Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor
A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (Phanerochaete chrysosporium) using sewage-treatmentplant (STP) sludge as the major substrate. The optimization was done using full-factorial design (FFD) with agitation and aeration as the two para...
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| Format: | Article |
| Language: | English |
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Springer
2009
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| Online Access: | http://irep.iium.edu.my/5065/ http://irep.iium.edu.my/5065/1/Paper-LiP-JIMB.pdf |
| _version_ | 1848776441798328320 |
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| author | Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal |
| author_facet | Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal |
| author_sort | Alam, Md. Zahangir |
| building | IIUM Repository |
| collection | Online Access |
| description | A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (Phanerochaete chrysosporium) using sewage-treatmentplant (STP) sludge as the major substrate. The optimization was done using full-factorial design (FFD) with agitation and aeration as the two parameters. Nine experiments indicated by the FFD were fermented in a stirred-tank bioreactor for 3 days. A second-order quadratic model was developed using the regression analysis of the experimental results with the linear, quadratic, and interaction effects of
the parameters. Analysis of variance (ANOVA) showed a
high coefficient of determination (R2) value of 0.972, thus
indicating a satisfactory fit of the quadratic model with the experimental data. Using statistical analysis, the optimum aeration and agitation rates were determined to be 2.0 vvm and 200 rpm, respectively, with a maximum activity of 225 U l-1 in the first 3 days of fermentation. The validation experiment showed the maximum activity of lignin
peroxidase was 744 U l-1 after 5 days of fermentation. The results for the tests of the stability of lignin peroxidase
showed that the activity was more than 80% of the maximum
for the first 12 h of incubation at an optimum pH of 5
and temperature of 55�C. |
| first_indexed | 2025-11-14T14:30:09Z |
| format | Article |
| id | iium-5065 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T14:30:09Z |
| publishDate | 2009 |
| publisher | Springer |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-50652017-12-07T04:36:52Z http://irep.iium.edu.my/5065/ Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal QP Physiology TP Chemical technology TP248.13 Biotechnology A laboratory-scale study was carried out to produce lignin peroxidase (ligninase) by white rot fungus (Phanerochaete chrysosporium) using sewage-treatmentplant (STP) sludge as the major substrate. The optimization was done using full-factorial design (FFD) with agitation and aeration as the two parameters. Nine experiments indicated by the FFD were fermented in a stirred-tank bioreactor for 3 days. A second-order quadratic model was developed using the regression analysis of the experimental results with the linear, quadratic, and interaction effects of the parameters. Analysis of variance (ANOVA) showed a high coefficient of determination (R2) value of 0.972, thus indicating a satisfactory fit of the quadratic model with the experimental data. Using statistical analysis, the optimum aeration and agitation rates were determined to be 2.0 vvm and 200 rpm, respectively, with a maximum activity of 225 U l-1 in the first 3 days of fermentation. The validation experiment showed the maximum activity of lignin peroxidase was 744 U l-1 after 5 days of fermentation. The results for the tests of the stability of lignin peroxidase showed that the activity was more than 80% of the maximum for the first 12 h of incubation at an optimum pH of 5 and temperature of 55�C. Springer 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/5065/1/Paper-LiP-JIMB.pdf Alam, Md. Zahangir and Mansor, Mariatul F. and Khan Chowdhury, Ahmed Jalal (2009) Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor. Journal of Industrial Microbiology & Biotechnology, 36 (5). pp. 757-764. ISSN 1367-5435 http://dx.doi.org/10.1007/s10295-009-0548-5 doi:10.1007/s10295-009-0548-5 |
| spellingShingle | QP Physiology TP Chemical technology TP248.13 Biotechnology Alam, Md. Zahangir Mansor, Mariatul F. Khan Chowdhury, Ahmed Jalal Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title | Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_full | Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_fullStr | Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_full_unstemmed | Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_short | Optimization of lignin peroxidase production and stability by Phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| title_sort | optimization of lignin peroxidase production and stability by phanerochaete chrysosporium using sewage-treatment-plant sludge as substrate in a stirred-tank bioreactor |
| topic | QP Physiology TP Chemical technology TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/5065/ http://irep.iium.edu.my/5065/ http://irep.iium.edu.my/5065/ http://irep.iium.edu.my/5065/1/Paper-LiP-JIMB.pdf |