The simultaneous analysis of eight essential trace elements in human milk by ICP-MS
Trace elements are commonly present as components of metabolic enzymes, hormones and antioxidants in human milk. Previous studies have reported single or few elements in relatively large volumes of human milk using complex, time-consuming and expensive methods involving microwave-assisted acid...
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| Language: | English English English |
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Springer New York
2016
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| Online Access: | http://irep.iium.edu.my/56676/ http://irep.iium.edu.my/56676/1/The%20simultaneous%20analysis%20of%208%20essential%20trace%20elements%20in%20human%20breast%20milkby%20ICPMS.pdf http://irep.iium.edu.my/56676/7/56676_The%20Simultaneous%20Analysis_SCOPUS.pdf http://irep.iium.edu.my/56676/8/56676_The%20Simultaneous%20Analysis_WOS.pdf |
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| author | Mohd Taufek, Nor Hidayah Cartwright, David William Davies, Mark W. Hewavitharana, Amitha K Koorts, Pieter J. Shaw, Paul Nicholas Sumner, Ronald Lee, Eugene Whitfield, Karen M. |
| author_facet | Mohd Taufek, Nor Hidayah Cartwright, David William Davies, Mark W. Hewavitharana, Amitha K Koorts, Pieter J. Shaw, Paul Nicholas Sumner, Ronald Lee, Eugene Whitfield, Karen M. |
| author_sort | Mohd Taufek, Nor Hidayah |
| building | IIUM Repository |
| collection | Online Access |
| description | Trace elements are commonly present as components
of metabolic enzymes, hormones and antioxidants in
human milk. Previous studies have reported single or few
elements in relatively large volumes of human milk using
complex, time-consuming and expensive methods involving
microwave-assisted acid digestion and extraction using
tetramethylammonium hydroxide at various temperatures.
We report here a validated alkaline dissolution method using ethylenediaminetetraacetic acid, ammonia solution,
isopropanol and Triton X-100 to simultaneously determine
trace elements in 0.2 mL samples of human milk by inductively coupled plasma mass spectrometry (ICP-MS). The trace elements zinc (Zn), copper (Cu), selenium (Se), manganese (Mn), iodine (I), iron (Fe), molybdenum (Mo), bromine (Br), chromium (Cr), cobalt (Co), lead (Pb), nickel (Ni), silver (Ag), cadmium (Cd), arsenic (As), bismuth (Bi), aluminium (Al),antimony (Sb), vanadium (V), thallium (Tl) and uranium (U)were detected, and the method was applied quantitatively to 12 samples of human milk. The results for method validation showed good sensitivity, accuracy and repeatability for Zn,
Cu, Se, Mn, I, Fe, Mo and Br. The mean±SD of these
elements in the above human milk samples (μg/L) were
1390.6 ± 211.5, 220.8 ± 32.9, 14.3 ± 5.8, 1.37 ± 0.14, 113.5 ± 17.1, 47.3± 99.9, 0.37± 0.12 and 812.6 ± 127.7, respectively. This method is precise, reliable, straightforward and cost effective in the determination of trace elements simultaneously in small sample volumes of human milk. Method application permits routine monitoring of several elements and the ongoing assessment of trace element nutrition in breast milk. It is the first method to highlight the relatively high Br levels
present in human milk |
| first_indexed | 2025-11-14T16:42:47Z |
| format | Article |
| id | iium-56676 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T16:42:47Z |
| publishDate | 2016 |
