Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase
This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red...
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| Format: | Book Chapter |
| Language: | English |
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IIUM Press
2016
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| Online Access: | http://irep.iium.edu.my/49762/ http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf |
| _version_ | 1848783513916014592 |
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| author | Jimat, Dzun Noraini Mohamed , Intan Baizura Firda |
| author2 | Jamal, Parveen |
| author_facet | Jamal, Parveen Jimat, Dzun Noraini Mohamed , Intan Baizura Firda |
| author_sort | Jimat, Dzun Noraini |
| building | IIUM Repository |
| collection | Online Access |
| description | This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red which later shows an indication of L-asparaginase based on changes of colour. Phenol red at acidic is yellow which might change colour to pink when it is at alkaline. Thus, it indicates the formation of pink zone around microbial colonies producing L-asparaginase. The inoculated agar plates are incubated at 370C in an incubator for 24 hours. Thus, the isolation and screening of bacterial producing L-asparaginase are based on phenol-red zone. After purification, the isolates are characterised using several biochemical tests. |
| first_indexed | 2025-11-14T16:22:33Z |
| format | Book Chapter |
| id | iium-49762 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:22:33Z |
| publishDate | 2016 |
| publisher | IIUM Press |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-497622017-01-16T06:23:28Z http://irep.iium.edu.my/49762/ Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase Jimat, Dzun Noraini Mohamed , Intan Baizura Firda TP248.13 Biotechnology This procedure is following Gulati, Saxena, and Gupta (1997) protocol which is known as semi-quantitative plate assay for screening L-asparaginase- producing bacterial from local hot spring. The plate agar medium containing asparagine (as sole nitrogen sourse) is prepared with addition of phenol red which later shows an indication of L-asparaginase based on changes of colour. Phenol red at acidic is yellow which might change colour to pink when it is at alkaline. Thus, it indicates the formation of pink zone around microbial colonies producing L-asparaginase. The inoculated agar plates are incubated at 370C in an incubator for 24 hours. Thus, the isolation and screening of bacterial producing L-asparaginase are based on phenol-red zone. After purification, the isolates are characterised using several biochemical tests. IIUM Press Jamal, Parveen Azmi, Azlin Suhaida Ibrahim Ali , Noorbatcha 2016-01 Book Chapter NonPeerReviewed application/pdf en http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf Jimat, Dzun Noraini and Mohamed , Intan Baizura Firda (2016) Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase. In: Experimental Methods in Modern Biotechnology. IIUM Press Series of Textbook from the Islamic Perspectives, 2 . IIUM Press, Kuala Lumpur, pp. 72-80. ISBN 978-967-418-384-4 http://iiumpress.iium.edu.my/bookshop/experimental-methods-in-modern-biotechnology |
| spellingShingle | TP248.13 Biotechnology Jimat, Dzun Noraini Mohamed , Intan Baizura Firda Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase |
| title | Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase |
| title_full | Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase |
| title_fullStr | Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase |
| title_full_unstemmed | Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase |
| title_short | Isolation and characterisation of thermophilic bacterial isolates producing L-asparaginase |
| title_sort | isolation and characterisation of thermophilic bacterial isolates producing l-asparaginase |
| topic | TP248.13 Biotechnology |
| url | http://irep.iium.edu.my/49762/ http://irep.iium.edu.my/49762/ http://irep.iium.edu.my/49762/1/49762_Isolation_and_characterisation_of_thermophilic_bacterial_isolates_producing_L-asparaginase.pdf |