Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae
Euplotes is a ciliated protozoan that is highly diverse in size and species and has poten-tial to be used as live feed for marine feed larvae. This study was conducted to identify the suitable Euplotes candidate through molecular and morphological methods. Euplotes sp. was characterized by its morph...
| Main Authors: | , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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2016
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| Online Access: | http://irep.iium.edu.my/52404/ http://irep.iium.edu.my/52404/1/52404_MORPHOLOGICAL%20AND%2018S%20rRNA.pdf |
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| author | Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori |
| author_facet | Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori |
| author_sort | Waludin, Shaidatul Nadia |
| building | IIUM Repository |
| collection | Online Access |
| description | Euplotes is a ciliated protozoan that is highly diverse in size and species and has poten-tial to be used as live feed for marine feed larvae. This study was conducted to identify the suitable Euplotes candidate through molecular and morphological methods. Euplotes sp. was characterized by its morphological and 18S rRNA gene sequence. The selected Euplotes sp. was identified morphologically based on its unique present of infracillia-ture, dorsal-ventrally flattened, dorsally ridged and oval in shaped. For molecular identi-fication, samples of Euplotes sp. were collected and starved overnight before pelleted through centrifugation to provide closely packed Euplotes species. The extraction and amplification of genomic DNA of Euplotes sp. were done using REDExtract-N-AmpTM Tissue PCR Kit (Sigma). The phylogenetic analyses of the SSU RNA gene was ana-lyzed with evolutionary distance calculated using Kimura-two-parameter and further analyzed by using Maximum Likelihood (ML) and Neighbor-joining (NJ) with Coris julis (accession number HM049942.1) as an out group. The result showed Euplotes sp. is 96% related to Euplotes encysticus (accession number LN864512.1) and Euplotes no-vemcarinatus (accession number HM140407.1). |
| first_indexed | 2025-11-14T16:31:06Z |
| format | Proceeding Paper |
| id | iium-52404 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:31:06Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-524042016-10-27T02:29:23Z http://irep.iium.edu.my/52404/ Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori SH Aquaculture. Fisheries. Angling Euplotes is a ciliated protozoan that is highly diverse in size and species and has poten-tial to be used as live feed for marine feed larvae. This study was conducted to identify the suitable Euplotes candidate through molecular and morphological methods. Euplotes sp. was characterized by its morphological and 18S rRNA gene sequence. The selected Euplotes sp. was identified morphologically based on its unique present of infracillia-ture, dorsal-ventrally flattened, dorsally ridged and oval in shaped. For molecular identi-fication, samples of Euplotes sp. were collected and starved overnight before pelleted through centrifugation to provide closely packed Euplotes species. The extraction and amplification of genomic DNA of Euplotes sp. were done using REDExtract-N-AmpTM Tissue PCR Kit (Sigma). The phylogenetic analyses of the SSU RNA gene was ana-lyzed with evolutionary distance calculated using Kimura-two-parameter and further analyzed by using Maximum Likelihood (ML) and Neighbor-joining (NJ) with Coris julis (accession number HM049942.1) as an out group. The result showed Euplotes sp. is 96% related to Euplotes encysticus (accession number LN864512.1) and Euplotes no-vemcarinatus (accession number HM140407.1). 2016-08 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/52404/1/52404_MORPHOLOGICAL%20AND%2018S%20rRNA.pdf Waludin, Shaidatul Nadia and Afand, Muhammad Mu’izzuddin and Mohammad Noor, Normawaty and Phang, Ing Chia and Kadowaki, Syusaku and Mukai, Yukinori (2016) Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae. In: 2nd International Conference on Oceanography and Sustainable Marine Production (ICOSMaP 2016), 23rd-25th Aug. 2016, Kuantan, Pahang. (Unpublished) http://www.iium.edu.my/icosmap/2016/wp-content/uploads/2016/08/ICOSMaP-2016.pdf |
| spellingShingle | SH Aquaculture. Fisheries. Angling Waludin, Shaidatul Nadia Afand, Muhammad Mu’izzuddin Mohammad Noor, Normawaty Phang, Ing Chia Kadowaki, Syusaku Mukai, Yukinori Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae |
| title | Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae |
| title_full | Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae |
| title_fullStr | Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae |
| title_full_unstemmed | Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae |
| title_short | Morphological and 18s rRNA sequencing of Euplotes sp.: a potential live feed for marine finfish larvae |
| title_sort | morphological and 18s rrna sequencing of euplotes sp.: a potential live feed for marine finfish larvae |
| topic | SH Aquaculture. Fisheries. Angling |
| url | http://irep.iium.edu.my/52404/ http://irep.iium.edu.my/52404/ http://irep.iium.edu.my/52404/1/52404_MORPHOLOGICAL%20AND%2018S%20rRNA.pdf |