Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study
Leptin (LEP) and leptin receptor (LEPR) have long been found associated with breast cancer. So far no high-resolution method such as electron microscopy has been used to investigate the subcellular localization of leptin and leptin receptor in breast cancer. We collected cancer and non-cancer breast...
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Elsevier B.V.
2016
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| Online Access: | http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/19/Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor%20in%20breast%20cancer%20detected%20in%20an%20electron%20microscopic%20study.pdf http://irep.iium.edu.my/56560/2/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_Scopus.pdf http://irep.iium.edu.my/56560/3/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_WoS.pdf |
| _version_ | 1848784762201702400 |
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| author | Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh |
| author_facet | Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh |
| author_sort | Abdulrazzaq, Saad Mohammed |
| building | IIUM Repository |
| collection | Online Access |
| description | Leptin (LEP) and leptin receptor (LEPR) have long been found associated with breast cancer. So far no high-resolution method such as electron microscopy has been used to investigate the subcellular localization of leptin and leptin receptor in breast cancer. We collected cancer and non-cancer breast tissues from 51 women with invasive ductal breast cancer. Leptin and leptin receptor in the tissues were estimated using immunohistochemistry (IHC). LEP and LEPR were localized at subcellular level by immunocytochemistry (ICC) using ultra-fine gold particle conjugated antibody, and visualized with transmission electron microscopy (TEM). IHC showed high presence of LEP and LEPR in 65% and 67% respectively of the breast cancer samples, 100% and 0% respectively of the adipose tissue samples, and no high presence in the non-cancer breast tissue samples. On TEM views both LEP and LEPR were found highly concentrated within the nucleus of the cancer cells, indicating that nucleus is the principal seat of action. However, presence of high concentration of LEP does not necessarily prove its over-expression, as often concluded, because LEP could be internalized from outside by LEPR in the cells. In contrast, LEPR is definitely over-expressed in the ductal breast cancer cells. Therefore, we hypothesize that over-expression of LEPR, rather than that of LEP has a fundamental role in breast carcinogenesis in particular, and probably for LEP-LEPR associated tumors in general. |
| first_indexed | 2025-11-14T16:42:24Z |
| format | Article |
| id | iium-56560 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T16:42:24Z |
| publishDate | 2016 |
| publisher | Elsevier B.V. |
| recordtype | eprints |
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| spelling | iium-565602018-10-18T02:07:53Z http://irep.iium.edu.my/56560/ Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh RC254 Neoplasms. Tumors. Oncology (including Cancer) Leptin (LEP) and leptin receptor (LEPR) have long been found associated with breast cancer. So far no high-resolution method such as electron microscopy has been used to investigate the subcellular localization of leptin and leptin receptor in breast cancer. We collected cancer and non-cancer breast tissues from 51 women with invasive ductal breast cancer. Leptin and leptin receptor in the tissues were estimated using immunohistochemistry (IHC). LEP and LEPR were localized at subcellular level by immunocytochemistry (ICC) using ultra-fine gold particle conjugated antibody, and visualized with transmission electron microscopy (TEM). IHC showed high presence of LEP and LEPR in 65% and 67% respectively of the breast cancer samples, 100% and 0% respectively of the adipose tissue samples, and no high presence in the non-cancer breast tissue samples. On TEM views both LEP and LEPR were found highly concentrated within the nucleus of the cancer cells, indicating that nucleus is the principal seat of action. However, presence of high concentration of LEP does not necessarily prove its over-expression, as often concluded, because LEP could be internalized from outside by LEPR in the cells. In contrast, LEPR is definitely over-expressed in the ductal breast cancer cells. Therefore, we hypothesize that over-expression of LEPR, rather than that of LEP has a fundamental role in breast carcinogenesis in particular, and probably for LEP-LEPR associated tumors in general. Elsevier B.V. 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56560/19/Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor%20in%20breast%20cancer%20detected%20in%20an%20electron%20microscopic%20study.pdf application/pdf en http://irep.iium.edu.my/56560/2/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_Scopus.pdf application/pdf en http://irep.iium.edu.my/56560/3/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_WoS.pdf Abdulrazzaq, Saad Mohammed and Amjad, Nasser Muhammad and Al-Kubaisi, Muna Khaleel Dheyab and Mizan, Shaikh (2016) Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study. Biochemical and Biophysical Research Communications, 482 (4). pp. 1102-1106. ISSN 0006-291X E-ISSN 1090-2104 http://www.sciencedirect.com/science/article/pii/S0006291X16320393 http://doi.org/10.1016/j.bbrc.2016.11.165 |
| spellingShingle | RC254 Neoplasms. Tumors. Oncology (including Cancer) Abdulrazzaq, Saad Mohammed Amjad, Nasser Muhammad Al-Kubaisi, Muna Khaleel Dheyab Mizan, Shaikh Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study |
| title | Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study |
| title_full | Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study |
| title_fullStr | Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study |
| title_full_unstemmed | Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study |
| title_short | Subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study |
| title_sort | subcellular localization of leptin and leptin receptor in breast cancer detected in an electron microscopic study |
| topic | RC254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/ http://irep.iium.edu.my/56560/19/Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor%20in%20breast%20cancer%20detected%20in%20an%20electron%20microscopic%20study.pdf http://irep.iium.edu.my/56560/2/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_Scopus.pdf http://irep.iium.edu.my/56560/3/56560_Subcellular%20localization%20of%20leptin%20and%20leptin%20receptor_WoS.pdf |