Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro

This research aims to extract and characterize polysaccharides from defatted coconut residue (DCR) and determine their potential use as prebiotic. The extraction of the polysaccharides has yielded about 0.73 ± 0.04%. The polysaccharides have been characterised the monosaccharide composition using HP...

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Main Author: Marikkar, Mohammed Nazrim
Format: Article
Language:English
Published: Springer 2016
Subjects:
Online Access:http://irep.iium.edu.my/54460/
http://irep.iium.edu.my/54460/1/PAPER-Online%20version.pdf
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author Marikkar, Mohammed Nazrim
author_facet Marikkar, Mohammed Nazrim
author_sort Marikkar, Mohammed Nazrim
building IIUM Repository
collection Online Access
description This research aims to extract and characterize polysaccharides from defatted coconut residue (DCR) and determine their potential use as prebiotic. The extraction of the polysaccharides has yielded about 0.73 ± 0.04%. The polysaccharides have been characterised the monosaccharide composition using HPLC-RI and β-glycosidic bond assessment using FTIR. The presence of β-glycosidic bond in DCR has been confirmed by the presence of peak around 893 cm-1. Result showed that the polysaccharides contain about 6.95% of total carbohydrate and 20.71% of reducing sugar. Prebiotic potential of the polysaccharides was determined by using the polysaccharides as carbon source in the basal medium. The plate count results showed, there was a significant increase in the Lactobacillus casei Shirota strain and it was comparable to commercial Inulin. They also suppressed the growth of E.coli ATCC 1129. It can be concluded that polysaccharides from coconut waste can be a good alternatives for prebiotic sources in the future.
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spelling iium-544602017-01-18T04:24:47Z http://irep.iium.edu.my/54460/ Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro Marikkar, Mohammed Nazrim QD Chemistry This research aims to extract and characterize polysaccharides from defatted coconut residue (DCR) and determine their potential use as prebiotic. The extraction of the polysaccharides has yielded about 0.73 ± 0.04%. The polysaccharides have been characterised the monosaccharide composition using HPLC-RI and β-glycosidic bond assessment using FTIR. The presence of β-glycosidic bond in DCR has been confirmed by the presence of peak around 893 cm-1. Result showed that the polysaccharides contain about 6.95% of total carbohydrate and 20.71% of reducing sugar. Prebiotic potential of the polysaccharides was determined by using the polysaccharides as carbon source in the basal medium. The plate count results showed, there was a significant increase in the Lactobacillus casei Shirota strain and it was comparable to commercial Inulin. They also suppressed the growth of E.coli ATCC 1129. It can be concluded that polysaccharides from coconut waste can be a good alternatives for prebiotic sources in the future. Springer 2016-12-15 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54460/1/PAPER-Online%20version.pdf Marikkar, Mohammed Nazrim (2016) Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro. Journal of Food Science and Technology-Mysor, Not available (Not available). ISSN 0022-1155 E-ISSN 0975-8402 (In Press) http://www.springer.com/food+science/journal/13197 10.1007/s13197-016-2448-9
spellingShingle QD Chemistry
Marikkar, Mohammed Nazrim
Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
title Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
title_full Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
title_fullStr Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
title_full_unstemmed Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
title_short Defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
title_sort defatted coconut residue polysaccharides as potential prebiotics: study of their effects on proliferation and acidifying activity of probiotics in vitro
topic QD Chemistry
url http://irep.iium.edu.my/54460/
http://irep.iium.edu.my/54460/
http://irep.iium.edu.my/54460/
http://irep.iium.edu.my/54460/1/PAPER-Online%20version.pdf