoids content and composition are complicated, time consuming and in fact very costly. The crucial and vital part is the availability and reliability of the pure standards. Most of the individual carotenoids are commercially available either in natural or synthetic form but they are quite expensive and some of it not available in the market anymore. These problems strongly associated with the accuracy and reliability of High Performance Liquid Chromatography (HPLC) analysis data. Therefore, this study aimed to set up an analytical scheme of obtaining β-carotene standard from the leaves of Morinda citrifolia as one of the carotenoid standards for HPLC analysis. M. citrifolia has been selected due to its abundance throughout the year with tropical climate. The scheme via open column chromatography (OCC) established that the purity of β-carotene standard was 97% and the coefficient of correlation was 0.9923. However after 30 day storage period of time, the purity decreased to 95.46%. Although these had an effect on the carotenoid standard stability but it can be a reliable source of β-carotene standard for HPLC analysis as well as active pharmaceutical ingredient for cosmeceutical, nutraceutical, food and beverage industries. Lifescience Global 2017-07-14 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57635/1/JPANSV7N3A4-Rashidi.pdf application/pdf en http://irep.iium.edu.my/57635/7/Isolation%20and%20purification%20of%20%CE%B2-Carotene%20from%20Morinda%20citrifolia%20as%20HPLC%20standard%20and%20active%20pharmaceutical%20ingredient.pdf Othman, Rashidi and Mohd Zaifuddin, Fatimah Azzahra and Mohd Hassan, Norazian (2017) Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient. Journal of Pharmacy and Nutrition Sciences, 7 (3). pp. 95-99. ISSN 2223-3806 E-ISSN 1927-5951 http://www.lifescienceglobal.com/pms/index.php/jpans/article/view/4789 |
| spellingShingle | QD Chemistry RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Othman, Rashidi Mohd Zaifuddin, Fatimah Azzahra Mohd Hassan, Norazian Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient |
| title | Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient |
| title_full | Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient |
| title_fullStr | Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient |
| title_full_unstemmed | Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient |
| title_short | Isolation and purification of β-Carotene from Morinda citrifolia as HPLC standard and active pharmaceutical ingredient |
| title_sort | isolation and purification of β-carotene from morinda citrifolia as hplc standard and active pharmaceutical ingredient |
| topic | QD Chemistry RS Pharmacy and materia medica S Agriculture (General) SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc |
| url | http://irep.iium.edu.my/57635/ http://irep.iium.edu.my/57635/ http://irep.iium.edu.my/57635/1/JPANSV7N3A4-Rashidi.pdf http://irep.iium.edu.my/57635/7/Isolation%20and%20purification%20of%20%CE%B2-Carotene%20from%20Morinda%20citrifolia%20as%20HPLC%20standard%20and%20active%20pharmaceutical%20ingredient.pdf |