Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy
Green technology is the environment-friendly way to protect the Earth’s natural resources and the environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass...
| Main Authors: | , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyah of Engineering, International Islamic University Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/1/51691.pdf |
| _version_ | 1848783909681102848 |
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| author | Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah |
| author_facet | Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah |
| author_sort | Ben Belgacem, Farah Fadwa |
| building | IIUM Repository |
| collection | Online Access |
| description | Green technology is the environment-friendly way to protect the Earth’s natural resources and the
environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify
novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass through
metagenomic DNA library construction and high-throughput screening. Culture enrichment strategies are
additional pre-screening methods to provide an attractive mean of enhancing the screening hit rate. In this
work metagenomic DNA libraries were made from Malaysian palm oil mill effluent (POME) microorganisms
after culture enrichment experiment based on natural selection principle. Metagenomic DNA was extracted
from both enriched and non-enriched cultures and cloned to pCC1FOS fosmid and transformed into
EPI300™-T1RE. coli.Qualitative and quantitative analysis of metagenomic DNA and recombinant clones are
provided to confirm the efficiency of enrichment strategy. |
| first_indexed | 2025-11-14T16:28:51Z |
| format | Proceeding Paper |
| id | iium-51691 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:28:51Z |
| publishDate | 2016 |
| publisher | Kulliyah of Engineering, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-516912016-08-19T01:50:07Z http://irep.iium.edu.my/51691/ Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah TP248.13 Biotechnology Green technology is the environment-friendly way to protect the Earth’s natural resources and the environment we inhabit. The functionalmetagenomic approach has proven to be a powerful tool to identify novel biocatalysts indispensables in green technology of biofuel production from plant’s biomass through metagenomic DNA library construction and high-throughput screening. Culture enrichment strategies are additional pre-screening methods to provide an attractive mean of enhancing the screening hit rate. In this work metagenomic DNA libraries were made from Malaysian palm oil mill effluent (POME) microorganisms after culture enrichment experiment based on natural selection principle. Metagenomic DNA was extracted from both enriched and non-enriched cultures and cloned to pCC1FOS fosmid and transformed into EPI300™-T1RE. coli.Qualitative and quantitative analysis of metagenomic DNA and recombinant clones are provided to confirm the efficiency of enrichment strategy. Kulliyah of Engineering, International Islamic University Malaysia 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/51691/1/51691.pdf Ben Belgacem, Farah Fadwa and Bellag, Oualid Abdel Kader and Noor Batcha, Ibrahim Ali and Mohd Salleh , Hamzah (2016) Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/index.php/78-the-4th-international-conference-on-biotechnology-engineering |
| spellingShingle | TP248.13 Biotechnology Ben Belgacem, Farah Fadwa Bellag, Oualid Abdel Kader Noor Batcha, Ibrahim Ali Mohd Salleh , Hamzah Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy |
| title | Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy |
| title_full | Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy |
| title_fullStr | Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy |
| title_full_unstemmed | Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy |
| title_short | Bioprospecting novel cellulose-degrading enzymes from pome metagenomic DNA libraries by enrichment strategy |
| title_sort | bioprospecting novel cellulose-degrading enzymes from pome metagenomic dna libraries by enrichment strategy |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/ http://irep.iium.edu.my/51691/1/51691.pdf |