Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy
Introduction: Nuclear magnetic resonance (NMR) spectroscopy is an analytical chemistry technique used for the chemical structural determination. This valuable tool is used to study nuclei, commonly hydrogen (1H) and carbon (13C). NMR provides information about the number of atoms of the studied nucl...
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| Format: | Proceeding Paper |
| Language: | English English English |
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2016
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| Online Access: | http://irep.iium.edu.my/52614/ http://irep.iium.edu.my/52614/1/NMR%20Poster_Hawa.pdf http://irep.iium.edu.my/52614/2/Abstract_PP_Hawa.pdf http://irep.iium.edu.my/52614/3/Cover%20page.pdf |
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| author | Ab. Hadi, Hazrina Azmar, Hawa Mas |
| author_facet | Ab. Hadi, Hazrina Azmar, Hawa Mas |
| author_sort | Ab. Hadi, Hazrina |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Nuclear magnetic resonance (NMR) spectroscopy is an analytical chemistry technique used for the chemical structural determination. This valuable tool is used to study nuclei, commonly hydrogen (1H) and carbon (13C). NMR provides information about the number of atoms of the studied nuclei that are magnetically separated as well as the nature of the environment of the nuclei. Aims: In this study, NMR is used to identify the isolated hydroxychavicol. The isolated hydroxychavicol was dissolved in deuterated methanol. Methods: The sample is analyzed for 1D NMR (1H and 13C) spectra using 500 MHz NMR (Bruker; Avance III Ultrashield Plus; Switzerland) located in Universiti Malaysia Pahang. The target directed isolation of hydroxychavicol was performed by comparing bioautographic profile of hydroxychavicol-rich fraction to that of hydroxychavicol standard. Hydroxychavicol is a known compound with molecular formula of C9H10O2 and molecular weight of 150.174 g/mol. Results and discussion: 1H and 13C NMR elucidated the structure of the isolated hydroxychavicol. The 1H NMR spectrum furnished 10 protons at chemical shifts of δH 3.21, 4.90, 4.98, 5.9, 6.49, 6.61, and 6.67 ppm. Based on the spectrum, a broad spectrum was displayed meaning there is a hydroxyl group in the structure. The 13C NMR spectrum displayed the nine carbons at chemical shifts δC 40.64, 115.33, 116.31, 116.76, 120.87, 132.99, 139.57, 144.46, and 146.17 ppm. Conclusion: The isolated hydroxychavicol was successfully being authenticated by NMR. |
| first_indexed | 2025-11-14T16:31:41Z |
| format | Proceeding Paper |
| id | iium-52614 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English English |
| last_indexed | 2025-11-14T16:31:41Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-526142017-03-10T17:37:24Z http://irep.iium.edu.my/52614/ Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy Ab. Hadi, Hazrina Azmar, Hawa Mas RS Pharmacy and materia medica Introduction: Nuclear magnetic resonance (NMR) spectroscopy is an analytical chemistry technique used for the chemical structural determination. This valuable tool is used to study nuclei, commonly hydrogen (1H) and carbon (13C). NMR provides information about the number of atoms of the studied nuclei that are magnetically separated as well as the nature of the environment of the nuclei. Aims: In this study, NMR is used to identify the isolated hydroxychavicol. The isolated hydroxychavicol was dissolved in deuterated methanol. Methods: The sample is analyzed for 1D NMR (1H and 13C) spectra using 500 MHz NMR (Bruker; Avance III Ultrashield Plus; Switzerland) located in Universiti Malaysia Pahang. The target directed isolation of hydroxychavicol was performed by comparing bioautographic profile of hydroxychavicol-rich fraction to that of hydroxychavicol standard. Hydroxychavicol is a known compound with molecular formula of C9H10O2 and molecular weight of 150.174 g/mol. Results and discussion: 1H and 13C NMR elucidated the structure of the isolated hydroxychavicol. The 1H NMR spectrum furnished 10 protons at chemical shifts of δH 3.21, 4.90, 4.98, 5.9, 6.49, 6.61, and 6.67 ppm. Based on the spectrum, a broad spectrum was displayed meaning there is a hydroxyl group in the structure. The 13C NMR spectrum displayed the nine carbons at chemical shifts δC 40.64, 115.33, 116.31, 116.76, 120.87, 132.99, 139.57, 144.46, and 146.17 ppm. Conclusion: The isolated hydroxychavicol was successfully being authenticated by NMR. 2016 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/52614/1/NMR%20Poster_Hawa.pdf application/pdf en http://irep.iium.edu.my/52614/2/Abstract_PP_Hawa.pdf application/pdf en http://irep.iium.edu.my/52614/3/Cover%20page.pdf Ab. Hadi, Hazrina and Azmar, Hawa Mas (2016) Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy. In: Pharma BioAsia Conference 2016, 28-29 Sep 2016, Kuala Lumpur. (Unpublished) |
| spellingShingle | RS Pharmacy and materia medica Ab. Hadi, Hazrina Azmar, Hawa Mas Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy |
| title | Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy |
| title_full | Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy |
| title_fullStr | Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy |
| title_full_unstemmed | Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy |
| title_short | Authentication of isolated hydroxychavicol from p. Betle linn. Leaves using nuclear magnetic resonance spectroscopy |
| title_sort | authentication of isolated hydroxychavicol from p. betle linn. leaves using nuclear magnetic resonance spectroscopy |
| topic | RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/52614/ http://irep.iium.edu.my/52614/1/NMR%20Poster_Hawa.pdf http://irep.iium.edu.my/52614/2/Abstract_PP_Hawa.pdf http://irep.iium.edu.my/52614/3/Cover%20page.pdf |