An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response
Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopte...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51732/ http://irep.iium.edu.my/51732/1/51732.pdf |
| _version_ | 1848783918428323840 |
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| author | Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia |
| author_facet | Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia |
| author_sort | Faruck, Mohammad Omer |
| building | IIUM Repository |
| collection | Online Access |
| description | Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius)
by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response
Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted to optimize the
extraction process parameters. The effect of independent parameters, namely, the homogenization
temperature (°C), homogenization time (min) and solid (g) to solvent (ml) ratio of the extraction process on
the fungal growth was studied. The extracted samples showed varying degree of antifungal activity against
Aspergillus niger, the selected fungal strain, by using poisoned agar assay technique. Response surface
investigation showed that the optimum values of the extraction parameters for antifungal peptides were 5
minutes homogenization time, 4°C homogenization temperature and 3.5:1 solid to solvent ratio. This study
reports the development of an extraction process that allows careful recovery of antifungal peptides from
insect larvae. In the validation of the experimental model, the error between the actual value and the
predicted value was determined to be 3.54%. |
| first_indexed | 2025-11-14T16:28:59Z |
| format | Proceeding Paper |
| id | iium-51732 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:28:59Z |
| publishDate | 2016 |
| publisher | Kulliyah of Engineering, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-517322016-08-22T01:31:04Z http://irep.iium.edu.my/51732/ An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia QP Physiology TP248.13 Biotechnology Antifungal peptides have been successfully extracted from whole body larvae of Zophobas morio (Fabricius) by using acidified isopropanol. To ensure that the extraction is cost effective for maximum yield, Response Surface Methodology (RSM) using a Central Composite Design (CCD) strategy was adopted to optimize the extraction process parameters. The effect of independent parameters, namely, the homogenization temperature (°C), homogenization time (min) and solid (g) to solvent (ml) ratio of the extraction process on the fungal growth was studied. The extracted samples showed varying degree of antifungal activity against Aspergillus niger, the selected fungal strain, by using poisoned agar assay technique. Response surface investigation showed that the optimum values of the extraction parameters for antifungal peptides were 5 minutes homogenization time, 4°C homogenization temperature and 3.5:1 solid to solvent ratio. This study reports the development of an extraction process that allows careful recovery of antifungal peptides from insect larvae. In the validation of the experimental model, the error between the actual value and the predicted value was determined to be 3.54%. Kulliyah of Engineering, International Islamic University Malaysia 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/51732/1/51732.pdf Faruck, Mohammad Omer and Yusof, Faridah and Chowdhury, Silvia (2016) An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/ |
| spellingShingle | QP Physiology TP248.13 Biotechnology Faruck, Mohammad Omer Yusof, Faridah Chowdhury, Silvia An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response |
| title | An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response |
| title_full | An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response |
| title_fullStr | An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response |
| title_full_unstemmed | An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response |
| title_short | An approach to optimize process parameter for peptides extraction from Zophobas morio (Fabricius) using antifungal activity as the response |
| title_sort | approach to optimize process parameter for peptides extraction from zophobas morio (fabricius) using antifungal activity as the response |
| topic | QP Physiology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/51732/ http://irep.iium.edu.my/51732/ http://irep.iium.edu.my/51732/1/51732.pdf |