Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma
Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent k...
| Main Authors: | , , , , , |
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| Format: | Proceeding Paper |
| Language: | English English |
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IIUM
2016
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| Online Access: | http://irep.iium.edu.my/54378/ http://irep.iium.edu.my/54378/1/MRS2016-ABSTRACT-BOOK-5.pdf http://irep.iium.edu.my/54378/7/54378_epigenetic%20methylation.pdf |
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| author | Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza |
| author_facet | Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza |
| author_sort | Mohd. Ridah, Lailatul Jalilah |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent kinase, which results in retinoblastoma phosphorylation and blockage of cell cycle at G1 phase. MGMT removes alkyl adduct at O6-guanine, thus preventing lethal cross-links. SPOCK2, an extracellular chondroitin and heparin sulfate proteoglycans, abolishes the inhibition of membrane-type 1-matrix metalloproteinase which might enhance the angiogenesis. The absence of SPOCK2 methylation was therefore hypothesized in the majority of cases in this study. Methods: Extracted DNA from 88 formalin-fixed paraffin-embedded (FFPE) tissues of DLBCL were subjected to bisulfite conversion followed by methylation-specific PCR (MSP) analysis for p16, MGMT and SPOCK2 methylation. p16 methylation was also quantified in 16 samples through pyrosequencing assay. Results: p16 methylation was observed in 65/88 (74%) samples by MSP. Pyrosequencing detected p16 methylation in all 16 samples ranging from 18% to 81%. MGMT methylation was detected in all 88 (100%) cases. Methylated SPOCK2 was found in 83 (94.3%) samples. There was a significant association between p16 methylation status with patients above 50 years of age (p= 0.04). Conclusions: These preliminary discoveries may serve as a good platform in order to gain a comprehensive overview on the epigenetics contribution in the pathogenesis of DLBCL. Pyrosequencing is a robust tool in detecting and quantifying methylation. |
| first_indexed | 2025-11-14T16:36:37Z |
| format | Proceeding Paper |
| id | iium-54378 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:36:37Z |
| publishDate | 2016 |
| publisher | IIUM |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-543782022-04-07T01:55:46Z http://irep.iium.edu.my/54378/ Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza RC254 Neoplasms. Tumors. Oncology (including Cancer) Introduction: Epigenetic methylation has been implicated in the pathogenesis of diffuse large B cell lymphoma (DLBCL). This study investigated the methylation status of p16, MGMT and SPOCK2. Aberrantly methylated p16 and MGMT have been linked to DLBCL, but not SPOCK2. p16 inhibits cyclin-dependent kinase, which results in retinoblastoma phosphorylation and blockage of cell cycle at G1 phase. MGMT removes alkyl adduct at O6-guanine, thus preventing lethal cross-links. SPOCK2, an extracellular chondroitin and heparin sulfate proteoglycans, abolishes the inhibition of membrane-type 1-matrix metalloproteinase which might enhance the angiogenesis. The absence of SPOCK2 methylation was therefore hypothesized in the majority of cases in this study. Methods: Extracted DNA from 88 formalin-fixed paraffin-embedded (FFPE) tissues of DLBCL were subjected to bisulfite conversion followed by methylation-specific PCR (MSP) analysis for p16, MGMT and SPOCK2 methylation. p16 methylation was also quantified in 16 samples through pyrosequencing assay. Results: p16 methylation was observed in 65/88 (74%) samples by MSP. Pyrosequencing detected p16 methylation in all 16 samples ranging from 18% to 81%. MGMT methylation was detected in all 88 (100%) cases. Methylated SPOCK2 was found in 83 (94.3%) samples. There was a significant association between p16 methylation status with patients above 50 years of age (p= 0.04). Conclusions: These preliminary discoveries may serve as a good platform in order to gain a comprehensive overview on the epigenetics contribution in the pathogenesis of DLBCL. Pyrosequencing is a robust tool in detecting and quantifying methylation. IIUM 2016-12 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/54378/1/MRS2016-ABSTRACT-BOOK-5.pdf application/pdf en http://irep.iium.edu.my/54378/7/54378_epigenetic%20methylation.pdf Mohd. Ridah, Lailatul Jalilah and Ismail, Aziah and A. Talib, Norlelawati and Muhammad, Siti Aeshah @ Naznin and Hussain, Faezahtul Arbaiyah and Zainuddin, Norafiza (2016) Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang. http://iiumedic.net/imjm/v1/ |
| spellingShingle | RC254 Neoplasms. Tumors. Oncology (including Cancer) Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma |
| title | Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma |
| title_full | Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma |
| title_fullStr | Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma |
| title_full_unstemmed | Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma |
| title_short | Epigenetic methylation status of P16, MGMT and SPOCK2 in diffuse Large B cell lymphoma |
| title_sort | epigenetic methylation status of p16, mgmt and spock2 in diffuse large b cell lymphoma |
| topic | RC254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://irep.iium.edu.my/54378/ http://irep.iium.edu.my/54378/ http://irep.iium.edu.my/54378/1/MRS2016-ABSTRACT-BOOK-5.pdf http://irep.iium.edu.my/54378/7/54378_epigenetic%20methylation.pdf |