Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study
Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence. Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular complication in diabetes. In this research, we developed and validated a high thr...
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| Format: | Article |
| Language: | English English |
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Xi'an Jiaotong University
2017
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| Online Access: | http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/12/57104_Development%20and%20validation%20of%20a%20high%20throughput_article.pdf http://irep.iium.edu.my/57104/7/A%20high%20throughput%20LC-MS%20MS%20method%20for%20simultaneous%20quantitation%20of%20pioglitazone%20and%20telmisartan%20in%20rat%20plasma%20development%2C%20validation%20and%20pharmacokinetic%20application.pdf |
| _version_ | 1848784866538160128 |
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| author | Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar |
| author_facet | Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar |
| author_sort | Sengupta, Pinaki |
| building | IIUM Repository |
| collection | Online Access |
| description | Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence.
Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular
complication in diabetes. In this research, we developed and validated a high throughput LC–MS/MS method for
simultaneous quantitation of PIO and TLM in rat plasma. This developed method is more sensitive and can
quantitate the analytes in relatively shorter period of time compared to the previously reported methods for their
individual quantification. Moreover, till date, there is no bioanalytical method available to simultaneously
quantitate PIO and TLM in a single run. The method was validated according to the USFDA guidelines for
bioanalytical method validation. A linear response of the analytes was observed over the range of 0.005–10 µg/mL
with satisfactory precision and accuracy. Accuracy at four quality control levels was within 94.27%–106.10%.
The intra- and inter-day precision ranged from 2.32% to 10.14% and 5.02% to 8.12%, respectively. The method
was reproducible and sensitive enough to quantitate PIO and TLM in rat plasma samples of a preclinical
pharmacokinetic study. Due to the potential of PIO-TLM combination to be therapeutically explored, this method
is expected to have significant usefulness in future. |
| first_indexed | 2025-11-14T16:44:03Z |
| format | Article |
| id | iium-57104 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:44:03Z |
| publishDate | 2017 |
| publisher | Xi'an Jiaotong University |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-571042018-04-18T01:43:18Z http://irep.iium.edu.my/57104/ Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar RS Pharmacy and materia medica Management of cardiovascular risk factors in diabetes demands special attention due to their co-existence. Pioglitazone (PIO) and telmisartan (TLM) combination can be beneficial in effective control of cardiovascular complication in diabetes. In this research, we developed and validated a high throughput LC–MS/MS method for simultaneous quantitation of PIO and TLM in rat plasma. This developed method is more sensitive and can quantitate the analytes in relatively shorter period of time compared to the previously reported methods for their individual quantification. Moreover, till date, there is no bioanalytical method available to simultaneously quantitate PIO and TLM in a single run. The method was validated according to the USFDA guidelines for bioanalytical method validation. A linear response of the analytes was observed over the range of 0.005–10 µg/mL with satisfactory precision and accuracy. Accuracy at four quality control levels was within 94.27%–106.10%. The intra- and inter-day precision ranged from 2.32% to 10.14% and 5.02% to 8.12%, respectively. The method was reproducible and sensitive enough to quantitate PIO and TLM in rat plasma samples of a preclinical pharmacokinetic study. Due to the potential of PIO-TLM combination to be therapeutically explored, this method is expected to have significant usefulness in future. Xi'an Jiaotong University 2017-05-18 Article PeerReviewed application/pdf en http://irep.iium.edu.my/57104/12/57104_Development%20and%20validation%20of%20a%20high%20throughput_article.pdf application/pdf en http://irep.iium.edu.my/57104/7/A%20high%20throughput%20LC-MS%20MS%20method%20for%20simultaneous%20quantitation%20of%20pioglitazone%20and%20telmisartan%20in%20rat%20plasma%20development%2C%20validation%20and%20pharmacokinetic%20application.pdf Sengupta, Pinaki and Chatterjee, Bappaditya and Mandal, Uttam Kumar and Gorain, Bapi and Pal, Tapan Kumar (2017) Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study. Journal of Pharmaceutical Analysis, 7 (6). pp. 381-387. ISSN 2095-1779 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5790748/pdf/main.pdf 10.1016/j.jpha.2017.05.004 |
| spellingShingle | RS Pharmacy and materia medica Sengupta, Pinaki Chatterjee, Bappaditya Mandal, Uttam Kumar Gorain, Bapi Pal, Tapan Kumar Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study |
| title | Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study |
| title_full | Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study |
| title_fullStr | Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study |
| title_full_unstemmed | Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study |
| title_short | Development and validation of a high throughput LC–MS/MS method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study |
| title_sort | development and validation of a high throughput lc–ms/ms method for simultaneous quantitation of pioglitazone and telmisartan in rat plasma and its application to a pharmacokinetic study |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/ http://irep.iium.edu.my/57104/12/57104_Development%20and%20validation%20of%20a%20high%20throughput_article.pdf http://irep.iium.edu.my/57104/7/A%20high%20throughput%20LC-MS%20MS%20method%20for%20simultaneous%20quantitation%20of%20pioglitazone%20and%20telmisartan%20in%20rat%20plasma%20development%2C%20validation%20and%20pharmacokinetic%20application.pdf |