Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia
Trichomonas vaginalis, a flagellated protozoan parasite is commonly found in the genitourinary tract. Binary fission is the only plausible mode of reproduction shown thus far but this does not account for the rapid growth rates seen in in vitro cultures within a short period of time. We have previ...
| Main Authors: | , |
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| Format: | Proceeding Paper |
| Language: | English English |
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2013
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| Online Access: | http://irep.iium.edu.my/52043/ http://irep.iium.edu.my/52043/1/Morphological%20aspects%20of%20multi-nucleated%20Trichomonas%20vaginalis%20from%20cervical%20cancer%20patients%20in%20Malaysia.pdf http://irep.iium.edu.my/52043/7/52043-program.pdf |
| _version_ | 1848783984596615168 |
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| author | Mat Yusof, Afzan Kumar, Suresh |
| author_facet | Mat Yusof, Afzan Kumar, Suresh |
| author_sort | Mat Yusof, Afzan |
| building | IIUM Repository |
| collection | Online Access |
| description | Trichomonas vaginalis, a flagellated protozoan parasite is commonly found in the genitourinary tract. Binary
fission is the only plausible mode of reproduction shown thus far but this does not account for the rapid growth
rates seen in in vitro cultures within a short period of time. We have previously shown ultrastructural evidence of
another asexual mode of reproduction other than binary fission seen in Trichmonas vaginalis. The present study
compares the number of multi-nucleated forms isolated from cervical neoplasia (CN) and non-cervical neoplasia
(NCN) patients. The present study highlights the detailed characterization of the morphological aspects of 4 CN
and 4 NCN isolates by using fixed staining and fluorescent staining of nuclear content. A sequential
transformation highlighting nuclear division leading to the formation of multi-nucleated parasite is shown. We
observe that this mode of reproduction is not commonly seen and postulate that it could be triggered to increase
progeny formation due to stress. These forms were viable when tested with trypan blue dye test. The study
confirms that the parasite divides by an asexual mode of reproduction other than binary fission and the parasites
isolated from cervical neoplasia showing larger numbers of nuclei accounting for higher parasite numbers in
cultures. |
| first_indexed | 2025-11-14T16:30:02Z |
| format | Proceeding Paper |
| id | iium-52043 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:30:02Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-520432016-10-05T02:25:36Z http://irep.iium.edu.my/52043/ Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia Mat Yusof, Afzan Kumar, Suresh Q Science (General) RC254 Neoplasms. Tumors. Oncology (including Cancer) Trichomonas vaginalis, a flagellated protozoan parasite is commonly found in the genitourinary tract. Binary fission is the only plausible mode of reproduction shown thus far but this does not account for the rapid growth rates seen in in vitro cultures within a short period of time. We have previously shown ultrastructural evidence of another asexual mode of reproduction other than binary fission seen in Trichmonas vaginalis. The present study compares the number of multi-nucleated forms isolated from cervical neoplasia (CN) and non-cervical neoplasia (NCN) patients. The present study highlights the detailed characterization of the morphological aspects of 4 CN and 4 NCN isolates by using fixed staining and fluorescent staining of nuclear content. A sequential transformation highlighting nuclear division leading to the formation of multi-nucleated parasite is shown. We observe that this mode of reproduction is not commonly seen and postulate that it could be triggered to increase progeny formation due to stress. These forms were viable when tested with trypan blue dye test. The study confirms that the parasite divides by an asexual mode of reproduction other than binary fission and the parasites isolated from cervical neoplasia showing larger numbers of nuclei accounting for higher parasite numbers in cultures. 2013 Proceeding Paper NonPeerReviewed application/pdf en http://irep.iium.edu.my/52043/1/Morphological%20aspects%20of%20multi-nucleated%20Trichomonas%20vaginalis%20from%20cervical%20cancer%20patients%20in%20Malaysia.pdf application/pdf en http://irep.iium.edu.my/52043/7/52043-program.pdf Mat Yusof, Afzan and Kumar, Suresh (2013) Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia. In: Biomicroworld2013 : V International Conference on Environmental , Industrial and Applied Microbiology, 2nd-4th October 2013, Madrid Spain. (Unpublished) http://www.biomicroworld2013.org/index.html |
| spellingShingle | Q Science (General) RC254 Neoplasms. Tumors. Oncology (including Cancer) Mat Yusof, Afzan Kumar, Suresh Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia |
| title | Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia |
| title_full | Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia |
| title_fullStr | Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia |
| title_full_unstemmed | Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia |
| title_short | Morphological aspects of multi-nucleated Trichomonas vaginalis from cervical cancer patients in Malaysia |
| title_sort | morphological aspects of multi-nucleated trichomonas vaginalis from cervical cancer patients in malaysia |
| topic | Q Science (General) RC254 Neoplasms. Tumors. Oncology (including Cancer) |
| url | http://irep.iium.edu.my/52043/ http://irep.iium.edu.my/52043/ http://irep.iium.edu.my/52043/1/Morphological%20aspects%20of%20multi-nucleated%20Trichomonas%20vaginalis%20from%20cervical%20cancer%20patients%20in%20Malaysia.pdf http://irep.iium.edu.my/52043/7/52043-program.pdf |