Process optimization in citric acid production using sewage sludge as fermentation media
A research has been conducted on liquid state bioconversion for the production of citric acid using Sewage Treatment Plant (STP) sludge as main substrate and added with sucrose as co-substrate. Three parameters were examined as to determine the best process optimization conducted in two liter (2L) b...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2011
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| Online Access: | http://irep.iium.edu.my/5652/ http://irep.iium.edu.my/5652/1/ICBioE_2011_Hanapi_et_al.pdf |
| _version_ | 1848776542400806912 |
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| author | Mohamad Hanapi, Nur Hanis Alam, Md. Zahangir Abdulkarim, Mohd Ismail |
| author_facet | Mohamad Hanapi, Nur Hanis Alam, Md. Zahangir Abdulkarim, Mohd Ismail |
| author_sort | Mohamad Hanapi, Nur Hanis |
| building | IIUM Repository |
| collection | Online Access |
| description | A research has been conducted on liquid state bioconversion for the production of citric acid using Sewage Treatment Plant (STP) sludge as main substrate and added with sucrose as co-substrate. Three parameters were examined as to determine the best process optimization conducted in two liter (2L) bioreactor. Two level factorial designs with three parameters are applied for the fermentation. It was carried out using local isolates Aspergillus niger strain O103A at room temperature (30-32oC), agitation is set at three different values which are 100, 200 and 300rpm. Initial pH for fermentation is set at 5 and it was further adjusted to three levels which are 2, 3, and 4 on the second day of the fermentation. Another parameter is aeration where 1, 1.5 and 2 vvm were introduced. The fermentation was carried out for five days. The maximum yield of citric acid obtained throughout the fermentation is 23.68 g/L, which is due to low pH, low agitation and high aeration values. |
| first_indexed | 2025-11-14T14:31:45Z |
| format | Proceeding Paper |
| id | iium-5652 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:31:45Z |
| publishDate | 2011 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-56522011-11-21T23:45:47Z http://irep.iium.edu.my/5652/ Process optimization in citric acid production using sewage sludge as fermentation media Mohamad Hanapi, Nur Hanis Alam, Md. Zahangir Abdulkarim, Mohd Ismail TP Chemical technology TP248.13 Biotechnology A research has been conducted on liquid state bioconversion for the production of citric acid using Sewage Treatment Plant (STP) sludge as main substrate and added with sucrose as co-substrate. Three parameters were examined as to determine the best process optimization conducted in two liter (2L) bioreactor. Two level factorial designs with three parameters are applied for the fermentation. It was carried out using local isolates Aspergillus niger strain O103A at room temperature (30-32oC), agitation is set at three different values which are 100, 200 and 300rpm. Initial pH for fermentation is set at 5 and it was further adjusted to three levels which are 2, 3, and 4 on the second day of the fermentation. Another parameter is aeration where 1, 1.5 and 2 vvm were introduced. The fermentation was carried out for five days. The maximum yield of citric acid obtained throughout the fermentation is 23.68 g/L, which is due to low pH, low agitation and high aeration values. 2011-05-17 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/5652/1/ICBioE_2011_Hanapi_et_al.pdf Mohamad Hanapi, Nur Hanis and Alam, Md. Zahangir and Abdulkarim, Mohd Ismail (2011) Process optimization in citric acid production using sewage sludge as fermentation media. In: 2nd International Conference on Biotechnology Engineering (ICBioE 2011), 17-19 May 2011, The Legend Hotel, Kuala Lumpur. http://www.iium.edu.my/icbioe/2011/ |
| spellingShingle | TP Chemical technology TP248.13 Biotechnology Mohamad Hanapi, Nur Hanis Alam, Md. Zahangir Abdulkarim, Mohd Ismail Process optimization in citric acid production using sewage sludge as fermentation media |
| title | Process optimization in citric acid production using sewage sludge as fermentation media |
| title_full | Process optimization in citric acid production using sewage sludge as fermentation media |
| title_fullStr | Process optimization in citric acid production using sewage sludge as fermentation media |
| title_full_unstemmed | Process optimization in citric acid production using sewage sludge as fermentation media |
| title_short | Process optimization in citric acid production using sewage sludge as fermentation media |
| title_sort | process optimization in citric acid production using sewage sludge as fermentation media |
| topic | TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5652/ http://irep.iium.edu.my/5652/ http://irep.iium.edu.my/5652/1/ICBioE_2011_Hanapi_et_al.pdf |