Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio
Many studies have been done on various species of insects to investigate their potential use in industries. This is because insects have high protein content which could be further manipulated. Due to its eating habit, Zophobas morio larvae, also known as supermeal worm has been shown to have high...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/51744/ http://irep.iium.edu.my/51744/1/51744.pdf |
| _version_ | 1848783922290229248 |
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| author | Easa, Muhammad Noor Yusof, Faridah Abd Halim, Amanatuzzakiah |
| author_facet | Easa, Muhammad Noor Yusof, Faridah Abd Halim, Amanatuzzakiah |
| author_sort | Easa, Muhammad Noor |
| building | IIUM Repository |
| collection | Online Access |
| description | Many studies have been done on various species of insects to investigate their potential use in industries. This
is because insects have high protein content which could be further manipulated. Due to its eating habit,
Zophobas morio larvae, also known as supermeal worm has been shown to have high amylase activity. In this
study, amylase from supermeal worm has been immobilized via Cross-Linked Enzyme Aggregates (CLEA)
technique and its kinetic performance, evaluated. CLEA is one of the best immobilization technology in
respect to enzyme stability and reusability. Kinetic performance of both free and CLEA-amylase were
evaluated based on the Michaelis-Menten model. Results obtained based Hanes-Woolf, Lineweaver-Burk,
Eadie-Hofstee and Hyperbolic Regression plots, showed that the kinetic parameters, Vmax and KM, changed
upon immobilization. For CLEA-amylase, Hanes-Woolf plot had showed the best fitted model based on R2
with Vmax= 1.068 mM/min and KM= 0.182 mM. Lineweaver-Burk plot was used to obtain the kinetic
parameters for free amylase, with Vmax and KM of 17.230 mM/min and 2.470 mM, respectively. The result
from this study might open the new path in discovering the potential of using insects in industrial
applications, for example, in detergent industry |
| first_indexed | 2025-11-14T16:29:03Z |
| format | Proceeding Paper |
| id | iium-51744 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:29:03Z |
| publishDate | 2016 |
| publisher | Kulliyah of Engineering, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-517442016-08-22T06:06:47Z http://irep.iium.edu.my/51744/ Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio Easa, Muhammad Noor Yusof, Faridah Abd Halim, Amanatuzzakiah QP Physiology TP248.13 Biotechnology Many studies have been done on various species of insects to investigate their potential use in industries. This is because insects have high protein content which could be further manipulated. Due to its eating habit, Zophobas morio larvae, also known as supermeal worm has been shown to have high amylase activity. In this study, amylase from supermeal worm has been immobilized via Cross-Linked Enzyme Aggregates (CLEA) technique and its kinetic performance, evaluated. CLEA is one of the best immobilization technology in respect to enzyme stability and reusability. Kinetic performance of both free and CLEA-amylase were evaluated based on the Michaelis-Menten model. Results obtained based Hanes-Woolf, Lineweaver-Burk, Eadie-Hofstee and Hyperbolic Regression plots, showed that the kinetic parameters, Vmax and KM, changed upon immobilization. For CLEA-amylase, Hanes-Woolf plot had showed the best fitted model based on R2 with Vmax= 1.068 mM/min and KM= 0.182 mM. Lineweaver-Burk plot was used to obtain the kinetic parameters for free amylase, with Vmax and KM of 17.230 mM/min and 2.470 mM, respectively. The result from this study might open the new path in discovering the potential of using insects in industrial applications, for example, in detergent industry Kulliyah of Engineering, International Islamic University Malaysia 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/51744/1/51744.pdf Easa, Muhammad Noor and Yusof, Faridah and Abd Halim, Amanatuzzakiah (2016) Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/ |
| spellingShingle | QP Physiology TP248.13 Biotechnology Easa, Muhammad Noor Yusof, Faridah Abd Halim, Amanatuzzakiah Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio |
| title | Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio |
| title_full | Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio |
| title_fullStr | Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio |
| title_full_unstemmed | Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio |
| title_short | Kinetic performance of cross-linked enzyme aggregates (clea)-amylase from Zophobas morio |
| title_sort | kinetic performance of cross-linked enzyme aggregates (clea)-amylase from zophobas morio |
| topic | QP Physiology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/51744/ http://irep.iium.edu.my/51744/ http://irep.iium.edu.my/51744/1/51744.pdf |