Statistical optimization of gelatin immobilization to improve PCL microcarrier surface
Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent...
| Main Authors: | , , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
| Published: |
Kulliyyah of Engineering, International Islamic University Malaysia
2016
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/51646/ http://irep.iium.edu.my/51646/1/51646_Statistical_optimization_of_gelatin.pdf |
| _version_ | 1848783896919932928 |
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| author | Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini |
| author_facet | Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini |
| author_sort | Samsudin, Nurhusna |
| building | IIUM Repository |
| collection | Online Access |
| description | Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, oxygen functional group was introduced via ultraviolet irradiation and ozone aeration (UV/O3) system to allow covalent immobilization of gelatin on the PCL microcarrier surface using zero-length crosslinker reagent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide (EDAC/NHS). The optimum conditions for maximizing the amount of gelatin on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10mM NHS concentration and, 80 mg/ml gelatin. |
| first_indexed | 2025-11-14T16:28:39Z |
| format | Proceeding Paper |
| id | iium-51646 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:28:39Z |
| publishDate | 2016 |
| publisher | Kulliyyah of Engineering, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-516462017-10-19T06:41:32Z http://irep.iium.edu.my/51646/ Statistical optimization of gelatin immobilization to improve PCL microcarrier surface Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini Q Science (General) Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the main challenge remains at ensuring the surface biocompatibility with cells. Polycaprolactone (PCL), a biodegradable polymer, has received considerable attention because of its excellent mechanical properties and degradation kinetics that suit various applications, but its non-polar hydrocarbon moiety renders it sub-optimal for cell attachment. In this present study, oxygen functional group was introduced via ultraviolet irradiation and ozone aeration (UV/O3) system to allow covalent immobilization of gelatin on the PCL microcarrier surface using zero-length crosslinker reagent 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysuccinimide (EDAC/NHS). The optimum conditions for maximizing the amount of gelatin on the surface of PCL were as follows: 1.5 of COOH:EDAC ratio, 10mM NHS concentration and, 80 mg/ml gelatin. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/51646/1/51646_Statistical_optimization_of_gelatin.pdf Samsudin, Nurhusna and Hashim, Yumi Zuhanis Has-Yun and Arifin, Mohd Azmir and Mel, Maizirwan and Mohd. Salleh, Hamzah and Sopyan, Iis and Jimat, Dzun Noraini (2016) Statistical optimization of gelatin immobilization to improve PCL microcarrier surface. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/ |
| spellingShingle | Q Science (General) Samsudin, Nurhusna Hashim, Yumi Zuhanis Has-Yun Arifin, Mohd Azmir Mel, Maizirwan Mohd. Salleh, Hamzah Sopyan, Iis Jimat, Dzun Noraini Statistical optimization of gelatin immobilization to improve PCL microcarrier surface |
| title | Statistical optimization of gelatin immobilization to improve PCL microcarrier surface |
| title_full | Statistical optimization of gelatin immobilization to improve PCL microcarrier surface |
| title_fullStr | Statistical optimization of gelatin immobilization to improve PCL microcarrier surface |
| title_full_unstemmed | Statistical optimization of gelatin immobilization to improve PCL microcarrier surface |
| title_short | Statistical optimization of gelatin immobilization to improve PCL microcarrier surface |
| title_sort | statistical optimization of gelatin immobilization to improve pcl microcarrier surface |
| topic | Q Science (General) |
| url | http://irep.iium.edu.my/51646/ http://irep.iium.edu.my/51646/ http://irep.iium.edu.my/51646/1/51646_Statistical_optimization_of_gelatin.pdf |