Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia
Introduction: Methylation of promoter region of p16 leading to gene silencing has been implicated in a wide range of malignancies including lymphomas. In diffuse large B cell lymphoma (DLBCL) particularly, a varying percentage of epigenetic inactivation of p16 promoter region was observed ranging f...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universiti Sains Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/54196/ http://irep.iium.edu.my/54196/1/NZ_JCBS_61-58-PB.pdf |
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| author | Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza |
| author_facet | Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza |
| author_sort | Mohd. Ridah, Lailatul Jalilah |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Methylation of promoter region of p16 leading to gene silencing has been implicated in a wide range of malignancies including lymphomas. In diffuse large B cell lymphoma (DLBCL) particularly, a varying
percentage of epigenetic inactivation of p16 promoter region was observed ranging from 16 - 54%. However, quantitative analysis of p16 promoter methylation in DLBCL has not been extensively studied in Malaysia. Objective: This study aims to quantitatively analyse p16 methylation in DLBCL samples using pyrosequencing technique. Methods: Genomic DNA was extracted from 16 formalin-fixed paraffin-embedded lymphoma tissue blocks from patients diagnosed with DLBCL. Samples were retrieved from Hospital Tengku Ampuan Afzan, Pahang and Hospital Universiti Sains Malaysia, Kelantan. Primers were designed to amplify bisulfite-treated DNA targeting p16 promoter region. Methylation status of 7 CpG sites was determined by pyrosequencing. Results: All the 16 samples studied showed promoter methylation of p16. The
range of mean methylation percentage was between 18 to 81%. Conclusion: The present study has successfully measured the level of methylation of p16 in all 7 CpG sites despite the limitation in sample size. Since p16 methylation is a common event in our series of DLBCL cases, it is worth including a larger sample size in future studies to increase the chance of finding a significant correlation with clinical parameters. |
| first_indexed | 2025-11-14T16:36:06Z |
| format | Article |
| id | iium-54196 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:36:06Z |
| publishDate | 2016 |
| publisher | Universiti Sains Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-541962024-06-19T07:19:52Z http://irep.iium.edu.my/54196/ Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza RC254 Neoplasms. Tumors. Oncology (including Cancer) Introduction: Methylation of promoter region of p16 leading to gene silencing has been implicated in a wide range of malignancies including lymphomas. In diffuse large B cell lymphoma (DLBCL) particularly, a varying percentage of epigenetic inactivation of p16 promoter region was observed ranging from 16 - 54%. However, quantitative analysis of p16 promoter methylation in DLBCL has not been extensively studied in Malaysia. Objective: This study aims to quantitatively analyse p16 methylation in DLBCL samples using pyrosequencing technique. Methods: Genomic DNA was extracted from 16 formalin-fixed paraffin-embedded lymphoma tissue blocks from patients diagnosed with DLBCL. Samples were retrieved from Hospital Tengku Ampuan Afzan, Pahang and Hospital Universiti Sains Malaysia, Kelantan. Primers were designed to amplify bisulfite-treated DNA targeting p16 promoter region. Methylation status of 7 CpG sites was determined by pyrosequencing. Results: All the 16 samples studied showed promoter methylation of p16. The range of mean methylation percentage was between 18 to 81%. Conclusion: The present study has successfully measured the level of methylation of p16 in all 7 CpG sites despite the limitation in sample size. Since p16 methylation is a common event in our series of DLBCL cases, it is worth including a larger sample size in future studies to increase the chance of finding a significant correlation with clinical parameters. Universiti Sains Malaysia 2016 Article PeerReviewed application/pdf en http://irep.iium.edu.my/54196/1/NZ_JCBS_61-58-PB.pdf Mohd. Ridah, Lailatul Jalilah and Ismail, Aziah and A. Talib, Norlelawati and Muhammad, Siti Aeshah @ Naznin and Hussain, Faezahtul Arbaiyah and Zainuddin, Norafiza (2016) Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia. Journal of Biomedical and Clinical Sciences, 1 (1). pp. 12-17. E-ISSN 2550-147X http://apps.amdi.usm.my/journal/index.php/jbcs/index |
| spellingShingle | RC254 Neoplasms. Tumors. Oncology (including Cancer) Mohd. Ridah, Lailatul Jalilah Ismail, Aziah A. Talib, Norlelawati Muhammad, Siti Aeshah @ Naznin Hussain, Faezahtul Arbaiyah Zainuddin, Norafiza Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia |
| title | Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia |
| title_full | Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia |
| title_fullStr | Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia |
| title_full_unstemmed | Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia |
| title_short | Pyrosequencing-based quantitative identification of p16 methylation in diffuse large B-cell lymphoma at two centres in the East Coast of Malaysia |
| title_sort | pyrosequencing-based quantitative identification of p16 methylation in diffuse large b-cell lymphoma at two centres in the east coast of malaysia |
| topic | RC254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://irep.iium.edu.my/54196/ http://irep.iium.edu.my/54196/ http://irep.iium.edu.my/54196/1/NZ_JCBS_61-58-PB.pdf |