Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar

One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the application of cellulases produced from fermentation broth of sewage sludge on lignocel...

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Main Authors: Alam, Md. Zahangir, Mukhtaruddin, Muna Tasnim, Hamzah, Mohd. Salleh
Format: Proceeding Paper
Language:English
Published: 2010
Subjects:
Online Access:http://irep.iium.edu.my/5584/
http://irep.iium.edu.my/5584/1/Full-paper-ICER-2010.pdf
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author Alam, Md. Zahangir
Mukhtaruddin, Muna Tasnim
Hamzah, Mohd. Salleh
author_facet Alam, Md. Zahangir
Mukhtaruddin, Muna Tasnim
Hamzah, Mohd. Salleh
author_sort Alam, Md. Zahangir
building IIUM Repository
collection Online Access
description One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the application of cellulases produced from fermentation broth of sewage sludge on lignocellulosic materials such as rice straw and waste paper. In addition, the enzyme also has been applied on sewage sludge. The amount of sugar produced from the selected biomasses indicated whether the enzyme produced is in fact suitable to be used on large scale for the production of bioethanol. For the application on waste paper and rice straw, process parameters such as particles sizes and enzyme dosage were optimized in order to achieve high yield of sugar produced. While for the sewage sludge, the enzyme dosage and sludge concentration (percentage of Total Suspended Solid, TSS) were varied to observe the amount of reducing sugar. Using the cellulase enzyme with the activity of 45 U/ml, the waste paper showed the strongest susceptibility towards enzymatic hydrolysis reacted with cellulase enzyme. The amount of reducing sugar from the optimized condition for both waste paper and rice straw were 34.4 mg/ml and 18 mg/ml respectively which gave 68 and 36% of percentage of saccharification respectively. Whereas, for the application on the sewage sludge, after varying the sludge concentration and enzyme dosage, no reducing sugar was obtained after the saccharification process.
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spelling iium-55842021-06-23T03:55:23Z http://irep.iium.edu.my/5584/ Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar Alam, Md. Zahangir Mukhtaruddin, Muna Tasnim Hamzah, Mohd. Salleh TP Chemical technology TP248.13 Biotechnology One of the major applications of cellulase lies in the process of hydrolysis of cellulosic or lignocellulosic material to produce sugar which can be further fermented to produce ethanol. This study focuses on the application of cellulases produced from fermentation broth of sewage sludge on lignocellulosic materials such as rice straw and waste paper. In addition, the enzyme also has been applied on sewage sludge. The amount of sugar produced from the selected biomasses indicated whether the enzyme produced is in fact suitable to be used on large scale for the production of bioethanol. For the application on waste paper and rice straw, process parameters such as particles sizes and enzyme dosage were optimized in order to achieve high yield of sugar produced. While for the sewage sludge, the enzyme dosage and sludge concentration (percentage of Total Suspended Solid, TSS) were varied to observe the amount of reducing sugar. Using the cellulase enzyme with the activity of 45 U/ml, the waste paper showed the strongest susceptibility towards enzymatic hydrolysis reacted with cellulase enzyme. The amount of reducing sugar from the optimized condition for both waste paper and rice straw were 34.4 mg/ml and 18 mg/ml respectively which gave 68 and 36% of percentage of saccharification respectively. Whereas, for the application on the sewage sludge, after varying the sludge concentration and enzyme dosage, no reducing sugar was obtained after the saccharification process. 2010-08-16 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/5584/1/Full-paper-ICER-2010.pdf Alam, Md. Zahangir and Mukhtaruddin, Muna Tasnim and Hamzah, Mohd. Salleh (2010) Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar. In: 3rd International Congress of Environmental Research (ICER-10), 16-18 September 2010, University of Mauritius, Reduit, Mauritius.
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Alam, Md. Zahangir
Mukhtaruddin, Muna Tasnim
Hamzah, Mohd. Salleh
Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar
title Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar
title_full Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar
title_fullStr Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar
title_full_unstemmed Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar
title_short Application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar
title_sort application of cellulase enzyme obtained from fermentation of sewage sludge on rice straw into sugar
topic TP Chemical technology
TP248.13 Biotechnology
url http://irep.iium.edu.my/5584/
http://irep.iium.edu.my/5584/1/Full-paper-ICER-2010.pdf