Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond
Glycoscience is a field that explores the structures and functions of glycans promising great advances in an array of applications. The objective of this research is to form a sulfur-phosphorus bond at the position 6’ of the sugar involved in many biological processes. A directed evolution strateg...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51693/ http://irep.iium.edu.my/51693/1/51693.pdf |
| _version_ | 1848783910349045760 |
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| author | Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah |
| author_facet | Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah |
| author_sort | Bellag, Oualid Abdelkader |
| building | IIUM Repository |
| collection | Online Access |
| description | Glycoscience is a field that explores the structures and functions of glycans promising great advances in an
array of applications. The objective of this research is to form a sulfur-phosphorus bond at the position 6’ of
the sugar involved in many biological processes. A directed evolution strategy was adopted with error-prone
PCR technique to create random mutagenesis of recombinant plasmid library of the β-glucoside kinase (βglk)
recloned into a pTrcHisB plasmid and transformed into Escherichia coli. Following the creation of libraries,
high throughput screening by 96-well microplate using fluorescent activated cell sorting (FACS) techniques
were carried out. Positive clones were selected, sequenced for identification and enzyme expression
and characterization were carried out. |
| first_indexed | 2025-11-14T16:28:51Z |
| format | Proceeding Paper |
| id | iium-51693 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:28:51Z |
| publishDate | 2016 |
| publisher | Kulliyah of Engineering, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-516932016-08-19T02:05:15Z http://irep.iium.edu.my/51693/ Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah TP248.13 Biotechnology Glycoscience is a field that explores the structures and functions of glycans promising great advances in an array of applications. The objective of this research is to form a sulfur-phosphorus bond at the position 6’ of the sugar involved in many biological processes. A directed evolution strategy was adopted with error-prone PCR technique to create random mutagenesis of recombinant plasmid library of the β-glucoside kinase (βglk) recloned into a pTrcHisB plasmid and transformed into Escherichia coli. Following the creation of libraries, high throughput screening by 96-well microplate using fluorescent activated cell sorting (FACS) techniques were carried out. Positive clones were selected, sequenced for identification and enzyme expression and characterization were carried out. Kulliyah of Engineering, International Islamic University Malaysia 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/51693/1/51693.pdf Bellag, Oualid Abdelkader and Ben Belgacem, Farah Fadwa and Noor Batcha, Ibrahim Ali and Mohd Salleh, Hamzah (2016) Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/index.php/78-the-4th-international-conference-on-biotechnology-engineering |
| spellingShingle | TP248.13 Biotechnology Bellag, Oualid Abdelkader Ben Belgacem, Farah Fadwa Noor Batcha, Ibrahim Ali Mohd Salleh, Hamzah Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond |
| title | Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond |
| title_full | Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond |
| title_fullStr | Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond |
| title_full_unstemmed | Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond |
| title_short | Screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond |
| title_sort | screening of β-glucoside kinase directed evolution library to form a sulfur-phosphorus bond |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/51693/ http://irep.iium.edu.my/51693/ http://irep.iium.edu.my/51693/1/51693.pdf |