The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants
Erwinia amylovora is a Gram-negative enterobacterium that causes a devastating blight disease on rosaceous plants. In this study, the structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of E. amylovora, was investigated in bacterial growth and pathogenesis in host...
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Pakistan Agricultural Scientists Forum
2017
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| Online Access: | http://irep.iium.edu.my/56080/ http://irep.iium.edu.my/56080/1/Yilmaz_JAPS_pak.pdf http://irep.iium.edu.my/56080/2/56080-The%20role%20of%20DSPF%2C%20the%20type%20III%20secretion%20chaperone%20of%20DspA_SCOPUS.pdf |
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| author | Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin |
| author_facet | Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin |
| author_sort | Aksoy, Hasan Murad |
| building | IIUM Repository |
| collection | Online Access |
| description | Erwinia amylovora is a Gram-negative enterobacterium that causes a devastating blight disease on rosaceous plants. In this study, the structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of E. amylovora, was investigated in bacterial growth and pathogenesis in host and non-host plants, including apple, strawberry, rose, Arabidopsis and tobacco. DspF genes were mutated through homologous recombination and the result was the arrest of disease development on apple, strawberry and rose, and of the hypersensitivity response in tobacco. In addition, bacterial development of the wild type was compared with that of dspF mutants in leaves of A. thaliana Columbia-5 (Col-5), and the resistant A. thaliana Landsberg erecta (Ler-0). It was concluded that DspF is essential for the bacterium to induce hypersensitive responce (HR) disease on host plants (Rosaceous family plant) and non-host plants (Arabidopsis thaliana). |
| first_indexed | 2025-11-14T16:41:04Z |
| format | Article |
| id | iium-56080 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:41:04Z |
| publishDate | 2017 |
| publisher | Pakistan Agricultural Scientists Forum |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-560802017-07-18T06:29:22Z http://irep.iium.edu.my/56080/ The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin Q Science (General) Erwinia amylovora is a Gram-negative enterobacterium that causes a devastating blight disease on rosaceous plants. In this study, the structure of DspF, a type III secretion system (T3SS) chaperone required for virulence of E. amylovora, was investigated in bacterial growth and pathogenesis in host and non-host plants, including apple, strawberry, rose, Arabidopsis and tobacco. DspF genes were mutated through homologous recombination and the result was the arrest of disease development on apple, strawberry and rose, and of the hypersensitivity response in tobacco. In addition, bacterial development of the wild type was compared with that of dspF mutants in leaves of A. thaliana Columbia-5 (Col-5), and the resistant A. thaliana Landsberg erecta (Ler-0). It was concluded that DspF is essential for the bacterium to induce hypersensitive responce (HR) disease on host plants (Rosaceous family plant) and non-host plants (Arabidopsis thaliana). Pakistan Agricultural Scientists Forum 2017-02 Article PeerReviewed application/pdf en http://irep.iium.edu.my/56080/1/Yilmaz_JAPS_pak.pdf application/pdf en http://irep.iium.edu.my/56080/2/56080-The%20role%20of%20DSPF%2C%20the%20type%20III%20secretion%20chaperone%20of%20DspA_SCOPUS.pdf Aksoy, Hasan Murad and Kaya, Yilmaz and Tengku Abdul Hamid, Tengku Haziyamin (2017) The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants. The Journal of Animal & Plant Sciences, 27 (1). pp. 309-315. ISSN 1018-7081 http://www.thejaps.org.pk/docs/v-27-1/38.pdf |
| spellingShingle | Q Science (General) Aksoy, Hasan Murad Kaya, Yilmaz Tengku Abdul Hamid, Tengku Haziyamin The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants |
| title | The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants |
| title_full | The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants |
| title_fullStr | The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants |
| title_full_unstemmed | The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants |
| title_short | The role of DSPF, the type III secretion chaperone of DspA/E of Erwinia amylovora, in pathogenicity in host and non-host plants |
| title_sort | role of dspf, the type iii secretion chaperone of dspa/e of erwinia amylovora, in pathogenicity in host and non-host plants |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/56080/ http://irep.iium.edu.my/56080/ http://irep.iium.edu.my/56080/1/Yilmaz_JAPS_pak.pdf http://irep.iium.edu.my/56080/2/56080-The%20role%20of%20DSPF%2C%20the%20type%20III%20secretion%20chaperone%20of%20DspA_SCOPUS.pdf |