In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice
Background: Ancient traditional physicians from many countries used leeching to treat a wide range of diseases for thousands of years. A large number of peptides and proteins have been identified and characterized in leech saliva extract (LSE), including anti thrombotic agents, cancer metastasis...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society of Clinical Oncology
2015
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| Online Access: | http://irep.iium.edu.my/49434/ http://irep.iium.edu.my/49434/1/In%C2%A0vivo%C2%A0assessment%C2%A0of%C2%A0the%C2%A0therapeutic%C2%A0effects%C2%A0of%C2%A0lyophilized%C2%A0leech.pdf |
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| author | Ammar, Amr Hessein, Mohamed Guns, Emma Chin, Mei Helal Uddin, A.B.M. Abdualkader, Abdulrahman Alaama, Mohammed Merzouk, Ahmed Ghawi, Abbas Kucuk, Omer |
| author_facet | Ammar, Amr Hessein, Mohamed Guns, Emma Chin, Mei Helal Uddin, A.B.M. Abdualkader, Abdulrahman Alaama, Mohammed Merzouk, Ahmed Ghawi, Abbas Kucuk, Omer |
| author_sort | Ammar, Amr |
| building | IIUM Repository |
| collection | Online Access |
| description | Background: Ancient traditional physicians from many countries used leeching to treat a
wide range of diseases for thousands of years. A large number of peptides and proteins
have been identified and characterized in leech saliva extract (LSE), including anti
thrombotic agents, cancer metastasis inhibitors and antimicrobials Currently, leech therapy
is established as an important tool in microsurgery and reconstructive operations having
demonstrated superior clinical outcomes for the optimal salvage of grafted tissues.
Methods: In the current study, we have determined the in vivo efficacy of LSE from
(Huridinaria manillensis) on castration resistant LNCaP xenograft mouse model. Mice were
divided into three groups of six, mice were subcutaneously injected with either LSE (5
mg/kg), docetaxel (10 mg/kg), or vehicle once a week. PSA and tumor volume were
measured weekly. After four weeks of treatment, mice were euthanized, tumors and
organs were collected for transcriptome and immunohistochemical (IHC) analysis.
Results:
There was a significant decrease in the tumor volume and PSA with either docetaxel
(10mg/kg) or LSE (5 mg/kg) treated groups compared to the control. While there was no
significant difference between the antitumor activity of docetaxel (10mg/kg) and LSE (5
mg/kg). IHC showed significant increase in caspase3 and significant decrease in Ki67 and
PCNA expression in the LSE treated mice compared to the control group. Interestingly,
transcriptome analysis of tumor samples showed that LSE modulated cytokine production,
monocyte adhesion, steroidogenesis, and P38 MAPK signaling pathways. Conclusions:
LSE has significant antitumor activity in LNCaP tumor xenograft models with no apparent
side effects. This can be attributed, at least partly, to its inhibition of cellular proliferation,
induction of apoptosis, modulation of immunity and steroidogenesis.
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| first_indexed | 2025-11-14T16:21:39Z |
| format | Article |
| id | iium-49434 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English |
| last_indexed | 2025-11-14T16:21:39Z |
| publishDate | 2015 |
| publisher | American Society of Clinical Oncology |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-494342022-11-07T03:22:11Z http://irep.iium.edu.my/49434/ In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice Ammar, Amr Hessein, Mohamed Guns, Emma Chin, Mei Helal Uddin, A.B.M. Abdualkader, Abdulrahman Alaama, Mohammed Merzouk, Ahmed Ghawi, Abbas Kucuk, Omer RM Therapeutics. Pharmacology RS Pharmacy and materia medica Background: Ancient traditional physicians from many countries used leeching to treat a wide range of diseases for thousands of years. A large number of peptides and proteins have been identified and characterized in leech saliva extract (LSE), including anti thrombotic agents, cancer metastasis inhibitors and antimicrobials Currently, leech therapy is established as an important tool in microsurgery and reconstructive operations having demonstrated superior clinical outcomes for the optimal salvage of grafted tissues. Methods: In the current study, we have determined the in vivo efficacy of LSE from (Huridinaria manillensis) on castration resistant LNCaP xenograft mouse model. Mice were divided into three groups of six, mice were subcutaneously injected with either LSE (5 mg/kg), docetaxel (10 mg/kg), or vehicle once a week. PSA and tumor volume were measured weekly. After four weeks of treatment, mice were euthanized, tumors and organs were collected for transcriptome and immunohistochemical (IHC) analysis. Results: There was a significant decrease in the tumor volume and PSA with either docetaxel (10mg/kg) or LSE (5 mg/kg) treated groups compared to the control. While there was no significant difference between the antitumor activity of docetaxel (10mg/kg) and LSE (5 mg/kg). IHC showed significant increase in caspase3 and significant decrease in Ki67 and PCNA expression in the LSE treated mice compared to the control group. Interestingly, transcriptome analysis of tumor samples showed that LSE modulated cytokine production, monocyte adhesion, steroidogenesis, and P38 MAPK signaling pathways. Conclusions: LSE has significant antitumor activity in LNCaP tumor xenograft models with no apparent side effects. This can be attributed, at least partly, to its inhibition of cellular proliferation, induction of apoptosis, modulation of immunity and steroidogenesis. American Society of Clinical Oncology 2015-03 Article PeerReviewed application/pdf en http://irep.iium.edu.my/49434/1/In%C2%A0vivo%C2%A0assessment%C2%A0of%C2%A0the%C2%A0therapeutic%C2%A0effects%C2%A0of%C2%A0lyophilized%C2%A0leech.pdf Ammar, Amr and Hessein, Mohamed and Guns, Emma and Chin, Mei and Helal Uddin, A.B.M. and Abdualkader, Abdulrahman and Alaama, Mohammed and Merzouk, Ahmed and Ghawi, Abbas and Kucuk, Omer (2015) In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice. Journal of Clinical Oncology, 33 (7 (Supp.)). p. 271. ISSN 0732-183X E-ISSN 1527-7755 http://meeting.ascopubs.org/cgi/content/abstract/33/7_suppl/271?sid=2ea1a1d4-9eeb-4f02-8270-cfffe2db25a5 |
| spellingShingle | RM Therapeutics. Pharmacology RS Pharmacy and materia medica Ammar, Amr Hessein, Mohamed Guns, Emma Chin, Mei Helal Uddin, A.B.M. Abdualkader, Abdulrahman Alaama, Mohammed Merzouk, Ahmed Ghawi, Abbas Kucuk, Omer In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice |
| title | In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice |
| title_full | In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice |
| title_fullStr | In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice |
| title_full_unstemmed | In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice |
| title_short | In vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (Huridinaria manillensis) on LNCaP tumor xenograft model in nude mice |
| title_sort | in vivo assessment of the therapeutic effects of lyophilized leech saliva extract from (huridinaria manillensis) on lncap tumor xenograft model in nude mice |
| topic | RM Therapeutics. Pharmacology RS Pharmacy and materia medica |
| url | http://irep.iium.edu.my/49434/ http://irep.iium.edu.my/49434/ http://irep.iium.edu.my/49434/1/In%C2%A0vivo%C2%A0assessment%C2%A0of%C2%A0the%C2%A0therapeutic%C2%A0effects%C2%A0of%C2%A0lyophilized%C2%A0leech.pdf |