Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system
Purpose Ionic liquids (ILs) have been found to be highly promising for lignocellulosic biomass pretreatment, due to their excellent abilities to dissolve biopolymers. However,they have found to inactivate enzymes. In previous studies,Cholinium-based ILs showed great compatibility with cellulases an...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Springer
2017
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/56454/ http://irep.iium.edu.my/56454/1/Implementation%20of%20Definite%20Screening%20Design%20in%20Optimization%20of%20in%20Situ.pdf http://irep.iium.edu.my/56454/8/56454-Implementation%20of%20definite%20screening%20design%20in%20optimization%20of%20in%20situ%20hydrolysis%20of%20EFB%20in%20cholinium%20acetate%20and%20locally%20produced%20cellulase%20combined%20system_SCOPUS.pdf |
| _version_ | 1848784742714966016 |
|---|---|
| author | Elgharbawy, Amal A. Alam, Md Zahangir Kabbashi, Nassereldeen Ahmed Moniruzzaman, Muhammad Jamal, Parveen |
| author_facet | Elgharbawy, Amal A. Alam, Md Zahangir Kabbashi, Nassereldeen Ahmed Moniruzzaman, Muhammad Jamal, Parveen |
| author_sort | Elgharbawy, Amal A. |
| building | IIUM Repository |
| collection | Online Access |
| description | Purpose Ionic liquids (ILs) have been found to be highly
promising for lignocellulosic biomass pretreatment, due to their excellent abilities to dissolve biopolymers. However,they have found to inactivate enzymes. In previous studies,Cholinium-based ILs showed great compatibility with cellulases and thus were introduced for a combined-system.
This study aims to find the optimum condition in order to
achieve the maximum pretreatment-hydrolysis of empty
fruit bunches (EFB). Methods Definitive screening design (DSD) was employed with seven factors that are thought to impact the process including pretreatment temperature, pretreatment time, hydrolysis time, enzyme loading, particle size, biomass loading, and IL/buffer ratio. DSD offered several solutions for optimization in which they were experimentally tested.
Results The maximum sugar concentration (77 g/L) was
obtained at 90 C, 70–116 min of treatment, 36–42 h of
hydrolysis, 40–62 Unit/g cellulase loading, 34–35 %, w/v)
biomass loading, 220–450 l particle size, and 10 % (v/v)
IL to buffer ratio, respectively. The subsequent optimization by response surface methodology (RSM) revealed the temperature for treatment can drop to 75 C while fixing the pretreatment time at 100 min. Around 75 unit/g of cellulase and [22.0 % (w/v) of the biomass could be loaded to achieve a minimum of 70.0 ± 7.83 g/L of sugar, equivalent to 0.38 ± 0.08 g glucose/g and 0.48 ± 0.05 g total reducing sugar/g dry EFB.
Conclusions The locally produced cellulase (PKC-Cel)
from Trichoderma reesei exhibited promising results
in the single-step process and can be used as an efficient approach to be optimized for fermentation to bioethanol production. |
| first_indexed | 2025-11-14T16:42:05Z |
| format | Article |
| id | iium-56454 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:42:05Z |
| publishDate | 2017 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-564542017-08-10T03:39:23Z http://irep.iium.edu.my/56454/ Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system Elgharbawy, Amal A. Alam, Md Zahangir Kabbashi, Nassereldeen Ahmed Moniruzzaman, Muhammad Jamal, Parveen TP155 Chemical engineering Purpose Ionic liquids (ILs) have been found to be highly promising for lignocellulosic biomass pretreatment, due to their excellent abilities to dissolve biopolymers. However,they have found to inactivate enzymes. In previous studies,Cholinium-based ILs showed great compatibility with cellulases and thus were introduced for a combined-system. This study aims to find the optimum condition in order to achieve the maximum pretreatment-hydrolysis of empty fruit bunches (EFB). Methods Definitive screening design (DSD) was employed with seven factors that are thought to impact the process including pretreatment temperature, pretreatment time, hydrolysis time, enzyme loading, particle size, biomass loading, and IL/buffer ratio. DSD offered several solutions for optimization in which they were experimentally tested. Results The maximum sugar concentration (77 g/L) was obtained at 90 C, 70–116 min of treatment, 36–42 h of hydrolysis, 40–62 Unit/g cellulase loading, 34–35 %, w/v) biomass loading, 220–450 l particle size, and 10 % (v/v) IL to buffer ratio, respectively. The subsequent optimization by response surface methodology (RSM) revealed the temperature for treatment can drop to 75 C while fixing the pretreatment time at 100 min. Around 75 unit/g of cellulase and [22.0 % (w/v) of the biomass could be loaded to achieve a minimum of 70.0 ± 7.83 g/L of sugar, equivalent to 0.38 ± 0.08 g glucose/g and 0.48 ± 0.05 g total reducing sugar/g dry EFB. Conclusions The locally produced cellulase (PKC-Cel) from Trichoderma reesei exhibited promising results in the single-step process and can be used as an efficient approach to be optimized for fermentation to bioethanol production. Springer 2017-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56454/1/Implementation%20of%20Definite%20Screening%20Design%20in%20Optimization%20of%20in%20Situ.pdf application/pdf en http://irep.iium.edu.my/56454/8/56454-Implementation%20of%20definite%20screening%20design%20in%20optimization%20of%20in%20situ%20hydrolysis%20of%20EFB%20in%20cholinium%20acetate%20and%20locally%20produced%20cellulase%20combined%20system_SCOPUS.pdf Elgharbawy, Amal A. and Alam, Md Zahangir and Kabbashi, Nassereldeen Ahmed and Moniruzzaman, Muhammad and Jamal, Parveen (2017) Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system. Waste and Biomass Valorization, 8 (3). pp. 838-850. ISSN 1877-2641 E-ISSN 1877-265X https://link.springer.com/article/10.1007/s12649-016-9638-6 10.1007/s12649-016-9638-6 |
| spellingShingle | TP155 Chemical engineering Elgharbawy, Amal A. Alam, Md Zahangir Kabbashi, Nassereldeen Ahmed Moniruzzaman, Muhammad Jamal, Parveen Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system |
| title | Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system |
| title_full | Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system |
| title_fullStr | Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system |
| title_full_unstemmed | Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system |
| title_short | Implementation of definite screening design in optimization of in situ hydrolysis of EFB in cholinium acetate and locally produced cellulase combined system |
| title_sort | implementation of definite screening design in optimization of in situ hydrolysis of efb in cholinium acetate and locally produced cellulase combined system |
| topic | TP155 Chemical engineering |
| url | http://irep.iium.edu.my/56454/ http://irep.iium.edu.my/56454/ http://irep.iium.edu.my/56454/ http://irep.iium.edu.my/56454/1/Implementation%20of%20Definite%20Screening%20Design%20in%20Optimization%20of%20in%20Situ.pdf http://irep.iium.edu.my/56454/8/56454-Implementation%20of%20definite%20screening%20design%20in%20optimization%20of%20in%20situ%20hydrolysis%20of%20EFB%20in%20cholinium%20acetate%20and%20locally%20produced%20cellulase%20combined%20system_SCOPUS.pdf |