Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum
Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees, rich in soluble dietary fibers and organic compounds that appear to be capable of preventing many diseases. This study allowed for quantitation of the levels of total phenolic cont...
| Main Authors: | , , , , , |
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| Format: | Proceeding Paper |
| Language: | English |
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Kulliyyah of Engineering, International Islamic University Malaysia
2016
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| Online Access: | http://irep.iium.edu.my/50917/ http://irep.iium.edu.my/50917/1/50917_Effect_of_solvents_extraction.pdf |
| _version_ | 1848783748888264704 |
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| author | Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid |
| author_facet | Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid |
| author_sort | Mohammedelnour, Ahmed A. |
| building | IIUM Repository |
| collection | Online Access |
| description | Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees, rich in soluble dietary fibers and organic compounds that appear to be capable of preventing many diseases. This study allowed for quantitation of the levels of total phenolic content (TPC) and antioxidant activity using ABTS and CUPRAC assays from APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation decolourisation and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. As results, the solvent methanol (50%) gave the best extraction rates for APG presented by highest TPC (60.7833 ± 0.0416), CUPRAC (34.6533± 0.0611 mg TF/ 100g) and ABTS (34.6533± 0.0611 mg TF/ 100g) followed by ethanol 50%. On the other hand, pure methanol showed the lowest TPC (05.3300 ± 0.0608 mg GAE /100g DW), ABTS (10.9000 ± 0.0436 mg TF/ 100g), and CUPRAC (7.8000± 0.1249 mg TF/ 100g) values. Therefore, the variation in the antioxidant capacity between extracts was probably due to the differences in the nature of the APG compounds extracted using different solvents. The higher content of antioxidant activity in APG may have beneficial effects on the human health. |
| first_indexed | 2025-11-14T16:26:17Z |
| format | Proceeding Paper |
| id | iium-50917 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:26:17Z |
| publishDate | 2016 |
| publisher | Kulliyyah of Engineering, International Islamic University Malaysia |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-509172016-11-04T07:43:15Z http://irep.iium.edu.my/50917/ Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Acacia polyacantha gum (APG) is a dried exudate which obtained from the stems and branches of Acacia polyacantha trees, rich in soluble dietary fibers and organic compounds that appear to be capable of preventing many diseases. This study allowed for quantitation of the levels of total phenolic content (TPC) and antioxidant activity using ABTS and CUPRAC assays from APG extraction using pure solvents (methanol, ethanol, acetone) and their aqueous mixtures at 50% and 100%. The antioxidant levels were evaluated by 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS+) radical cation decolourisation and cupric iron reducing capacity in the presence of neocuproine (CUPRAC) for the evaluation of reducing power, and (TPC) was evaluated by the Folin-Ciocalteu method. As results, the solvent methanol (50%) gave the best extraction rates for APG presented by highest TPC (60.7833 ± 0.0416), CUPRAC (34.6533± 0.0611 mg TF/ 100g) and ABTS (34.6533± 0.0611 mg TF/ 100g) followed by ethanol 50%. On the other hand, pure methanol showed the lowest TPC (05.3300 ± 0.0608 mg GAE /100g DW), ABTS (10.9000 ± 0.0436 mg TF/ 100g), and CUPRAC (7.8000± 0.1249 mg TF/ 100g) values. Therefore, the variation in the antioxidant capacity between extracts was probably due to the differences in the nature of the APG compounds extracted using different solvents. The higher content of antioxidant activity in APG may have beneficial effects on the human health. Kulliyyah of Engineering, International Islamic University Malaysia 2016 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/50917/1/50917_Effect_of_solvents_extraction.pdf Mohammedelnour, Ahmed A. and Mirghani, Mohamed Elwathig Saeed and Kabbashi, Nassereldeen Ahmed and Alam, Md. Zahangir and Abdullah, Amina and Musa, Khalid Hamid (2016) Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum. In: 4th International Conference on Biotechnology Engineering 2016 (ICBioE 2016), 25th-27th July 2016, Kuala Lumpur. http://www.iium.edu.my/icbioe/2016/ |
| spellingShingle | TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices Mohammedelnour, Ahmed A. Mirghani, Mohamed Elwathig Saeed Kabbashi, Nassereldeen Ahmed Alam, Md. Zahangir Abdullah, Amina Musa, Khalid Hamid Effect of solvents extraction on the antioxidant activity of Acacia polyacantha gum |
| title | Effect of solvents extraction on the antioxidant activity
of Acacia polyacantha gum |
| title_full | Effect of solvents extraction on the antioxidant activity
of Acacia polyacantha gum |
| title_fullStr | Effect of solvents extraction on the antioxidant activity
of Acacia polyacantha gum |
| title_full_unstemmed | Effect of solvents extraction on the antioxidant activity
of Acacia polyacantha gum |
| title_short | Effect of solvents extraction on the antioxidant activity
of Acacia polyacantha gum |
| title_sort | effect of solvents extraction on the antioxidant activity
of acacia polyacantha gum |
| topic | TK7800 Electronics. Computer engineering. Computer hardware. Photoelectronic devices |
| url | http://irep.iium.edu.my/50917/ http://irep.iium.edu.my/50917/ http://irep.iium.edu.my/50917/1/50917_Effect_of_solvents_extraction.pdf |