Tualang honey improves non alcoholic steatohepatitis animal model
Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1-2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH animal...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
2016
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| Online Access: | http://irep.iium.edu.my/51857/ http://irep.iium.edu.my/51857/42/51857.pdf |
| _version_ | 1848783948981731328 |
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| author | Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aeshah @ Naznin Abdul Rahim, Roslina |
| author_facet | Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aeshah @ Naznin Abdul Rahim, Roslina |
| author_sort | Abdul Ghani, Azril Shahreez |
| building | IIUM Repository |
| collection | Online Access |
| description | Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1-2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH animal model utilizing 12% cholesterol diet (CD) and to investigate the effects of Tualang honey (TH) known for its anti-inflammatory and anti-oxidative properties in this model. Methods: Twenty-four Sprague-Dawley rats were divided into 2 groups (12% CD and standard diet) and were fed for 6 weeks. Following the establishment of NASH, the rats in the 12% CD group were subsequently divided into 3 groups. The first group was continued with only 12% CD. In the other 2 groups in addition to the 12% CD they were given TH treatment at different concentrations (1.2 and 2.4 g/kg/day) for 4 weeks. Blood biochemical analysis and histological assessment of liver were subsequently performed. Results: The liver histological sections of the rats fed with 12% CD showed macrovesicular steatosis, ballooning degeneration with lobular and portal inflammation. They also had increased serum alanine aminotransferase (ALT), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting insulin, HOMA-IR and reduced high density lipoprotein cholesterol (HDL-C). Meanwhile, the TH treatment groups exhibited significant improvement in both the NASH grading and activity scores. The ALT, LDL-C, TC, triglyceride (TG), fasting insulin and HOMA-IR levels were reduced significantly. Conclusions: The 12% CD was able to induce NASH in the animal model. Tualang honey improved insulin sensitivity, dyslipidaemia, steatohepatitis. |
| first_indexed | 2025-11-14T16:29:28Z |
| format | Proceeding Paper |
| id | iium-51857 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:29:28Z |
| publishDate | 2016 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-518572022-04-07T01:57:35Z http://irep.iium.edu.my/51857/ Tualang honey improves non alcoholic steatohepatitis animal model Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aeshah @ Naznin Abdul Rahim, Roslina QP Physiology R Medicine (General) RA Public aspects of medicine RB Pathology RC Internal medicine RD Surgery Introduction: Non-alcoholic Steatohepatitis (NASH) is an emerging chronic liver disease with limited therapy available. Studies utilizing animal models induced with cholesterol diet ranging from 1-2% are hampered by inconsistent yield of NASH features. Therefore, we aimed to establish a NASH animal model utilizing 12% cholesterol diet (CD) and to investigate the effects of Tualang honey (TH) known for its anti-inflammatory and anti-oxidative properties in this model. Methods: Twenty-four Sprague-Dawley rats were divided into 2 groups (12% CD and standard diet) and were fed for 6 weeks. Following the establishment of NASH, the rats in the 12% CD group were subsequently divided into 3 groups. The first group was continued with only 12% CD. In the other 2 groups in addition to the 12% CD they were given TH treatment at different concentrations (1.2 and 2.4 g/kg/day) for 4 weeks. Blood biochemical analysis and histological assessment of liver were subsequently performed. Results: The liver histological sections of the rats fed with 12% CD showed macrovesicular steatosis, ballooning degeneration with lobular and portal inflammation. They also had increased serum alanine aminotransferase (ALT), total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), fasting insulin, HOMA-IR and reduced high density lipoprotein cholesterol (HDL-C). Meanwhile, the TH treatment groups exhibited significant improvement in both the NASH grading and activity scores. The ALT, LDL-C, TC, triglyceride (TG), fasting insulin and HOMA-IR levels were reduced significantly. Conclusions: The 12% CD was able to induce NASH in the animal model. Tualang honey improved insulin sensitivity, dyslipidaemia, steatohepatitis. 2016-08 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/51857/42/51857.pdf Abdul Ghani, Azril Shahreez and Abdullah, Nor Zamzila and Muhammad, Siti Aeshah @ Naznin and Abdul Rahim, Roslina (2016) Tualang honey improves non alcoholic steatohepatitis animal model. In: Medical Research Symposium & Pacific Partnership in conjunction with Kuantan Research Day 2016, 3rd-5th August 2016, Kuantan, Pahang, Malaysia. (Unpublished) |
| spellingShingle | QP Physiology R Medicine (General) RA Public aspects of medicine RB Pathology RC Internal medicine RD Surgery Abdul Ghani, Azril Shahreez Abdullah, Nor Zamzila Muhammad, Siti Aeshah @ Naznin Abdul Rahim, Roslina Tualang honey improves non alcoholic steatohepatitis animal model |
| title | Tualang honey improves non alcoholic steatohepatitis animal model |
| title_full | Tualang honey improves non alcoholic steatohepatitis animal model |
| title_fullStr | Tualang honey improves non alcoholic steatohepatitis animal model |
| title_full_unstemmed | Tualang honey improves non alcoholic steatohepatitis animal model |
| title_short | Tualang honey improves non alcoholic steatohepatitis animal model |
| title_sort | tualang honey improves non alcoholic steatohepatitis animal model |
| topic | QP Physiology R Medicine (General) RA Public aspects of medicine RB Pathology RC Internal medicine RD Surgery |
| url | http://irep.iium.edu.my/51857/ http://irep.iium.edu.my/51857/42/51857.pdf |