Production of lycopene from tomato waste using solid state fermentation

Lycopene is a well-known carotenoid, causing the red color of fresh tomatoes. The significance of lycopene as antioxidant agent and coloring in the cosmetics, and its use in pharmaceutical and food industries has expanded in the recent years. Extraction of lycopene was improved effectively under sol...

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Main Authors: Jamal, Parveen, Haslamona, Ali, Jaswir, Irwandi, Akbar, Iqrah
Format: Proceeding Paper
Language:English
Published: Kulliyah of Engineering, International Islamic University Malaysia 2016
Subjects:
Online Access:http://irep.iium.edu.my/53989/
http://irep.iium.edu.my/53989/6/53989.pdf
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author Jamal, Parveen
Haslamona, Ali
Jaswir, Irwandi
Akbar, Iqrah
author_facet Jamal, Parveen
Haslamona, Ali
Jaswir, Irwandi
Akbar, Iqrah
author_sort Jamal, Parveen
building IIUM Repository
collection Online Access
description Lycopene is a well-known carotenoid, causing the red color of fresh tomatoes. The significance of lycopene as antioxidant agent and coloring in the cosmetics, and its use in pharmaceutical and food industries has expanded in the recent years. Extraction of lycopene was improved effectively under solid state fermentation process; whereby, cellulase produced from the fermentation process was employed to degrade the cell-wall constituents, which facilitated the release of intracellular contents. The optimum conditions for the fermentation process were determined using response surface methodology (RSM). A face-centered central composite design (FCCCD) was employed to investigate the effects of three independent factors: moisture content, inoculums size and incubation time. Twenty experiments were conducted and each one was replicated (repeated) three times. The obtained data was analyzed using design expert software version 6. Regression analysis showed that 94.56% of the variation was explained by the software. Under optimized conditions the highest lycopene yield was 307.2µg/g when the moisture content was 80%, the inoculums size was 15% in 4 incubation days. The experimental values agreed with the predicted values, thus proving stability of the model used and the success of RSM. This study showed as to how fermentation can improve the extraction process by comparing the result with the control (extraction without fermentation) which was 0.8µg/g.
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institution International Islamic University Malaysia
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language English
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spelling iium-539892017-01-17T00:48:27Z http://irep.iium.edu.my/53989/ Production of lycopene from tomato waste using solid state fermentation Jamal, Parveen Haslamona, Ali Jaswir, Irwandi Akbar, Iqrah TP Chemical technology TP248.13 Biotechnology Lycopene is a well-known carotenoid, causing the red color of fresh tomatoes. The significance of lycopene as antioxidant agent and coloring in the cosmetics, and its use in pharmaceutical and food industries has expanded in the recent years. Extraction of lycopene was improved effectively under solid state fermentation process; whereby, cellulase produced from the fermentation process was employed to degrade the cell-wall constituents, which facilitated the release of intracellular contents. The optimum conditions for the fermentation process were determined using response surface methodology (RSM). A face-centered central composite design (FCCCD) was employed to investigate the effects of three independent factors: moisture content, inoculums size and incubation time. Twenty experiments were conducted and each one was replicated (repeated) three times. The obtained data was analyzed using design expert software version 6. Regression analysis showed that 94.56% of the variation was explained by the software. Under optimized conditions the highest lycopene yield was 307.2µg/g when the moisture content was 80%, the inoculums size was 15% in 4 incubation days. The experimental values agreed with the predicted values, thus proving stability of the model used and the success of RSM. This study showed as to how fermentation can improve the extraction process by comparing the result with the control (extraction without fermentation) which was 0.8µg/g. Kulliyah of Engineering, International Islamic University Malaysia 2016-07 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/53989/6/53989.pdf Jamal, Parveen and Haslamona, Ali and Jaswir, Irwandi and Akbar, Iqrah (2016) Production of lycopene from tomato waste using solid state fermentation. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/
spellingShingle TP Chemical technology
TP248.13 Biotechnology
Jamal, Parveen
Haslamona, Ali
Jaswir, Irwandi
Akbar, Iqrah
Production of lycopene from tomato waste using solid state fermentation
title Production of lycopene from tomato waste using solid state fermentation
title_full Production of lycopene from tomato waste using solid state fermentation
title_fullStr Production of lycopene from tomato waste using solid state fermentation
title_full_unstemmed Production of lycopene from tomato waste using solid state fermentation
title_short Production of lycopene from tomato waste using solid state fermentation
title_sort production of lycopene from tomato waste using solid state fermentation
topic TP Chemical technology
TP248.13 Biotechnology
url http://irep.iium.edu.my/53989/
http://irep.iium.edu.my/53989/
http://irep.iium.edu.my/53989/6/53989.pdf