| publisher | Springer New York |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-566762017-10-23T04:44:38Z http://irep.iium.edu.my/56676/ The simultaneous analysis of eight essential trace elements in human milk by ICP-MS Mohd Taufek, Nor Hidayah Cartwright, David William Davies, Mark W. Hewavitharana, Amitha K Koorts, Pieter J. Shaw, Paul Nicholas Sumner, Ronald Lee, Eugene Whitfield, Karen M. RS Pharmacy and materia medica Trace elements are commonly present as components of metabolic enzymes, hormones and antioxidants in human milk. Previous studies have reported single or few elements in relatively large volumes of human milk using complex, time-consuming and expensive methods involving microwave-assisted acid digestion and extraction using tetramethylammonium hydroxide at various temperatures. We report here a validated alkaline dissolution method using ethylenediaminetetraacetic acid, ammonia solution, isopropanol and Triton X-100 to simultaneously determine trace elements in 0.2 mL samples of human milk by inductively coupled plasma mass spectrometry (ICP-MS). The trace elements zinc (Zn), copper (Cu), selenium (Se), manganese (Mn), iodine (I), iron (Fe), molybdenum (Mo), bromine (Br), chromium (Cr), cobalt (Co), lead (Pb), nickel (Ni), silver (Ag), cadmium (Cd), arsenic (As), bismuth (Bi), aluminium (Al),antimony (Sb), vanadium (V), thallium (Tl) and uranium (U)were detected, and the method was applied quantitatively to 12 samples of human milk. The results for method validation showed good sensitivity, accuracy and repeatability for Zn, Cu, Se, Mn, I, Fe, Mo and Br. The mean±SD of these elements in the above human milk samples (μg/L) were 1390.6 ± 211.5, 220.8 ± 32.9, 14.3 ± 5.8, 1.37 ± 0.14, 113.5 ± 17.1, 47.3± 99.9, 0.37± 0.12 and 812.6 ± 127.7, respectively. This method is precise, reliable, straightforward and cost effective in the determination of trace elements simultaneously in small sample volumes of human milk. Method application permits routine monitoring of several elements and the ongoing assessment of trace element nutrition in breast milk. It is the first method to highlight the relatively high Br levels present in human milk Springer New York 2016-07 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56676/1/The%20simultaneous%20analysis%20of%208%20essential%20trace%20elements%20in%20human%20breast%20milkby%20ICPMS.pdf application/pdf en http://irep.iium.edu.my/56676/7/56676_The%20Simultaneous%20Analysis_SCOPUS.pdf application/pdf en http://irep.iium.edu.my/56676/8/56676_The%20Simultaneous%20Analysis_WOS.pdf Mohd Taufek, Nor Hidayah and Cartwright, David William and Davies, Mark W. and Hewavitharana, Amitha K and Koorts, Pieter J. and Shaw, Paul Nicholas and Sumner, Ronald and Lee, Eugene and Whitfield, Karen M. (2016) The simultaneous analysis of eight essential trace elements in human milk by ICP-MS. Food Analytical Methods, 9 (7). pp. 2068-2075. ISSN 1936-9751 E-ISSN 1936-976X https://link.springer.com/article/10.1007%2Fs12161-015-0396-z 10.1007/s12161-015-0396-z |
| spellingShingle | RS Pharmacy and materia medica Mohd Taufek, Nor Hidayah Cartwright, David William Davies, Mark W. Hewavitharana, Amitha K Koorts, Pieter J. Shaw, Paul Nicholas Sumner, Ronald Lee, Eugene Whitfield, Karen M. The simultaneous analysis of eight essential trace elements in human milk by ICP-MS |
| title | The simultaneous analysis of eight essential trace elements
in human milk by ICP-MS |
| title_full | The simultaneous analysis of eight essential trace elements
in human milk by ICP-MS |
| title_fullStr | The simultaneous analysis of eight essential trace elements
in human milk by ICP-MS |
| title_full_unstemmed | The simultaneous analysis of eight essential trace elements
in human milk by ICP-MS |
| title_short | The simultaneous analysis of eight essential trace elements
in human milk by ICP-MS |
| title_sort | simultaneous analysis of eight essential trace elements
in human milk by icp-ms |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/56676/ http://irep.iium.edu.my/56676/ http://irep.iium.edu.my/56676/ http://irep.iium.edu.my/56676/1/The%20simultaneous%20analysis%20of%208%20essential%20trace%20elements%20in%20human%20breast%20milkby%20ICPMS.pdf http://irep.iium.edu.my/56676/7/56676_The%20Simultaneous%20Analysis_SCOPUS.pdf http://irep.iium.edu.my/56676/8/56676_The%20Simultaneous%20Analysis_WOS.pdf |