Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase
The increase of insecticide space spraying to control Aedes mosquitoes submits vector control operators to low-level exposure to toxic organophosphates. Plasma β-glucuronidase level is found to increase in most organophosphate poisoning cases. This study aims to identify presence of organophosphat...
| Main Authors: | , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
University Publication Centre (UPENA) UiTM
2011
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/55399/ http://irep.iium.edu.my/55399/3/11.%20Ashraf%20ProceedingsUiTM2011.pdf |
| _version_ | 1848784572431466496 |
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| author | Rostam, Muhamad Ashraf Hassan, Zuridah |
| author_facet | Rostam, Muhamad Ashraf Hassan, Zuridah |
| author_sort | Rostam, Muhamad Ashraf |
| building | IIUM Repository |
| collection | Online Access |
| description | The increase of insecticide space spraying to control Aedes mosquitoes submits vector control operators to
low-level exposure to toxic organophosphates. Plasma β-glucuronidase level is found to increase in most
organophosphate poisoning cases. This study aims to identify presence of organophosphate poisoning using
β-glucuronidase assay as an alternative biomarker. A total of 180 blood samples (74 controls and 106
subjects) were examined for this enzyme using a spectrophotometer. These results were then compared to
the routine screening assay, butyrylcholinesterase (BChE) assay using a chemical analyzer. Both results
showed absence of organophosphate poisoning indicated by no increased in β-glucuronidase level with no
reduced in BChE level. Results of BChE level in the subject group (7879.70 ±227.935 IU/L), was
significantly higher than the control group (7067.86 ±1609.712 IU/L) (p<0.05). Meanwhile, β-
glucuronidase level between the subject (194.48 ±114.711 U/dL) and control group (192.60 ±122.685
U/dL) showed no significant difference (p>0.05). The findings of this evaluation show that assaying for the
β-glucuronidase enzyme can be an alternative method to determine organophosphate poisoning.
Furthermore, β-glucuronidase assay using spectrophotometer is recommended due to its ease of use and
suitability for small laboratories with limited facilities. |
| first_indexed | 2025-11-14T16:39:23Z |
| format | Proceeding Paper |
| id | iium-55399 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:39:23Z |
| publishDate | 2011 |
| publisher | University Publication Centre (UPENA) UiTM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-553992017-04-20T01:59:05Z http://irep.iium.edu.my/55399/ Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase Rostam, Muhamad Ashraf Hassan, Zuridah R Medicine (General) The increase of insecticide space spraying to control Aedes mosquitoes submits vector control operators to low-level exposure to toxic organophosphates. Plasma β-glucuronidase level is found to increase in most organophosphate poisoning cases. This study aims to identify presence of organophosphate poisoning using β-glucuronidase assay as an alternative biomarker. A total of 180 blood samples (74 controls and 106 subjects) were examined for this enzyme using a spectrophotometer. These results were then compared to the routine screening assay, butyrylcholinesterase (BChE) assay using a chemical analyzer. Both results showed absence of organophosphate poisoning indicated by no increased in β-glucuronidase level with no reduced in BChE level. Results of BChE level in the subject group (7879.70 ±227.935 IU/L), was significantly higher than the control group (7067.86 ±1609.712 IU/L) (p<0.05). Meanwhile, β- glucuronidase level between the subject (194.48 ±114.711 U/dL) and control group (192.60 ±122.685 U/dL) showed no significant difference (p>0.05). The findings of this evaluation show that assaying for the β-glucuronidase enzyme can be an alternative method to determine organophosphate poisoning. Furthermore, β-glucuronidase assay using spectrophotometer is recommended due to its ease of use and suitability for small laboratories with limited facilities. University Publication Centre (UPENA) UiTM 2011 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/55399/3/11.%20Ashraf%20ProceedingsUiTM2011.pdf Rostam, Muhamad Ashraf and Hassan, Zuridah (2011) Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase. In: UiTM Sarawak Conference 2011, Kuching, Sarawak. |
| spellingShingle | R Medicine (General) Rostam, Muhamad Ashraf Hassan, Zuridah Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase |
| title | Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase |
| title_full | Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase |
| title_fullStr | Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase |
| title_full_unstemmed | Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase |
| title_short | Assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase |
| title_sort | assessment of insecticide poisoning among vector control operators using a new biomarker, the enyzme β-glucuronidase |
| topic | R Medicine (General) |
| url | http://irep.iium.edu.my/55399/ http://irep.iium.edu.my/55399/3/11.%20Ashraf%20ProceedingsUiTM2011.pdf |