Performance of β-glucosidase immobilized on calcium alginate beads
Performance of immobilized β-glucosidase obtained from almonds (EC 3.2.1.21) on calcium alginate beads was studied by measuring the activity of the enzyme in terms of the generation of pnitrophenol from the hydrolysis of p-nitrophenyl-β-D-glucopyranoside (PNPG).The immobilized enzyme activity was c...
| Main Authors: | , , |
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| Format: | Proceeding Paper |
| Language: | English English |
| Published: |
2013
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/48525/ http://irep.iium.edu.my/48525/1/ICBIOE-2013-PERFORMANCE_OF_%CE%B2-GLUCOSIDASE_IMMOBILIZED_ON_CALCIUM.pdf http://irep.iium.edu.my/48525/4/1175_Acceptance.pdf |
| _version_ | 1848783312786554880 |
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| author | Jameel, Ahmed Tariq Yusof, Faridah Johana, Syaira |
| author_facet | Jameel, Ahmed Tariq Yusof, Faridah Johana, Syaira |
| author_sort | Jameel, Ahmed Tariq |
| building | IIUM Repository |
| collection | Online Access |
| description | Performance of immobilized β-glucosidase obtained from almonds (EC 3.2.1.21) on calcium alginate beads was studied by measuring the activity of the enzyme in terms of the generation of pnitrophenol from the hydrolysis of p-nitrophenyl-β-D-glucopyranoside (PNPG).The immobilized
enzyme activity was compared with soluble enzyme and was found to decrease by 36.6% albeit with an increased operational stability in terms of easy recovery from the finished product, recurrent use and scale-up in various reactor configurations. The hydrolysis rate data exhibit Michaelis-Menten kinetics. The Michaelis constant were determined using Langmuir linearized plot which
obtained vmax and Km as 20.88 μmol/mL.min and 0.0125 mol/L respectively. However, the immobilized β-Glucosidase did not show a sufficiently good operational stability on reuse.
Keywords: Immobilized enzyme, enzyme activity, Michaelis-Menten kinetics, β-glucosidase, pnitrophenyl-
β-D-glucopyranoside (PNPG), alginate gel |
| first_indexed | 2025-11-14T16:19:21Z |
| format | Proceeding Paper |
| id | iium-48525 |
| institution | International Islamic University Malaysia |
| institution_category | Local University |
| language | English English |
| last_indexed | 2025-11-14T16:19:21Z |
| publishDate | 2013 |
| recordtype | eprints |
| repository_type | Digital Repository |
| spelling | iium-485252018-05-05T18:09:19Z http://irep.iium.edu.my/48525/ Performance of β-glucosidase immobilized on calcium alginate beads Jameel, Ahmed Tariq Yusof, Faridah Johana, Syaira TP Chemical technology TP155 Chemical engineering Performance of immobilized β-glucosidase obtained from almonds (EC 3.2.1.21) on calcium alginate beads was studied by measuring the activity of the enzyme in terms of the generation of pnitrophenol from the hydrolysis of p-nitrophenyl-β-D-glucopyranoside (PNPG).The immobilized enzyme activity was compared with soluble enzyme and was found to decrease by 36.6% albeit with an increased operational stability in terms of easy recovery from the finished product, recurrent use and scale-up in various reactor configurations. The hydrolysis rate data exhibit Michaelis-Menten kinetics. The Michaelis constant were determined using Langmuir linearized plot which obtained vmax and Km as 20.88 μmol/mL.min and 0.0125 mol/L respectively. However, the immobilized β-Glucosidase did not show a sufficiently good operational stability on reuse. Keywords: Immobilized enzyme, enzyme activity, Michaelis-Menten kinetics, β-glucosidase, pnitrophenyl- β-D-glucopyranoside (PNPG), alginate gel 2013-07-02 Proceeding Paper PeerReviewed application/pdf en http://irep.iium.edu.my/48525/1/ICBIOE-2013-PERFORMANCE_OF_%CE%B2-GLUCOSIDASE_IMMOBILIZED_ON_CALCIUM.pdf application/pdf en http://irep.iium.edu.my/48525/4/1175_Acceptance.pdf Jameel, Ahmed Tariq and Yusof, Faridah and Johana, Syaira (2013) Performance of β-glucosidase immobilized on calcium alginate beads. In: The Third International Conference on BIOTECHNOLOGY ENGINEERING ICBioE’13, July 2-4, 2013, Kuala Lumpur, Malaysia. http://www.iium.edu.my/icbioe/2013 |
| spellingShingle | TP Chemical technology TP155 Chemical engineering Jameel, Ahmed Tariq Yusof, Faridah Johana, Syaira Performance of β-glucosidase immobilized on calcium alginate beads |
| title | Performance of β-glucosidase immobilized on calcium
alginate beads |
| title_full | Performance of β-glucosidase immobilized on calcium
alginate beads |
| title_fullStr | Performance of β-glucosidase immobilized on calcium
alginate beads |
| title_full_unstemmed | Performance of β-glucosidase immobilized on calcium
alginate beads |
| title_short | Performance of β-glucosidase immobilized on calcium
alginate beads |
| title_sort | performance of β-glucosidase immobilized on calcium
alginate beads |
| topic | TP Chemical technology TP155 Chemical engineering |
| url | http://irep.iium.edu.my/48525/ http://irep.iium.edu.my/48525/ http://irep.iium.edu.my/48525/1/ICBIOE-2013-PERFORMANCE_OF_%CE%B2-GLUCOSIDASE_IMMOBILIZED_ON_CALCIUM.pdf http://irep.iium.edu.my/48525/4/1175_Acceptance.pdf |