Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge
An optimization based on statistical design method was employed to optimize the media constituents for the cellulase production from sewage sludge through liquid state bioconversion (LSB) by filamentous fungus Trichoderma reesei RUT C-30. The statistical analysis of experimental result showed that t...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2009
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| Online Access: | http://irep.iium.edu.my/5576/ http://irep.iium.edu.my/5576/1/Paper_for_media_optimization-cellulase.pdf |
| _version_ | 1848776528602595328 |
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| author | Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu |
| author_facet | Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu |
| author_sort | Drani , Afifah |
| building | IIUM Repository |
| collection | Online Access |
| description | An optimization based on statistical design method was employed to optimize the media constituents for the cellulase production from sewage sludge through liquid state bioconversion (LSB) by filamentous fungus Trichoderma reesei RUT C-30. The statistical analysis of experimental result showed that the optimum media constituent for cellulase production using sewage sludge as the major substrate, containing 1.5% cellulose and 0.5% peptone, supplemented with 0.25% KH2PO4, 0.2% tween 80, and 0.03% MgSO4 gave the maximum cellulase activity of 21.33 U/ml (CMCase activity). Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.92 with very low probability value (p < 0.0001) for the developed model. The present model is a good prediction of the experimental results and it is highly significant. |
| first_indexed | 2025-11-14T14:31:32Z |
| format | Proceeding Paper |
| id | iium-5576 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:31:32Z |
| publishDate | 2009 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-55762011-11-22T00:26:16Z http://irep.iium.edu.my/5576/ Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu TP Chemical technology TP248.13 Biotechnology An optimization based on statistical design method was employed to optimize the media constituents for the cellulase production from sewage sludge through liquid state bioconversion (LSB) by filamentous fungus Trichoderma reesei RUT C-30. The statistical analysis of experimental result showed that the optimum media constituent for cellulase production using sewage sludge as the major substrate, containing 1.5% cellulose and 0.5% peptone, supplemented with 0.25% KH2PO4, 0.2% tween 80, and 0.03% MgSO4 gave the maximum cellulase activity of 21.33 U/ml (CMCase activity). Analysis of variance (ANOVA) showed a high coefficient of determination (R2) of 0.92 with very low probability value (p < 0.0001) for the developed model. The present model is a good prediction of the experimental results and it is highly significant. 2009-05-19 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/5576/1/Paper_for_media_optimization-cellulase.pdf Drani , Afifah and Alam, Md. Zahangir and Muyibi, Suleyman Aremu (2009) Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge. In: Water Malaysia 2009 Conference & Exhibition, 19-21 May 2009, Kuala Lumpur. (Unpublished) http://www.watermalaysia.com/index.php?option=com_content&view=article&id=13:welcome-to-watermalaysiacom&catid=13:example-category |
| spellingShingle | TP Chemical technology TP248.13 Biotechnology Drani , Afifah Alam, Md. Zahangir Muyibi, Suleyman Aremu Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge |
| title | Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge |
| title_full | Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge |
| title_fullStr | Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge |
| title_full_unstemmed | Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge |
| title_short | Sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge |
| title_sort | sewage sludge as potential resource for the production of cellulase: statistical optimization of media constituent of sludge |
| topic | TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5576/ http://irep.iium.edu.my/5576/ http://irep.iium.edu.my/5576/1/Paper_for_media_optimization-cellulase.pdf |