Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum
Calophyllum is a pan-tropical genus belongs to the Guttiferae family and known by Malay local folks as ‘bintangor’. There has been a continual interest to further investigate the phytochemicals of Calophyllum species since this genus is a rich source of active secondary metabolites such as coumarin,...
| Main Authors: | , , , |
|---|---|
| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2018
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/57430/ http://irep.iium.edu.my/57430/1/57430_Isolation%2C%20cytotoxic.pdf http://irep.iium.edu.my/57430/7/69556.pdf |
| _version_ | 1848784930518073344 |
|---|---|
| author | Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden |
| author_facet | Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden |
| author_sort | Aminudin, Nurul Iman |
| building | IIUM Repository |
| collection | Online Access |
| description | Calophyllum is a pan-tropical genus belongs to the Guttiferae family and known by Malay local folks as ‘bintangor’. There has been a continual interest to further investigate the phytochemicals of Calophyllum species since this genus is a rich source of active secondary metabolites such as coumarin, chromanone carboxylic acid and flavonoid which showed anti-HIV, cytotoxicity and antimicrobial properties. In this study, phytochemicals studies on the barks and leaves of Calophyllum incrassatum M.R. Henderson & Wyatt-Smith were carried out. Cold extraction method was employed by using dichloromethane, ethyl acetate and methanol to give the respective crude extracts. Four new 4-substituted coumarins, namely, incrassamarin A, B, C and D were successfully isolated from the dichloromethane extracts of the barks and leaves by size-exclusion recycling HPLC and column chromatography techniques. The structures of the compounds were elucidated by spectral analysis including NMR, IR, MS and X-ray crystallography. The compounds were tested for their cytotoxic activity towards human breast adenocarcinoma (MCF-7) and human non-small cell lung carcinoma (A-549) cell lines by MTT assay and α-glucosidase enzymatic inhibitory activity. Incrassamarin A displayed cytotoxic activity against A-549 cell lines with IC50 87.71 mg/ml and showed inhibition towards α-glucosidase enzymatic activity with IC50 93.25 mM. |
| first_indexed | 2025-11-14T16:45:04Z |
| format | Proceeding Paper |
| id | iium-57430 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:45:04Z |
| publishDate | 2018 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-574302019-01-30T04:24:23Z http://irep.iium.edu.my/57430/ Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden RS192 Materia Medica-Pharmaceutical Technology Calophyllum is a pan-tropical genus belongs to the Guttiferae family and known by Malay local folks as ‘bintangor’. There has been a continual interest to further investigate the phytochemicals of Calophyllum species since this genus is a rich source of active secondary metabolites such as coumarin, chromanone carboxylic acid and flavonoid which showed anti-HIV, cytotoxicity and antimicrobial properties. In this study, phytochemicals studies on the barks and leaves of Calophyllum incrassatum M.R. Henderson & Wyatt-Smith were carried out. Cold extraction method was employed by using dichloromethane, ethyl acetate and methanol to give the respective crude extracts. Four new 4-substituted coumarins, namely, incrassamarin A, B, C and D were successfully isolated from the dichloromethane extracts of the barks and leaves by size-exclusion recycling HPLC and column chromatography techniques. The structures of the compounds were elucidated by spectral analysis including NMR, IR, MS and X-ray crystallography. The compounds were tested for their cytotoxic activity towards human breast adenocarcinoma (MCF-7) and human non-small cell lung carcinoma (A-549) cell lines by MTT assay and α-glucosidase enzymatic inhibitory activity. Incrassamarin A displayed cytotoxic activity against A-549 cell lines with IC50 87.71 mg/ml and showed inhibition towards α-glucosidase enzymatic activity with IC50 93.25 mM. 2018 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/57430/1/57430_Isolation%2C%20cytotoxic.pdf application/pdf en http://irep.iium.edu.my/57430/7/69556.pdf Aminudin, Nurul Iman and Ahmad, Farediah and Bakhtiar, M. Taher and Mohamed Zulkifli, Razauden (2018) Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum. In: 34th International Conference on Natural Products 2018 (ICNP 2018), 19th-21st March 2018, Penang, Malaysia. (Unpublished) http://www.icnp2018.usm.my/ |
| spellingShingle | RS192 Materia Medica-Pharmaceutical Technology Aminudin, Nurul Iman Ahmad, Farediah Bakhtiar, M. Taher Mohamed Zulkifli, Razauden Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum |
| title | Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum |
| title_full | Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum |
| title_fullStr | Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum |
| title_full_unstemmed | Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum |
| title_short | Isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum |
| title_sort | isolation, cytotoxic and alpha-glucosidase inhibitory activities of new coumarins from calophyllum incrassatum |
| topic | RS192 Materia Medica-Pharmaceutical Technology |
| url | http://irep.iium.edu.my/57430/ http://irep.iium.edu.my/57430/ http://irep.iium.edu.my/57430/1/57430_Isolation%2C%20cytotoxic.pdf http://irep.iium.edu.my/57430/7/69556.pdf |