Bromelain enzyme assay using Casen as substrate

QR Code

Bromelain enzyme assay using Casen as substrate

This procedure is following Sigma's non-specific protease activity. In this assay, casein acts as a substrate to the reaction. When protease digest casein, the amino acid tyrosine is liberated along with other amino acids and peptide fragments. Folin & Ciocalteus Phenol, or Folin's rea...

Full description

Bibliographic Details
Main Authors:	Amid, Azura, Jamaluddin, Mohd Jamil Aizat
Format:	Book Chapter
Language:	English
Published:	IIUM Press 2016
Subjects:	TP248.13 Biotechnology
Online Access:	http://irep.iium.edu.my/49849/ http://irep.iium.edu.my/49849/1/49849_Bromelain_enzyme_assay_using_casein.pdf

Similar Items

Case study: recombinant Bromelain cloning, characterization and upstream processes
by: Amid, Azura, et al.
Published: (2015)

Effects of operating conditions in spray drying of recombinant bromelain
by: Amid, Azura, et al.
Published: (2014)

Determination of optimal range of post-induction temperature for production of soluble recombinant bromelain in Escherichia coli using one-factor-at-a-time (OFAT) approach
by: Amid, Azura, et al.
Published: (2011)

Optimization on cell disruption of E. coli BL21-AI expressing recombinant bromelain
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2014)

Process optimization on cell disruption of recombinant bromelain expressed in E. coli BL21-AI using ultrasonication
by: Othman, Muhd. Ezza Faiez, et al.
Published: (2013)

Screening of autoinduction medium composition for optimial biomass production of E.coli expressing stem bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2013)

Recombinant-enzyme fermentation
by: Amid, Azura, et al.
Published: (2015)

Functional characterization of recombinant bromelain
by: Amid, Azura, et al.
Published: (2015)

Screening of important autoinduction medium composition for high biaomass production of E. coli expressing recombinant bromelain
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2014)

Case Study: Recombinant Bromelain Selection
by: Amid, Azura, et al.
Published: (2015)

Observation on recombinant bromelain activity before and after purification
by: Ismail, Nurul Azira, et al.
Published: (2010)

Heterologous expression of Bromelain in Escherichia coli
by: M., Bala, et al.
Published: (2011)

Kinetic studies on recombinant stem bromelain
by: Bala, Muntari, et al.
Published: (2013)

Molecular cloning sequencing purification of stem bromelain from Ananas comosus
by: Amid, Azura, et al.
Published: (2010)

Case Study: Recombinant Bromelain Downstream Processing
by: Amid, Azura, et al.
Published: (2015)

Up-scaling cream cheese production by 100 l per day cow milk using bromelain as rennet alternative
by: Amid, Azura
Published: (2019)

Factors affecting enzyme assays
by: Mohd. Salleh, Hamzah
Published: (2011)

Bromelain: an overview of industrial application and purification strategies
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2014)

Effects of process parameters on spray drying of recombinant bromelain
by: Muntari, Bala, et al.
Published: (2013)

Effect of commercial bromelain on viability of MCF-7 human breast cancer cells
by: Yahaya, Hanan, et al.
Published: (2013)

Gene expression analysis in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Economic and environmental evaluation of recombinant enzyme production
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2015)

Comparison of cell disruption method for E. coli BL21-AI expressing recombinant bromelain
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)

Pathway analysis of genes affected in MCF-7 breast cancer cells treated with recombinant bromelain
by: Fouz, Nour, et al.
Published: (2014)

Introduction to recombinant enzyme pre-commecialization
by: Amid, Azura
Published: (2015)

Recombinant Enzyme: Cloning and Expression
by: Amid, Azura, et al.
Published: (2015)

Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2017)

Comparison of different cell disruption methods and cell extractant buffers for recombinant bromelain expressed in E.coli BL21-A1
by: Mohd Arshad, Zatul Iffah, et al.
Published: (2015)

Screening of potential strains and co-substrate for production of cellulase enzyme using sewage treatment plant sludge as major substrate
by: Drani , Afifah, et al.
Published: (2011)

Production of intracellular recombinant bromelain by Escherichia coli BL21-AI using shake flask and stirred tank bioreactor
by: Jamaluddin, Mohd Jamil Aizat, et al.
Published: (2017)

Purification and Cytotoxicity Assay of TOmato (Lycopersicum esculentum) Leaves Methanol Extract as Potential Anticancer Agent
by: Wan Chik, Wan Dalila, et al.
Published: (2010)

Common Laboratory Procedure
by: Amid, Azura, et al.
Published: (2015)

The factors affecting the extraction condition for neuroprotective activity of centella asiatica evaluated by metal chelating activity assay
by: Amid, Azura, et al.
Published: (2010)

Purification and cytotoxicity assay of tomato (Lycopersicon Esculentum) leaves methanol extract as potential anti-cancer agent
by: Wan Chik, Wan Dalila, et al.
Published: (2010)

Isolation of thermotolerant bacteria producing fibrinolytic enzyme
by: Ab. Shukor , Nurul Aqilah, et al.
Published: (2015)

Isolation of thermotolerant bacteria producing fibrinolytic enzyme
by: Ab Shukor, Nurul Aqilah, et al.
Published: (2015)

Methods for screening the potential natural compound for treating metabolic disorder diseases: anti-inflammatory and griess assay [Nitric Oxide (NO) measurement]
by: Amid, Azura, et al.
Published: (2011)

Gene expression analysis of fruit bromelain in ripening of Ananas comosus cultivar MD 2
by: Pang, Wei Cheng, et al.
Published: (2020)

Enzyme-aided extraction of sulfated polysaccharides from Turbinaria turbinata brown seaweed
by: Hammed, Ademola Monsur, et al.
Published: (2017)

Immobilization of enzymes
by: Yusof, Faridah
Published: (2011)