Effects of SRY (Sex Determining Region Y)-Box 9 (SOX9) and Telomerase Reverse Transcriptase (TERT) genes transfection in chondrocytes seeded on three-dimensional scaffolds: gross observation and cell proliferation assay

QR Code

Effects of SRY (Sex Determining Region Y)-Box 9 (SOX9) and Telomerase Reverse Transcriptase (TERT) genes transfection in chondrocytes seeded on three-dimensional scaffolds: gross observation and cell proliferation assay

Purpose: To evaluate the formation of in vitro 3D tissue constructs engineered from the SOX9 and TERT genes post-transfected chondrocytes seeded on poly(lactic-co-glycolic acid)(PLGA) based scaffolds. Methods: With the approval of Institutional Animal Care and Use Committee (IACUC,IIUM), the post-t...

Full description

Bibliographic Details
Main Authors:	Md Nazir, Noorhidayah, Zulkifly, Ahmad Hafiz, Khalid, Kamarul Ariffin, Zainol, Ismail, Zamli, Zaitunnatakhin, Sha'ban, Munirah
Format:	Proceeding Paper
Language:	English English
Published:	6 Frontiers Media S.A 2016
Subjects:	TA164 Bioengineering
Online Access:	http://irep.iium.edu.my/55027/ http://irep.iium.edu.my/55027/1/Noorhidayah%206thMTERMS2016%20Abstract.pdf http://irep.iium.edu.my/55027/7/55027%206thMTERMS2016%20Published%20Abstract.pdf

Similar Items

In vitro and in vivo non-viral SRY (Sex Determining Region Y)-box 9 (SOX9) and telomerase reverse transcriptase (TERT) genes transfer in chondrocytes: work in progress
by: Md Nazir, Noorhidayah, et al.
Published: (2016)

Viability and glycosaminoglycan content in chondrocytes transfected with SRY (sex-determining region y)-BOX 9 and telomerase reverse transcriptase genes
by: Md Nazir, Noorhidayah, et al.
Published: (2016)

The cartilaginous tissue formation using sry (Sex Determining Region Y)-BOX9 and telomerase reverse transcriptase genes transfected chondrocytes: in vivo approach
by: Noorhidayah Md Nazir,, et al.
Published: (2020)

Matrix production in chondrocytes transfected with sex determining region Y-Box 9 and telomerase reverse transcriptase genes: an in vitro evaluation from monolayer culture to three-dimensional culture
by: Md Nazir, Noorhidayah, et al.
Published: (2019)

The cartilaginous tissue formation using sry (Sex determining region Y)-BOX9 and telomerase reverse transcriptase genes transfected chondrocytes: In vivo approach = Formasi tisu tulang rawan menggunakan sry (Penentu jantina rantau Y)-BOX9 dan telomeras gen transkripsi berbalik kondrosit transfeksi: Pendekatan in vivo)
by: Md Nazir, Noorhidayah, et al.
Published: (2020)

Identifying transfection efficiency and cartilaginous markers expression in chondrocytes overexpressed with SRY (Sex Determining Region Y)-Box 9 (SOX9) gene: A preliminary analysis in an in vitro model
by: Md Nazir, Noorhidayah, et al.
Published: (2016)

Sox-9 transient transfection enhances chondrogenic expression of osteoarthritic human articular chondrocytes in vitro: preliminary analysis
by: Sha'ban, Munirah, et al.
Published: (2011)

Chondrocytes-induced SOX5/6/9 and TERT genes for articular cartilage tissue engineering: hype or hope?
by: Md Ali @ Tahir, Aisyah Hanani, et al.
Published: (2017)

Cartilaginous markers expression in human articular chondrocytes overexpressed with SOX9 gene
by: Mohamad Sukri, Norhamiza, et al.
Published: (2014)

Regulation Of Telomerase Reverse Transcriptase (Tert) By The Leukaemic Fusion Gene Aml1/Eto
by: Moses, Emmanuel Jairaj
Published: (2015)

In vitro and in vivo gene expression studies of cartilage-like tissue engineered construct using a combination of transiently transfected human osteoarthritic chondrocytes and tissue engineering technique
by: Sha'ban, Munirah, et al.
Published: (2016)

Identifying the potential of transcription factor Sox9 gene transfer in chondrocytes differentiation and articular cartilage formation in vitro
by: Mohamad Sukri, Norhamiza, et al.
Published: (2015)

Deciphering S-Phase Protein Kinase 2 (Skp2) Mediated Regulation Of Telomerase Reverse Transcriptase (Tert) In Kasumi-1, T (8;21) Acute Myeloid Leukemia (Aml) Cell Line
by: Rosli, Nur Aliaa Arina
Published: (2023)

Examining the safety and efficacy of non-viral gene transfer in cartilage tissue engineering from the worldview of Islam
by: Md Nazir, Noorhidayah, et al.
Published: (2016)

The re-expression of Collagen Type II, Aggrecan and Sox 9 in Tissue-Engineered Human Articular Cartilage
by: Sha'ban, Munirah, et al.
Published: (2005)

Chondrocyte apoptosis: a cause or consequence of osteoarthritis?
by: Zamli, Zaitunnatakhin, et al.
Published: (2011)

