Effects of ionizing radiation on chondrocytes growth: work in progress
Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes. Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three...
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| Format: | Proceeding Paper |
| Language: | English English |
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2016
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| Online Access: | http://irep.iium.edu.my/53947/ http://irep.iium.edu.my/53947/1/NurFarhana.MTERMS2016.pdf http://irep.iium.edu.my/53947/2/NurFarhana.MTERMS2016_Oral.pdf |
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| author | Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim |
| author_facet | Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim |
| author_sort | Mat Nawi, Nur Farhana |
| building | IIUM Repository |
| collection | Online Access |
| description | Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes.
Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three groups; (1) control, (2) irradiated and (3) non-irradiated. Group 2 cells were subjected to irradiation after it reaches
80-90% confluency. While the control group was left in the incubator, groups 2 and 3 were taken out and put into a temperature-controlled container. They were transported to a
typical X-ray examination room, some distance away from the incubator. After irradiating Group 2, both cells groups were returned into the incubator. The X-ray imaging parameters and the collimation used were recorded. Group 2 cells were subjected to irradiation at each different passage, from P0 till P3. Morphological observation and growth kinetics assessments were conducted on all groups after each passage.
Results: There are no differences in terms of cellular morphology in all groups throughout passages. The viability of all groups ranged from 94.1-98.6%. All groups showed reduction in growth rate after several passages. The non-irradiated group exhibited higher total cell yield than the other groups. The performance of growth kinetics of all groups can be appreciated as follows; non-irradiated group > irradiated > control.
Conclusion: Ionizing radiation may have certain effects on the growth of chondrocytes. |
| first_indexed | 2025-11-14T16:35:24Z |
| format | Proceeding Paper |
| id | iium-53947 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:35:24Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-539472016-12-26T04:38:28Z http://irep.iium.edu.my/53947/ Effects of ionizing radiation on chondrocytes growth: work in progress Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim Q Science (General) Purpose: To evaluate the possible effects of ionizing radiation on cellular morphology and growth kinetics of monolayer cultured chondrocytes. Methods: Following research approval (IIUM/IACUC Approval/2015/[5][24]), rabbit’s articular cartilage cells were isolated, cultured and divided into three groups; (1) control, (2) irradiated and (3) non-irradiated. Group 2 cells were subjected to irradiation after it reaches 80-90% confluency. While the control group was left in the incubator, groups 2 and 3 were taken out and put into a temperature-controlled container. They were transported to a typical X-ray examination room, some distance away from the incubator. After irradiating Group 2, both cells groups were returned into the incubator. The X-ray imaging parameters and the collimation used were recorded. Group 2 cells were subjected to irradiation at each different passage, from P0 till P3. Morphological observation and growth kinetics assessments were conducted on all groups after each passage. Results: There are no differences in terms of cellular morphology in all groups throughout passages. The viability of all groups ranged from 94.1-98.6%. All groups showed reduction in growth rate after several passages. The non-irradiated group exhibited higher total cell yield than the other groups. The performance of growth kinetics of all groups can be appreciated as follows; non-irradiated group > irradiated > control. Conclusion: Ionizing radiation may have certain effects on the growth of chondrocytes. 2016-11-17 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/53947/1/NurFarhana.MTERMS2016.pdf application/pdf en http://irep.iium.edu.my/53947/2/NurFarhana.MTERMS2016_Oral.pdf Mat Nawi, Nur Farhana and Sha'ban, Munirah and Zainuddin, Zainul Ibrahim (2016) Effects of ionizing radiation on chondrocytes growth: work in progress. In: 6th MTERMS 2016 in conjunction with 2nd Malaysian Stem Cells Meeting, 17 - 18 Nov 2016, Penang. (Unpublished) |
| spellingShingle | Q Science (General) Mat Nawi, Nur Farhana Sha'ban, Munirah Zainuddin, Zainul Ibrahim Effects of ionizing radiation on chondrocytes growth: work in progress |
| title | Effects of ionizing radiation on chondrocytes growth: work in progress |
| title_full | Effects of ionizing radiation on chondrocytes growth: work in progress |
| title_fullStr | Effects of ionizing radiation on chondrocytes growth: work in progress |
| title_full_unstemmed | Effects of ionizing radiation on chondrocytes growth: work in progress |
| title_short | Effects of ionizing radiation on chondrocytes growth: work in progress |
| title_sort | effects of ionizing radiation on chondrocytes growth: work in progress |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/53947/ http://irep.iium.edu.my/53947/1/NurFarhana.MTERMS2016.pdf http://irep.iium.edu.my/53947/2/NurFarhana.MTERMS2016_Oral.pdf |