SOX9 predicts progression towards cirrhosis in patients while its loss protects against liver fibrosis
by: Athwal, Varinder S., et al.
Published: (2017)

Influence of 1α, 25-dihydroxyvitamin D3 [1, 25(OH)2D3] on the expression of Sox 9 and the transient receptor potential vanilloid 5/6 ion channels in equine articular chondrocytes
by: Hdud, Ismail M., et al.
Published: (2014)

3-Dimensional Poly(Lactic-co-Glycolic Acid) (PLGA), Fibrin and Atelocollagen Bioscaffold for Intervertebral Disc (IVD) Tissue Engineering
by: Sha'ban, Munirah
Published: (2016)

Collagen I and collagen II immunohistochemistry analyses on in vitro 3D poly(lactic-co-glycolic acid) seeded with intervertebral disc cells with and without fibrin scaffold
by: Mohamed Amin, Muhammad Azri Ifwat, et al.
Published: (2016)

Articular cartilage tissue engineering using poly(lactic-co-glycolic acid) based scaffolds seeded with bone marrow mesenchymal stem cells: from in vitro to in vivo model
by: Abdul Rahman, Rozlin, et al.
Published: (2016)

Swelling capacity and degradation behaviour of poly(lactic-co-glycolic acid) tissue engineering scaffold through atelocollagen, fibrin or, combination of atelocollagen and fibrin addition
by: Mohamad, Mohd Yusof, et al.
Published: (2016)

Fabrication and evaluation of mechanical strength of poly(lactic-co-glycolic acid) scaffold incorporated with atelocollagen type II
by: Mohamad, Mohd Yusof, et al.
Published: (2016)

Histology staining on in vitro 3D poly(lactic-co-glycolic acid) seeded with annulus fibrosus, nucleus pulposus, and a combination of annulus fibrosus: nucleus pulposus (1:1) cells with and without fibrin scaffold
by: Mohd. Azharuddin, Nur Syamimi, et al.
Published: (2016)

Fabrication and characterization of three-dimensional poly(lactic acid-co-glycolic acid), atelocollagen, and fibrin bioscaffold composite for intervertebral disk tissue engineering application
by: Mohamad, Mohd Yusof, et al.
Published: (2017)

Investigation of boldine as a potential telomerase inhibitor by downregulation of hTERT/hTERC in HCT 116 human colon carcinoma cells
by: Nadhirah Ahmad,, et al.
Published: (2019)

Influence of tert gene expression, telomerase activity and telomere length in relation to imatinib mesylate resistance in Malaysian chronic myeloid leukaemia patients
by: Shamshudin, Wati @ Hayati Mohd
Published: (2020)

Engineered meniscus scaffolds using sonication decellularization treatment system
by: Yusof, Fatihah, et al.
Published: (2019)

Identifying the potential of Qur’anic recitation on the proliferation of chondrocytes derived from rabbit articular cartilage: work in progress
by: Hashim, Rosyafirah, et al.
Published: (2018)

Identifying the potential of qur’anic recitation on the proliferation of chondrocytes derived from rabbit articular cartilage: work in progress
by: Hashim, Rosyafirah, et al.
Published: (2016)

Measurement of sulphated glycosaminoglycans and deoxyribonucleic acid content in annulus fibrosus intervertebral disc cells seeded into 3-dimensional poly(Lactic-Co-Glycolic Acid) based scaffolds
by: Mohamed Amin, Muhammad Azri Ifwat, et al.
Published: (2016)

Tissue engineering and regenerative medicine research and development in Malaysia: A scientometric study based on relevant conferences from 2004 to 2014
by: Mohd. Azharuddin, Nur Syamimi, et al.
Published: (2016)

Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 transfection of guide RNA targeting on MMP9 as anti-cancer therapy in human cutaneous squamous cell carcinoma cell line A431
by: Teh, Seoh Wei, et al.
Published: (2023)

Human platelet lysate promotes proliferation but fails to maintain chondrogenic markers of chondrocytes
by: Prana Hardinata Budi Harto,, et al.
Published: (2019)

Early response in antibacterial activity of orthopaedic metal implant coated with silver composite for future osteomyelitis treatment: preliminary results
by: Mohd Jan, Nurul Hafiza, et al.
Published: (2016)

Poly(lactic-co-glycolic acid) and atelocollagen hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
by: Mohamad, Mohd Yusof, et al.
Published: (2016)

Poly(lactic-co-glycolic acid), atelocollagen and fibrin hybrid scaffold seeded with annulus fibrosus cells enhances the formation of cartilaginous tissue engineered construct in vitro
by: Mohamed Amin, Muhammad Azri Ifwat, et al.
Published: (2016)

In vivo evaluation of 3-dimensional PLGA/ Atelocollagen/ Fibrin scaffolds for intervertebral disc (IVD) regeneration
by: Mohamad, Mohd Yusof, et al.
Published: (2016)

Monolayer culture expansion of Annulus fibrosus cells for intervertebral disc regeneration
by: Mohamad, Mohd Yusof, et al.
Published: (2016)

Physical properties and biocompatibility of 3D hybrid PLGA based scaffolds
by: Mohamed Amin, Muhammad Azri Ifwat, et al.
Published: (2019)